肥达反应

伤寒杆菌H抗原检测、肥达反应与细菌培养对伤寒诊断的对照观察

Contrast observation on typhoid bacilli H antigen detection , widal reaction and bacterial culture for diagnosing typhoid

虽然非伤寒患儿肥达反应可呈假阳性反应，但O、H抗体凝集效价极少达到1∶800。

Although non typhoidal febrile children manifested false positive Widal test , agglutinin level at titre of 1 ∶ 800 was only found in a few patients .

方法该文采用细菌培养、肥达反应、伤寒杆菌H抗原酶联免疫检测检查52例伤寒患者，20例正常人，进行对照观察。

Methods : We examined and observed 52 patients with typhoid and 20 normal controls by bacterial culture , Widal reaction , and enzyme-linked immunoassay for detection of typhoid bacilli H antigen .

结果细菌培养、肥达反应、伤寒杆菌H抗原的酶免疫检测对伤寒患者的诊断的阳性符合率分别为34.6%，59.6%和86%。

Results : The positive coincident rate of bacterial culture , Widal reaction , and enzyme-lin ked immunoassay for detection of typhoid bacilli H antigen for diagnosing typhoid were 34.6 % , 59.6 % , 86 % , respectively .

结果：伤寒组肥达反应的肥达反应阳性率为97%（84/87），非伤寒组为4%（40/922），两组比较差异有极显著意义，P0.001。

Results : There was significant difference in positive rate of Widal test between the typhoid fever group and the non typhoidal fever group ( 97 % vs 4 % , P0.001 ) .

LPS-PHA、肥达反应和细菌培养诊断伤寒的比较研究&附102例检测结果

Comparative study on the diagnosis of typhoid with LPS PHA , Widal test and bacteriological culture with results of 102 cases

方法采用微柱凝胶技术（MGCT）及肥达反应法（WR）分别检测32例疑似伤寒及副伤寒病患者。

METHODS Minigel column technique ( MGCT ) and Widal reaction ( WR ) were used to detect the antibody among 32 suspicious typhoid and paratyphoid patients .

肥达反应阳性和假阳性的临床意义

Clinical value of positive and false positive Widal reaction

肥达反应对儿童伤寒诊断价值的再评价&附87例分析

Reevaluation of the diagnostic value of Widal test in children with typhoid fever

方法采用现场流行病学及病例对照方法进行调查，对采集的标本进行肠道致病菌培养和肥达反应检测。

The collected samples were cultured for the identification of intestinal pathogens with Widal reaction done .

肥达反应的阳性率为42.7%（50/117例），其中有27例细菌培养阴性的患儿肥达反应为阳性。

The positive rate of Widal test was 42.7 % ( 50 / 117 ), and it was positive in 27 cases with negative bacterial cultures . 6 .

目的：探讨抗伤寒抗体（St-Ab）、肥达反应和细菌培养诊断伤寒的临床价值。

Objective To explore the clinical value of anti serum typhoid antibody ( St Ab ), Widal ′ s reaction ( WR ) and bacteriological culture in the diagnosis of typhoid .

结论：肥达反应仍是当今诊断儿童伤寒尤其是细菌培养阴性的伤寒患儿的可靠、有意义的辅助性诊断指标，其诊断价值应得到肯定。

Conclusion : Widal test is still considered to be a reliable and significant test for the diagnosis of typhoid fever in children with negative bacterial culture , and its diagnostic value should be confirmed .

血培养仍是伤寒诊断的金标准，肥达反应阳性率低，但对于细菌培养阴性者仍有重要意义。

Blood culture remains the gold standard diagnostic method for diagnosis of typhoid fever . The positive rate of Widal test is low , but it is still of great significance for the cases with negative bacterial culture . 5 .

结果：共检测确诊早期伤寒病人血清21例，肥达氏反应Hd阳性8例，免疫印迹酶联试验Hd阳性21例。

Result The sera from 21 patients with typhoid fever were detected , 8 samples showed posi-tive by Widal 's reaction , but 21 were positive by immunoblot assay .

Dot-ELISA法比肥达氏反应敏感10倍以上，且无交叉反应。

Dot-ELISA was ten times more sensitive than Widal 's reaction , moreover , it had no cross-reaction .

方法选择224例门诊发热疑似伤寒患者，于发病第3、4、7d，分别用ELISA法、LPS-PHA、血培养、肥达氏反应检测伤寒杆菌。

Methods 224 suspicious outpatients with typhoid were tested by ELISA , LPS-PHA , blood culture , Widal test on 3rd day , 4th day , 7th day .

方法采用Dot-ELISA测定伤寒沙门菌包膜抗原V、菌体抗原O和鞭毛抗原Hd的特异性抗体，并与肥达氏反应相比较。

Methods A Dot-ELISA method was used to determine the specific antibodies of envelope antigen , O antigen and H d antigen in Salmonella . Then the results were compared with the results of Widal 's reaction .

结果伤寒杆菌抗原酶免法（ELISA）、LPS-PHA在第7d的检出率分别为85.4%和80.5%，显著高于血培养和肥达氏反应（P<0.05）。

Results The positive rate of immunoassay of typhoid ( ELISA ) and LPS-PHA in typhoid individuals were 85.4 % , 80.5 % on 7th day , both of them were much higher than those of blood culture and Widal test ( P < 0.05 ) .

山西医学院86级学生血清肥达氏反应抗体水平调查

Investigation of Widal test antibodies in students of Shanxi Medical College

薛夫讷氏试验没有确定结果，而肥达氏反应支持为伤寒。患者于12月13日住进鲁奇尔医院。

The Schuffner test was inconclusive but the Widal reaction supported typhoid fever and he was admitted to Ruchill Hospital on13 December .

〔方法〕采用血、骨髓、粪便病原菌培养方法、生化反应、血清凝集实验、肥达氏反应、抗原酶联免疫实验方法和药物敏感性试验。

〔 Methods 〕 The pathogenic bacteria were cultured using the blood and bone marrow excrement biochemistry , serum agglutinin test , Widal reaction , ELISA and medicine sensitivity test .

79例病人仅有49例（62.0%）肥达氏反应阳性，其中伤寒病人的阳性率明显高于副伤寒病人，分别为73.3%与26.3%（P<0.05）；

Only 49 cases ( 62.0 % ) had positive widal test . The positive rate was higher in the patients with typhoid fever than in the patients with paratyphoid fever ( 73.3 % and 26.3 % , respectively ; P < 0.05 ) .