葡聚糖酶

方法将前体药物分别与不同pH值缓冲液、含葡聚糖酶的缓冲液及含大肠杆菌的培养基于37℃水浴中一起孵育，采用HPLC法测定前体药物释放泼尼松龙的情况。

METHODS After the prodrug was incubated at 37 ℃ with buffers of different pH , buffers with dextranase and with culture medium containing bacterium coli respectively , the release of prednisolone ( PL ) from prodrug were determined by HPLC .

Mozzarella干酪生产用菌种的筛选β-葡聚糖酶高产菌株的分离筛选及其新菌种初步鉴定

Selection of Mozzarella cheese strains Separation & Selection of High-yield β - dextranase Strains and the Preliminary Identification of the New Microbial Species

亚家族B及亚家族A中的水稻β-葡聚糖酶基因均与防卫反应相关

Both Subfamily A and Subfamily B β - Glucanase in Rice are Inducible by Pathogen Infection

分离得嗜热毛壳菌β-1,3-葡聚糖酶的最适反应温度和pH分别为60℃和6.0。

The optimum pH and temperature obtained for the beta-1,3-glucanase was 6.0 and 60 oC .

pH值对巴西橡胶树胶乳β-1,3-葡聚糖酶结合黄色体膜的影响

Effect of pH Value on the Conjunction Between β - 1,3-Glucanase and Lutoid Membrane in the Latex of Hevea brasiliensis

结果表明已克隆到邦2菌纤维素酶系中的内切葡聚糖酶基因（ED基因）片段。

The results showed that the endoglucanase gene has been cloned .

爪哇根结线虫-β1,4-内切葡聚糖酶基因cDNA全长克隆和序列分析

Cloning and sequence analysis of full-length cDNA of β - 1,4-endoglucanase genes of Meloidogyne javanica

燕麦β-1,3-葡聚糖酶Ⅱ基因3′端cDNA的克隆及分析

Cloning and Sequence Analysis of β - 1,3-glucanase ⅱ Gene 3 ′ End cDNA of Oat

β葡聚糖酶高产菌Bacillussubtilis91266的发酵性能研究

Studies on Fermentation of high β - glucanase producing strain Bacillus subtilis 9126-6

用NorthernBlot，westernblot和酶活测定的方法检测了转基因水稻中几丁质酶和葡聚糖酶的表达和活性。

The expression level and enzymic activity of transgenic rice were evaluated by Northern blot , Western blot and the enzyme reaction .

枯草芽孢杆菌突变株CW－06产生β－葡聚糖酶的纯化及其性质的初步研究

Studies on Purification and properties of β - glucanase produced from Bacillus subtilis mutant cw-06

一般来说，Bt毒素蛋白和葡聚糖酶等蛋白质对蜂类没有影响；

Generally , proteins such as lepidopteran-specific Bt toxins and glucan-degrading enzymes have no effects on bees .

其中温度、初始pH、平板厚度及去氧胆酸钠的浓度都影响曲霉产β－葡聚糖酶分解底物所形成水解圈大小及透明度。

The temperature , initial pH and thickness of the identifying plate affect the size and transparency of the hydrolyzed circle caused by β - glucanase .

烟草Ⅰ类几丁质酶和β-1,3-葡聚糖酶cDNA植物双价表达载体的构建及转化番茄的研究

Construction of Plant Bivalent Expression Vector of Tobacco ⅰ - Chitinase and b-1 , 3-Glucanase cDNA and Its Transformation into Tomato

PAGE活性染色结果显示，P6可分泌两种主要的过氧化物同工酶和多种酯酶，未检测到内葡聚糖酶和木聚糖酶活性。

PAGE activities staining showed that P6 could excrete two major peroxidase isoenzymes and several esterase isoenzymes .

其与防卫反应有关的POD酶、PAL酶、几丁质酶和β-1，3-葡聚糖酶酶活升高。

The enzyme activities associated with defense response were increased , including POD , PAL , chitinase and 6-1,3-glucanase .

在培养条件方面，里氏木霉产木聚糖酶和β-葡聚糖酶的最适起始pH值分别是4.0和5.0，最适发酵温度均为30℃。

The optimal initial pH and fermentation condition was 4 . 0 at 30 ℃ for xylanase production and 5 . 0 at 30 ℃ for β - glucanase production .

枯草芽孢杆菌产生在β－葡聚糖酶的研究：高产突变株CW－06的选育及其发酵条件的优化

Studies on β - glucanase produced by Bacillus subtilis : screening breeding and optimization of fermentation conditions for mutant cw-06

Tween80解除乙醇对内切葡聚糖酶（Cx）的抑制作用

The Protection of Tween 80 for Endoglucanase Against the Inhibition of Ethanol

本试验采用DNS法对某种微生物产β-葡聚糖酶的酶学性质进行研究。

Some general biochemical properties of β - glucanase generated from one sort of microorganism were investigated through the method of DNS .

与诱导抗性有关的指标PAL活性、AsA含量、β-1，3-葡聚糖酶活性和外切几丁质酶活性的变化具有一定的相关性。

And , they were also fairly related to the PAL activity , AsA content , β - 1,3-glucanases activity and exo-chitinases activity .

根据前期获得的小麦β-1,3-葡聚糖酶cDNA全长序列设计特异引物，通过反转录PCR（RT-PCR）获得小麦β-1,3-葡聚糖酶基因的编码区。

The encoding region was engineered via reverse transcription polymerase chain reaction ( RT-PCR ), using specific primers designed based on a wheat β - 1,3-glucanase gene full length cDNA acquired previously .

结果表明PAL，PPO，几丁质酶和β-1,3-葡聚糖酶的活性变化与小麦对白粉的抗性密切相关。

The research indicated that the activity of PAL , PPO , chitinase and β - 1,3-glucanase were in significant relation with the induced resistance .

从中克隆了8株芽胞杆菌属菌株的内切葡聚糖酶（EG）基因，进化树分析表明它们在核苷酸和蛋白质水平上分属不同的分支，具有一定的多样性。

Eight endo - β - 1 , 4-glucanase ( EG ) genes were cloned from Bacillus strains and showed different homology by DNA or protein phylogenetic tree analysis .

分别以多孔陶瓷和浮石为载体吸附法固定重组大肠杆菌（EscherichiacoliJM109-pLF3）表达β-葡聚糖酶。

In order to improve the productivity of β - glucanase , porous ceramic carrier and broken pumice were applied to immobilize recombinant Escherichia coli JM 109 ( pLF3 ), respectively .

在非适宜季节饲料中涂加500g／T、β-葡聚糖酶可以减轻应激，提高饲料能量利用率，降低饲料成本，提高经济效益。

Using B - dextran enzyme in the feed can reduce the stress in unsuitable season .

增强了幼苗的过氧化物酶（POD）活性，诱导了病程相关蛋白（即，几丁质酶和β-1,3-葡聚糖酶及苯丙氨酸解氨酶）的表达。

, and peroxidase ( POD ) activity of the seedlings , also , induced the expression of pathogenesis-related protein ( ie , chitinase and β - 1 , 3-glucanase and phenylalanine ammonia lyase ) .

同时，复合处理较单一处理更为有效地诱导了草莓果实中β-1,3-葡聚糖酶、几丁质酶和PAL活性上升，从而提高了果实的抗病性。

Meanwhile , the combined treatment induced higher activities of the defense-related enzymes including β - 1,3-glucanase , chitinase and PAL in strawberry fruit than applying either alone to enhance the resistance .

本课题以β-葡聚糖酶水解茯苓多糖制备水溶性茯苓多糖（watersolublepachyman，WSP），并初步研究了WSP的物理化学性质和抗肿瘤活性。

In this subject , pachyman is hydrolyzed with β - glucanase to get water soluble pachyman ( WSP ), and the physical and chemical properties and antitumor activities of WSP are studied .

水稻编码病程相关蛋白PR-4和葡聚糖酶基因的克隆与鉴定

Cloning and Characterization of Genes for Patho genes is - Related Proteins in Rice