盐酸胍

改良盐酸胍法提取脐血DNA用于HLA基因分型

Modified Guanidine Hydrochloride Method for DNA Extraction from Cord Blood Used in HLA Genotyping

盐酸胍对反胶束中RNaseA活性的影响

Effect of Guanidine Hydrochloride on the Activity of RNase A in DAB Reverse Micelles

猪心乳酸脱氢酶（H4）在盐酸胍变性过程中失活与内源荧光变化速度的比较

Comparison of the rates of inactivation and intrinsic fluorescence changes of lactate dehydrogenase H4 during guanidine denaturation

通过这种方法，研究了温度，pH值，离子强度，尿素，盐酸胍以及肝素对这种类凝血酶的胰蛋白酶活性影响。

Through this method , factors as temperature , pH value , ion concentration , Urea , Guanidine hydrochloride , and Heparin were researched ;

方法：应用乙酸和盐酸胍提取法获取牛牙骨质附着蛋白，鉴定纯度后进行N末端氨基酸序列分析。

Method : Acetic acid and guanidine HCl were selected to extract the bovine CAP . After estimating the purity of CAP , the N-terminal amino acid sequence of CAP was determined .

用计量置换参数Z对疏水色谱流动相中存在盐酸胍时蛋白质分子构象变化进行了表征。

The Z value of the stoichiometric displacement model for retention ( SDM-R ) was used to characterize the changes in the molecular conformations of proteins .

结论抗体沉淀盐酸胍一步法较其他两种RNA病毒核酸提取法具有明显的优势，具有应用价值的新的RNA噬菌体核酸的提取方法；

Conclusion The anti-serum-carbamidine hydrochloride-one-step assay is one kind of new assay who has advantage obviously than other tradition assay to extract the nucleic acid from RNA phage .

盐酸胍变性的蛋白质复性后的质量回收率和活性回收率均高于尿素，SDS变性的蛋白质构象难以恢复，复性效果最差。

The results showed that the refolding efficiency of proteins denatured by GuHCl is the highest , and SDS is the lowest in the three denaturing agents .

温度降低，分子内部驰豫行为降低。在用盐酸胍变性的情况下，随着盐酸胍浓度的增加，旋转相关时间τc逐渐降低，可见蛋白内部的立体结构松散，构象已经松弛。

In the case of using guanidine HC1 denaturalize , with the increase of guanidine concentration , the τ _ c of spin label decreased , this indicate that the inner stereo-structure of protein has loosen , and the configuration has relax .

以鲨鱼软骨为原料，经盐酸胍抽提，丙酮分级沉淀，超滤等步骤得到鲨鱼软骨制剂（sharkcartilagepreparation，SCP）。

Shark cartilage preparation ( SCP ) was extracted from shark cartilage by the method of guanidine hydrochloride extraction , acetone fractional precipitation , and ultrafiltration .

盐酸胍对文昌鱼Branchiostomabelcheri（Gray）酸性磷酸酯酶构象与活力的影响

Relation between comformation and catalytic activity of ACPase from Branchiostoma belcheri ( Gray ) in guanidine hydrochloride denaturation

盐酸胍的加入使Trp残基的荧光发射峰位明显红移，表明位于AaH分子较疏水环境的Trp残基相对外露。

The red - shifted emission band of Aa H after adding guanidine - HCl showed that Trp residues exposed in polar solvents .

本文讨论了经3M盐酸胍变性的兔肌肌酸激酶复性和复活的动力学过程，对二者进行了比较，以期从量的关系上研究酶的构象与催化活力之间的关系。

A comparative study of renaturation and reactivation kinetics of the guanidino denatured creatine kinase has been made in order to corelate changes in conformation and in the catalytic activity of this enzyme .

以丙二酸二乙酯和盐酸胍为起始原料，经环合、亚硝化、还原、氯化、水解得到2-氨基4,6-二氯-5-甲酰氨基嘧啶，总收率20%，其结构经1HNMR和IR表征。

N - ( 2-amino-4,6-dichloro-5-pyrimidinyl ) formamide was synthesized from diethyl malonate and guanidine hydrochloride via cyclization , nitrosation , reduction , chloridization , hydrolysis with an overall yield of 20 % . The structure was characterized by 1H NMR and IR .

本文测定了不同脲、盐酸胍浓度下，TritonX-100溶液的临界胶团浓度（cmc）和肌酸激酶分子在溶液中的暴露巯基数。

The critical micelle concentration ( cmc ) of Triton X-100 and the number of exposed thiol of creatine kinase have been measured with urea and guanidine hydrochloride ( GHCl ) separately as additives .

利用蛋白质内源荧光和酶活性两种信号以及荧光偏振，HPLC和停流等方法研究了盐酸胍诱导的α淀粉酶去折叠与重折叠的平衡转变和动力学。

The unfolding-refolding transition and the time course of a amylase induced by guanidine hydrochloride ( GuHCl ) have been studied with two different signals : the fluorescence and activity of the enzyme as well as by the methods of fluorescence polarization , HPLC and stopped-flow .

采用盐酸胍法从经PHA诱导的小儿扁桃体淋巴细胞中提取总RNA，经Oligo（dT）-纤维素柱层析制备了Poly（A）-RNA。

The total cellular RNA was isolated from the PHA-induced children tonsil lymphocytes by Guaniding · HCl method . The poly ( A ) - RNA was prepared by the oligo ( dt ) - cellulose affinity chromatography .

乌贼神经干经约含0.01M盐酸胍的海水溶液浸泡后，可出现自发发放或由单一刺激引起的重复发放。

Soaking in sea water containing 0.01 M guanidine caused the sepia nerve to give spontaneous discharges and repetitive responses to a single stimulus .

利用盐酸胍浓度梯度凝胶色谱柱复性法从大肠杆菌表达的重组人干扰素α-2b（rhIFNα-2b）包涵体的溶解液中复性rhIFNα-2b。

The recombinant human interferon α - 2b ( rhIFN α - 2b ) expressed as inclusion bodies in E. coli was renatured and purified simultaneously by guanidine hydrochloride ( Gu-HCl ) gradient gel filtration chromatography .

目的观测人胎盘碱性磷酸酶（HPAP）在盐酸胍变性和复性过程中构象与活力的变化，探讨其折叠机制。

Objective To examine the changes of conformation and activity of human placental alkaline phosphatase ( HPAP ) in denaturation and renaturation of guanidine chloride , and to study its folding mechanism .

本文以盐酸胍作为衍生试剂、离子排阻柱AminexHPX-87H为分析柱，采用高效液相色谱柱后衍生法测定兔血清中的911。

Using post-column fluorescent derivation HPLC to analyze 911 in rabbit serum , using guanidine hydrochloride as derivative reagent and ion exclusion column Aminex HPX-87H as analytical column .

以盐酸胍、尿素、碱为试剂对PT-25进行耐温浸泡试验，经蛋白质脱盐试验，该凝胶在试验的前后的蛋白回收率均达到90%以上，达到了国外同类产品的物理稳定性和化学稳定性指标。

The protein desalination experiments combined with the above tests showed that recovery of protein was over 90 % in each experiment . The property of PT-25 was same as Sephadex G-25 , indicating that it can be used in biopolymer seperation with prolonged stability .

盐酸胍中微量砷的光度测定

Spectrophotometric Determination of Micro Amount Arsenic in Guanidine Hydrochloride with DDTC-A

盐酸胍浓度对变性溶菌酶复性的影响

Effect of Guanidinium Chloride Concentration on the Renaturation of Denatured Lysozyme

盐酸胍诱导的淀粉液化芽孢杆菌α-淀粉酶去折叠过程的研究

Unfolding of Bacillus Amyloliquefaciens α - Amylase Induced by Guanidine Hydrochloride

盐酸胍法测定磷酸盐中氧-18的含量

On the guanidine hydrochloride method for determination of oxygen-18 content in orthophosphate

盐酸胍对叶绿体膜能量传递的影响

Effect of guanidine hydrochloride on energy transfer of chloroplast membranes

脲或盐酸胍溶液中变性溶菌酶复性过程中集聚体的研究

Study on Aggregate for Denatured Lysozyme Renaturation in Urea and Guanidine Hydrochloride Solutions

天冬氨酸酶在盐酸胍变性时的酶活性与构象变化

The Conformational Changes of Aspartase in Guanidine Hydrochloride Denaturation

盐酸胍对人胎盘碱性磷酸酶变性与复性的影响

Effect of guanidine hydrochloride on denaturation and renaturation of human placental alkaline phosphatase