视细胞

糖尿病大鼠视网膜视细胞超微结构病理变化的研究

Study on ultrastructural pathological change of visual cell in diabetic rats and its clinical significance

腺病毒介导神经生长因子对遗传性视网膜变性RCS大鼠视细胞的营救

Adenovirus mediated nerve growth factor rescues photoreceptor in RCS rats

RCS大鼠视细胞超微结构观察

Photoreceptor ultrastructure in RCS rat

目的探讨大鼠糖尿病视网膜病变（DR）早期视网膜毛细血管及视细胞（视感受器）的病变规律。

Objective To explore the law of pathologic changes in retinal capillaries and visual cells ( visual receptors ) in rats with diabetes .

用流式细胞术检测视细胞的凋亡率及bcl-2基因蛋白表达量的变化。

Three days after the damage the apoptosis rate of photoreceptor cell and the expression content of bcl-2 were detected by flow cytometry .

方法选择健康成年Wistar大鼠，腹腔内注射链脲佐菌素（STZ）诱导大鼠糖尿病，在透射电镜下观察DR早期视网膜血管及视细胞的超微结构改变。

Methods Healthy adult Wistar rats were chosen for abdominal injection of STZ to induce diabetes and then changes in retinal ultrastructure was observed under TEM .

结果RCS大鼠视网膜视细胞自生后第25天出现凋亡，第30～35天达高峰；

Results The photoreceptor of RCS rats un - derwent apoptosis from 25th postnatal day , and reached the highest level at 30 - 35th postnatal day s.

结论辅酶Q10对光损伤引起的视网膜损害及视细胞凋亡有较好的防护作用。

Conclusion Coenzyme Q10 plays an important role in preventing the photic injury of retina and optic cell apoptosis .

方法用视细胞外节膜盘（ROS）于37℃孵育培养的RPE细胞，在孵育不同终止吞噬反应。

Methods The cultured RPE cells were incubated with rod outer segments ( ROS ) at37 ℃, the phagocytosis was terminated at different incubation time points .

5MmMn~（++）抑制视细胞摄入HRP，为HRP标记的突触泡小水平与明适应时相似。

5 mM Mn ~ ( + + ) inhibits the uptake of HRP by the rod synaptic vesicles to the same extent as that due to light adaptation .

综述了NGF在神经系统和视网膜中的细胞保护作用、机制及应用方面的进展，为其进一步用于视网膜脱离后抗视细胞凋亡治疗提供理论基础。

This paper reviewed advances in the cellular protective action , mechanism and application of NGF in nerve system and retina , which provide a theoretical basis in treatment of the apoptosis of photoreceptors after RD.

目的通过成功纯视细胞移植，观察RCS鼠视皮质内抑制性神经递质γ-氨基丁酸（GABA）的分布和变化。

Objective We observe the γ - aminobutyric acid ( GABA )′ s variation in the visual cortex of Royal College of Surgeons Rat ( RCS rat )′ s retina following the photoreceptor cell transplantation .

AG治疗组大鼠视网膜AR含量明显低于糖尿病未治疗组，糖尿病90天大鼠视网膜视细胞外节膜盘少量排列紊乱，内节线粒体正常。

The quantity of AR in the retina of diabetic rats treated with Aminoguanidine was lower than diabetic rats . In the 90 d diabetic rats treated with Aminoguanidine , the membrane disc of visual cells was partly in disorder and the mitochondria of inner segments was normal .

在这种利伯先天性黑蒙中，视细胞由于基因RPE65不能适当地产生一种对健康视力必需的蛋白而不能感光。

In this form of LCA disease , photoreceptor cells cannot respond to light because a gene called RPE65 does not properly produce a protein necessary for healthy vision .

在37℃、5%C02条件下的恒温培养箱中进行培养，每12-24h换液1次，视细胞生长情况，在细胞接近铺满培养瓶或培养板底时（4-5天后）进行传代。

At 37 ℃, 5 % CO2 conditions in the incubator , each of the 12-24h change 1 time depending on cell growth , in cell close to the shops flasks or training Board ( 4-5 days ) for generations .

视网膜光损伤与视细胞凋亡有着密不可分的联系。

Photic injury and optic cell apoptosis had impartible relationship .

视细胞变化不明显。

As mentioned above , the change of visual cells was not obvious .

结果：缺血再灌注组大鼠视网膜视细胞外节膜盘肿胀，排列紊乱；

Results : The membrane disc of visual cells was swollen and in disorder .

目的观察遗传性视网膜变性大鼠视细胞超微结构改变。

ObjectiveTo observe the ultrastructural alterations of the photoreceptor in inherited retinal degeneration in rats .

大鼠视网膜光损伤与视细胞凋亡关系的研究

A Study on the Relationship of Optic Cell Apoptosis and Experimental Photic Injury in Rats

结果受损的视细胞修复，视网膜形态结构渐趋。

Results The retinal lesions were repaired and the morphological structure of the retina was brought to normal gradually .

电镜下主要病变在视细胞外节及节细胞，视细胞外节基底部新生的膜盘模糊；

Under the transmission electron micro-scope , the main pathological changes involved the external segments of photoreceptors and ganglionic cells .

糖尿病早期大鼠视网膜视细胞超微结构改变与醛糖还原酶的相关性及氨基胍的保护作用

Correlation between ultrastructural changes of visual cells and aldose reductase in retina of early diabetic rats and protection of Aminoguanidine on them

目的探索用准分子激光切削技术制备视网膜单层视细胞植片，经内入路视网膜下腔的单层视细胞成片移植。

Objective To explore the preparation of monolayer photoreceptor sheet via excimer laser ablation and transplantation of monolayer photoreceptor sheet into subretinal space through intraocular approach .

结论在遗传性视网膜变性的视网膜，诱生型一氧化氮合酶激活可能在视细胞凋亡中起诱导作用。

Conclusion In the retinas of inherited retinal degeneration of RCS rats , nitric oxide generated by iNOS might play a significant role in photoreceptor apoptosis .

目的研究地塞米松对光损伤及视细胞凋亡的防治作用，以探讨光损伤视细胞凋亡的发生机制。

Obiective To explore the prevention or cure effect of dexamethasone on the retinal photic injury and optic cell apoptosis , so as to probe into the generation mechanism of optic cell apoptosis .

糖尿病90天大鼠视网膜的视细胞外节膜盘排列紊乱，间隙扩大，内节线粒体水肿，有的空泡化；

In the visual cells of 90 d diabetic rats , the membrane disc was in disorder , membrane disc spaces was enlarged and the mitochondria of inner segments was swollen and even denatured .

结果切削后所得视细胞植片由单层视细胞组成，结构完整，包括外丛状层、外核层和外节层；

Results All paris of the photoreceptor cell in the sheet maintained intact , including the outer segments , inner segments , outer limiting membrane , outer nuclear layer , and outer plexiform layer .

并在第90天时取视网膜行透射电镜观察视细胞超微结构。

The content of AR in the retina of all rats were quantified after 30 and 90 d , the ultrastructure of retinal visual cells were observed by transmission electron microscope 90 d after injection .

视细胞与神经节细胞核比值平均为2.5：1，表明扬子鳄的组织结构与其弱光视觉相适应。

The ratio of the visual cells to the ganglion cells is about 2 . 5:1 . This study shows that the histological structure of retina in Chinese Alligator is related to the visual in dark .