花粉培养

对于花药培养和花粉培养，采用低温预处理花序（4℃下1～2d）和高温预培养（33℃下1～2d），均能促进胚状体的形成，提高产胚率。

In anther and pollen culture , using the low temperature treating anthotaxy ( 4 , 1 ~ 2day ) before being inoculated and high temperature culturing ( 33 1 ~ 2day ) after being inoculated can promote the embryogenesis and increase embryoid rate .

茄果类蔬菜花药和花粉培养研究进展

Progress of Anther and Pollen Culture of Solanum Vegetable Crops

花粉培养过程中花粉壁渗出的Ca（2+）量与萌发率和花粉管长度呈极显著正相关。

The Ca2 + amount released from pollen wall was positively correlated significantly with pollen germination and tube length during pollen incubation .

花粉培养的发芽率较低；

The rate of the germination pollen cultured is very low .

水稻游离花粉培养诱导形成绿色植株及有关作用因素的研究

Green Plants Regenerated from Isolated Rice Pollen Grains in vitro and the Induction Factors

但因花药或花粉培养过程较为复杂，且培养过程中易产生无性变异，因而制约了其大规模应用。

However , the complex process and higher frequency of colonel variation make anther or pollen culture quite difficult in large scales application .

小麦花粉培养中蔗糖浓度外源激素对启动细胞分裂的作用

An Analytic Study of the Sucrose Concentrations , the Exogenous Hormone and the Low Temperature Pretreatment Influencing the Initiation of Cell Division in Wheat Pollen Culture

综述了茄果类蔬菜花药和花粉培养的历史、影响因素和培养技术，指出了存在的问题和今后的研究方向。

History effect factors and culture technology of anther and pollen culture of solanum vegetable crops were reviewed . The questions and research orientation in the future were indicated .

主要综述了国内外花药和花粉培养的研究进展，介绍了影响离体雄核发育的因素以及花药培养育种技术存在的问题和发展前景。

This article reviewed the advances of anther culture , including embryogenesis in anther culture , factors effecting anther culture and so on . The paper also put forward some existing problems and research prospects in anther culture .

本文从胚培育种、花药和花粉培养育种、原生质体培养与细胞融合、基因工程育种四个方面综述了生物技术在果树育种上的运用，并对其未来的发展作了展望。

This article summarized the application of biotechnology breeding in fruit trees from embryo culture , anther and pollen culture , protoplast culture and cell fusion , gene engineering breeding . The direction which needs further investigation was also discussed .

采用花粉液体培养法研究不同培养基组分和pH值对梨花粉萌发和花粉管生长影响。

The effects of medium components and pH on pollen germination and tube growth in pear by cultivating pollens in liquid medium were studieds .

小麦花粉液体培养成苗的试验

Experiment on shoot formation from wheat pollen by liquid culture

水稻花药和花粉液体培养的研究

The study on Rice Anther and pollen culture in liquid

花粉用培养基培养，观察其萌生花粉管的情况，从而评价花粉生活力是可行之举。

It is a useful method to evaluate viability of sugarcane pollen by culture with artificial medium .

结果表明：花粉离体培养后萌发率随EMS浓度的增加而降低，并与处理当代的结实率呈正相关；

The results were as follows : Pollen germinating rate decreased with the increasing of EMS treatment concentration , and showed positive correlation with seed setting percentage ;

当花粉粒在培养基上萌发120min后，野生型花粉的萌发率几乎为100%，而（-）12株系的仅为20%。

After 120 min , the proportion of germinated pollen grains in WT was nearly 100 % , compared to only 20 % in ( - ) 12 line .

大豆花粉离体培养获得愈伤组织

Callus formation from pollen culture invitro of soybean

外源性糖对四种木本观赏植物花粉离体培养的影响

Effects of sugar on the pollen culture in four woody ornamental plants in vitro

关于水稻花粉的悬浮培养

On the Pollen Suspension Culture in Rice

体细胞无性系变异是继花粉和花药培养之后的又一种实用化的细胞工程育种新方法。

Somaclonal variation breeding was a new applicable method of cell engineering under breeding after pollen and anther culture .

探讨不同培养基上三七花粉愈伤组织培养过程中总皂甙含量的变化情况。

The study aimed to discuss the content changing of total saponin during the callus culture of Panax notoginseng pollens on different media .

花粉在人工培养条件下，其萌发率低，柱头未分泌粘液前和花开放后期花粉萌发率为0.0%，成熟期的花粉萌发率可达5.2%，花刚开放时花粉萌发率最高达10.1%。

The proportion of pollen germination was low under artificial culture . It was 0.0 % before stigma excreted mucilage or at the later stage of blossom . It would be 5.2 % during pollen maturation period , and it could reach about 10.1 % at the beginning of blossom .

东方百合系列花粉离体萌发培养基最佳组分构成为：蔗糖为13%、硼酸143mg／kg、琼脂1%。

In the pollen segregation germination culture medium are sucrose 13 % , boric acid 143mg / kg , agar 1 % .

小麦、水稻、烟草花粉粒离体培养

The culture of pollen grain of wheat , rice and tobacco in vitro

初步研究了番茄未成熟花粉离体成熟培养的可能性。

The possibility of in vitro maturation of tomato immature pollen was studied .

基于响应面法的白蜡属花粉离体萌发培养基优化

Response Surface Methodology to Determine the Optimal Medium for Pollen Germination for Ash

番茄未成熟花粉离体成熟培养及花粉蛋白质检测的初步研究

Preliminary studies on maturation culture of immature pollen in vitro and proteins detection of tomato ( Lycopersicon esculentum ) pollen

油菜花粉萌发率与培养基中蔗糖和硼酸浓度均呈一元二次函数关系。

The relationship between rape pollen germinating rate and concentration of sucrose or boron acid in incubation media was a quadratic function with one unknow quantify , respectively .

具有生命活力的油菜花粉经蒸馏水培养（25℃，20分钟）产生的代谢物质在酸的作用下可迅速外渗，检测具外渗物的花粉数可估计花粉生活力。

Every viable rapeseed when it was incubated in distilled water for 20 min. at 25 ℃, then acted by acid solution , the metabolic products will be flowed out rapidly . By examing and counting the number of pollen carried with exosmosis matter , its viability could be known .

用不同的培养方式时棕榈花粉生活力存在差异，花粉培养密度与花粉生活力存在一定相关性；

The pollen viability by using different culture method had differences and there were a certain correlation between the pollen density and pollen viability .