胞内蛋白

信号从细胞表面传到细胞核是经由“第二信息子”完成的，这种信息子可以是胞内蛋白或小分子如环状AMP、肌醇磷酸盐或钙离子。

The relaying of the signal from the cell surface to the nucleus is mediated by second messengers , which can be intracellular proteins or small molecules such as cyclic AMP , inositol phosphate , or calcium ions .

例子包括涉及指导基因成长和分裂的成长因子和胞内蛋白。

Examples involve the growth factors and the intracellular proteins involved in signaling the genes for cell growth and division .

腺苷受体与G蛋白偶联，通过改变第二信使系统活性来调节胞内蛋白激酶信号通路和调控下游靶基因表达。

Adenosine receptors are coupled to G proteins , though altering the activity of a second messenger system , can modulate protein kinase signaling pathways and regulate downstream target gene expression .

整合素连接激酶（integrin-linkedkinase，ILK）是近年来新发现的一种胞内蛋白激酶，它是整合素通路的关键激酶。

Integrin-linked kinase ( ILK ) is a protein kinase discovered recently , which is the key kinase in integrin pathway .

酵母自溶由酵母胞内蛋白分解酶外泄引起，影响酵母自溶的因素有：（1）酵母菌种；

The release of intracellular album clastic enzyme resulted in yeast autolysis and the causations of yeast autolysis covered : 1.yeast species ;

揭示了毒死蜱能影响细胞的发育及凋亡、胞内蛋白运输、转录调控、信号转导等，使我们对毒死蜱的毒理学有了进一步的了解。

We also revealed chlorpyrifos can affect cell development and apoptosis , intracellular protein transport , transcriptional regulation , signal transduction , ect . Make us have a better understanding to the toxicology of chlorpyrifos .

它参与了多种胞内合成蛋白的运输和分泌，比如说黑色素体依赖于肌动蛋白（actin）的运输等。

It is involved in transports and secretion of many proteins , for example the actin-based movement of melanosomes .

然后通过超声波细胞破碎获得胞内可溶性蛋白，利用镍柱亲和层析法纯化得到电泳纯的重组NTD。

The recombinant NTD was purified from intracellular proteins through sonication and Ni-affinity chromatography .

但CL-L1为唯一发现的胞浆内蛋白，对它在人体组织表达谱及功能了解较少。

CL-L1 is the only cytoplasmic protein , we understand very little about its structure , expression spectrum in body tissues and function .

寻找内毒素和培养的内皮细胞直接结合的证据及对胞内连接蛋白α－Catenin的影响，观察内毒素对内皮细胞的损伤特性。

To explore the evidence of the direct combination of endothelial with cultured endothelial cells and the ef - fects of endotoxin on intracellular α - Catenin ,α - Catenin so as to study the features of endotoxic damage to endothelial cells .

同时伴有蛋白酪氨酸磷酸酶的活性变化，反映了胞内的蛋白酪氨酸残基磷酸化与去磷酸化的即时调节机制。

The change of tyrosine phosphatase activity following the kinase reflects the real time regulation of phosphorylation and de phosphorylation .

银杏叶提取物可提高内皮细胞在高糖环境下的活力，降低其凋亡率，可能与其通过抗氧化作用及下调泛素介导的胞浆内蛋白水解系统有关。

Extract of Ginkgo biloba attenuates the apoptosis of endothelial cells exposed to high glucose concentration , which is at least partly via inhibiting ROS formation and Ub mRNA expression .

目的：探讨钙粘素及其胞内结合蛋白在膀胱移行细胞癌中的表达及其在膀胱癌浸润转移和预后中的价值。

Objective : To study the expression of E - cadherin and its cytoplasmic conjugated proteins in bladder transitional cell carcinoma , and its significance in bladder cancer infiltration , metastasis and prognosis .

我们推断，生长过程中胞内钼铁蛋白聚合态的变化可能是调节固氮酶活力的一种方式。

We suggest that , the octamer of molybdenum-iron protein is a possible metablite in regulation of nitrogenase activity , the conversion from the tetramer to octamer will cause the decrease of nitrogenase specific activity in vivo .

在细胞生长过程中，胞内钼铁蛋白两种聚合体的相对含量出现规律性变化：在对数期，细胞固氮酶比活力成上升趋势，而钼铁蛋白主要以高活力的四聚体形式存在；

The octamer formation in vivo is related to cell growth process . During the log-phase of cell growth , the specific activity of nitrogenase is increasing , and the tetramer is the major form of molybdenum-iron protein .

胞内钙与蛋白激酶C对人脐静脉内皮细胞组织因子途径抑制物及组织因子表达的影响

Roles of intercellar ca ~ ( 2 + ) and protein kinase C played in tissue factor pathway inhibitor and tissue factor expression in human umbilic vein endothelial cells

G-CSF受体胞内区结合蛋白COXⅡ的分子克隆及相互作用验证

Molecular Cloning and Identification of Protein-protein Interaction of a Novel Binding Protein COX ⅱ in the G-CSF Receptor

方法：运用链亲和素-生物素放大系统结合的酶联免疫法，动态检测苦参碱作用于K562细胞后，K562细胞膜相及胞浆内的蛋白酪氨酸激酶和磷酸酶活性的变化。

Methods : ELISA coupled with streptavidin biotin system was used to dynamically detect the activity of protein tyrosine kinase and phosphatase in the cytoplasm and the membrane of K562 cells treated by matrine .

预先给以转录抑制剂更生霉素和翻译抑制剂cycloheximide则能使上调的胞质内AR蛋白分别降至单纯睾酮刺激组水平的46%和12%；

Pretreatment with transcription inhibitor actinomycin D and translation inhibitor cycloheximide repressed cytoplasmic AR protein leves to 46 % and 12 % ( means ) of the androgen treatment control level respectively .

胞内磷酸化蛋白酪氨酸激酶的流式细胞术检测

Detection of intracellular phosphorylated protein tyrosine kinases by flow cytometry

目的探索应用酵母双杂交系统筛选造血因子受体胞内区结合蛋白的可行性。

Objective To investigate the feasibility of searching for proteins which interact with intracellular domain of hematopoietic growth factor receptors using yeast two-hybrid system .

一种改进的同步辐射X射线荧光原位分析人肝细胞胞质溶胶内金属蛋白分布的方法

An Improved Method for Analysis of Distribution of Metalloproteins in Human Liver Cytosol by Synchrotron Radiation X-ray Fluorescence

细胞间连接损伤；E-cadher-in弥漫分布于胞浆内，其蛋白含量持续下降（P<0.05），与B，C组比较，均有显著性差异。

Damages of adhesion junctions E-cadherin was localized at the cytoplasm , the level of E-cadherin protein was markedly decreased ( P < 0.01 ) .

另外，胞内的一些骨架蛋白和信号蛋白也可与非NMDA受体结合而影响其功能。

In addition , some scaffolding proteins and signaling proteins are also able to bind to non-NMDA receptors to influence their functions .

iNOS及eNOS蛋白均在胎儿胎盘绒毛滋养层细胞胞浆内表达，蛋白表达量有显著差异（P0.05）。

INOS and eNOS proteinum were all express in trophoblastic cell of foetal placental villus , the expressed quantity of proteinum is significant deviation ( P0.05 ) .

ColiBL21（DE3）中胞内诱导表达P9ZFD蛋白，组氨酸亲和色谱法纯化P9ZFD蛋白并免疫Balb/c小鼠制备其多克隆抗体。

Coli BL21 ( DE3 ) and purified by histidine affinity chromatography . P9-ZFD antibody was prepared through immunizing Balb / c rats with purified P9-ZFD protein .

将RET胞内域与LexA蛋白融合构建成pGilda-RET~（IC），确认无自激活作用后，将其作为诱饵蛋白，经酵母双杂交方法筛选人脑LexAcDNA文库，获得274个克隆。

The intracellular part of RET receptor was fused to LexA and used as a bait to screen a human brain LexA two-hybrid cDNA library , and 274 positive clones were obtained .

酵母双杂交技术筛选胞内氯离子通道蛋白1结合蛋白

Screening of chloride intracellular channel 1 binding proteins by yeast two-hybrid system

胞内胞外总蛋白的生测结果初步断定10个供试菌株所表现出的杀虫活性物质主要是蛋白类。

The bioassay results showed that the main insecticidal substances of these 10 testing strains were proteins .

人晚期糖基化终产物受体胞质内段融合蛋白表达载体的构建与表达

Vector construction for the intracellular domain of human receptor for advanced glycation endproducts and expression of the fusion protein