衣壳蛋白
- 名capsid protein
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N端融合外源蛋白的兔出血症病毒衣壳蛋白自聚能力的研究
Study on the self-assemble ability of expressed capsid protein of Rabbit haemorrhagic disease virus that fused with foreign epitope at the N-terminus
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人乳头瘤病毒16型主要衣壳蛋白L1的纯化
Purification of human papillomavirus type 16 major capsid protein L1
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鸡贫血病毒衣壳蛋白基因的PCR扩增和克隆
Amplification by PCR and cloning of the capsid gene of chicken anaemia virus
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通过基因枪和农杆菌共培养法尝试将黄瓜花叶病毒的衣壳蛋白基因转化香蕉组织,其结果表明:经过卡那霉素或G-418的筛选,仅有5%-7%的芽能够存活下来。
Coat Protein ( CP ) of cucumber mosaic virus was transformed into banana tissues by gene gun bombardment and agrobacterium - mediated culture .
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逆时针的一簇主要为噬菌体的DNA代谢、复制、重组、转录相关蛋白,顺时针一簇主要是噬菌体的结构衣壳蛋白。
DNA metabolism , replication , recombination and transcription-related proteins mainly distributed on counterclockwise cluster , while phage structure and capsid proteins were lined on clockwise cluster .
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C型口蹄疫病毒衣壳蛋白前体P1基因的原核表达及其生物活性的初步分析
Expression of the Capsid : Precursor Polypeptide P1 of Foot-and-mouth Disease Virus Type C and Analysis of Its Biology Activity
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脂质体的脂组成、日本凝血病毒衣壳蛋白包裹、与腺病毒的结合物及DNA核靶向序列影响其基因转移的效果。
Liposomes'transfection is affected by lipid composition , coating by capsid of hemagglutinating virus of Japan , lipoadenofection by combination of adenovirus , and DNA nuclear targeting sequence .
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这说明,本研究用纯化的MCP融合蛋白制备兔抗OGNNVMCP抗体是成功的,并证实了OGNNV主衣壳蛋白的免疫原性。
The antibodies was successfully prepared with purified recombinant MCP .
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根癌农杆菌介导甲型肝炎病毒(HAV)衣壳蛋白融合基因转化柑桔的研究
Agrobacterium-mediated Transformation of Citrus with Hepatitis A Virus Capsid Protein Fusion Gene
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芜菁花叶病毒衣壳蛋白亚基分子量测定及其在低浓度SDS中的解聚现象
Molecular weight determination of capsid protein subunit in Turnip mosaic virus and its depolymerization in low concentration of SDS
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赤点石斑鱼诺达病毒的纯化及其衣壳蛋白的Western-blot分析
Purification and Western-blot assay of the capsid protein of the red-spotted grouper nodavirus
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HPV的电泳分析结果表明来自寻常疣、跖疣的HPV具有不同的结构蛋白图型,其主要和次要衣壳蛋白多肽的分子量具有明显的差异。
The electrophoretic analysis results of HPV indicate that there are different polypeptides patterns and molecular weights between verrucae vulgaris and plantaris .
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近年来国外开始把B19的衣壳蛋白作为疫苗抗原进行研究。
Recently recombination of B19 capsid protein as vaccine antigen is processing in fremdness .
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替换HIV-1衣壳蛋白基因SHIV的构建及其活性测定
Construction and Characterization of SHIV Carrying HIV-1 Capsid
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对其中的24株进行PCR检测,结果表明阳性率达83.3%;进一步的Southernblot检测表明:PCR检测阳性的81.8%植株含有外源衣壳蛋白基因。
83.3 % of 24 transformed plants showed positive in PCR analysis , and further Southern blot analysis indicated that 81.8 % of PCR positive plants contained foreign CP gene .
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方法基于噬菌体小衣壳蛋白(gpⅢ)构建12氨基酸随机肽库。
Methods A random peptide library of 12 amino acid residues was established based on a fusion to gp ⅲ of filamentous phage .
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方法采用荧光素标记的羊抗鼠IgG抗体,检测病毒抗原与抗风疹病毒衣壳蛋白E1、E2、C蛋白的单克隆抗体的结合物。
Methods Fluorescent goat anti mouse IgG conjugate was used to detect the complex of Rubella antigens and Rubella monoclonal antibodies to virion protein E_1 , E_2 , and C.
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人乳头瘤病毒HPV-16衣壳蛋白的结构特征
Structural Features of Human Papillomavirus Type 16 Capsid Proteins
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目前研究表明,虽然已成功实现对病毒衣壳蛋白(CP)等基因的转化和Ctv等抗性基因的标记,但尚未获得对CTV有高度抗性的转基因柑橘,而抗性基因亦不能实现定点克隆和转化。
It is indicated that neither transgenic citrus against CTV nor mapping clone and transformation of resistant genes has obtained .
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HIV-1衣壳蛋白抑制剂CAP-1衍生物的合成
Study on Synthesis of Capsid Protein Inhibitor CAP-1 Derivatives
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将纯化的衣壳蛋白免疫新西兰大白兔,制备抗血清,并通过间接ELISA方法对抗血清效价进行测定。结果表明该抗血清效价可达到1:10000,符合酶联免疫检测的要求。
The capsid protein was used to immune New Zealand rabbit with the antiserum prepared . The titer of the antiserum was 1:10000 as was determined with indirect ELISA ( enzyme linked immunosorbent assay ) .
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纳米银溶液作用噬菌体MS2衣壳蛋白的分析
Analysis of effect of silver nanoparticle aqueous solution on the coat protein of bacteriophage MS2
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浙江省尖锐湿疣患者主要感染HPV-6型,病灶中HPV高频率表达病毒主要衣壳蛋白L1。
The pointed condyloma patients in Zhejiang area mainly infected with HPV-6 . The HPV in the focus frequently expressed major capsid protein L1 .
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猪圆环病毒Ⅱ(PCV2)衣壳蛋白羧基端基因在T4噬菌体表面的展示
Display of the Carboxyl Terminus Gene Fragment of Porcine circovirus Type 2 ( PCV2 ) Capsid Protein on the T4 Bacteriophage Surface
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尽管RV的主要三种结构蛋白&包膜糖蛋白E1、E2和衣壳蛋白C都有着较高的免疫原性,在人体内可诱发强烈的抗体应答。
The main three RV structural proteins - the envelope glycoprotein E1 , E2 and capsid protein C have higher immunogenicity in the human body , and can induce a strong antibody response .
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兰州百合病毒病害调查研究及LSV,CMV衣壳蛋白表达纯化
An Investigation in Viral Disease of Lanzhou Lily ( Lilium Davidii Var. Unicolor ) and Expression , Purification of Coat Protein of LSV and CMV
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这些策略包括利用植物自身的抗病毒基因;利用病毒衣壳蛋白基因、运动蛋白基因、复制酶基因、卫星RNA、反义链RNA和缺陷干扰型RNA;
These strategies include host gene mediated resistance strategies and pathogen derived resistance strategies , which involve viral coat protein gene , movement protein gene , replicase gene , satellite RNA , antisense RNA , and defective interfering RNA .
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由于病毒的组成相对较为简单,最适合用软电离质谱进行研究,MALDITOFMS和ESIMS已被成功地用于病毒衣壳蛋白相对分子质量测定、动力学特性、转录后修饰以及突变等方面的研究。
Some researchers have successfully utilized MALDI-TOF-MS and ESI-MS to the studying of viral capsid proteins , including measuring molecule weights , exploring dynamic properties , characterizing post-translational modifications and mutations .
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HPV主要衣壳蛋白L1基因原核表达系统的构建及尖锐湿疣组织标本中HPV-6/11型感染率和L1基因表达率的研究
The Construction of Prokaryotic Expression System of HPV Major Capsid Protein L1 Gene and the Frequencies of HPV-6 / 11 Infection in Biopsy Samples and L1 Gene Expression of Pointed Condyloma Patients
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预测和注释了45个开放读码框(ORF),分析了DNA复制基因、衣壳蛋白和DNA包装基因、侵染相关基因。
Putative forty-five open reading frames ( ORF ) were annotated , homology analysis were used to assign possible functions for the genes , including , DNA replication , synthesis of capsid protein , DNA packaged genes , and infection-associated genes .