细胞融合

这表明，PEG诱导的鱼类细胞融合具有物种和组织特异性。

This shows that the PEG - induced fish cell fusion has its specificity in relation to species and tissue .

应用细胞融合技术建立抗人IgG4McAb杂交瘤细胞，生产McAb。

Hybridomas producing monoclonal antibodies ( McAb ) against human IgG4 were prepared by cell fusion technique .

B16与活化B细胞融合瘤细胞主动免疫诱导抗肿瘤作用的初步研究

Preliminary study on in vivo antitumor effect of b16 . b

为今后进一步研究G基因的致病性，找到与细胞融合有关的氮基酸区域奠定了坚实的基础。

And it may be helpful in studying the pathogenicity of G gene and finding out the amino acid domain about the cells fusion .

结论转染IL-12后的DC与新建卵巢上皮性癌细胞融合后形成的融合细胞能激活T淋巴细胞，且其体外免疫杀伤效应更强。

Conclusion The cytolytic T cell activity induced by the fusions of ovarian carcinoma cells to transfected DC is enhanced .

风疹病毒E2包膜糖蛋白亮氨酸突变对细胞融合的影响

Effects of mutations at the E2 protein leucine sites on specific membrane fusion in rubella virus

结论：RBC与肿瘤细胞融合制备的细胞可作为瘤苗。

Conclusion : The fused cells of RBC and tumor cell could be used as tumor vaccine .

当突变为R和E时，细胞融合活性未发生改变，但细胞表面表达效率有所下降，分别为野毒株的63%和44%。

While mutated to R or E , there was no change in fusion activity , but expression efficiency decreased to 63 % and 44 % , respectively .

风疹病毒包膜糖蛋白E1中二硫键对其细胞融合活性的影响

Effects of Disulfide Bridges in Glycoprotein E1 on the Membrane Fusion Activity of Rubella Virus

在细胞融合过程中化学聚集电融合法，即用PEG作为聚合剂与电融合法连用，取得明显的效果。

In the course of protoplast fusion , chemocollect-electrofusion was tried in which elec-tronicfusion was used by adding PEG as polymeric agent .

培养14d时，梭形细胞融合成内皮细胞单层。

After 14 days ' culture , endothelial cells monolayer was formed .

细胞融合前反复试验，确定了间接ELISA筛选阳性杂交瘤细胞株的最佳反应条件，阳性血清和阴性血清分别作为阳性和阴性对照。

By repeated experiments before cell fusion to determine the conditions that indirect ELISA positive hybridoma cells selected optimum , the positive serum and negative serum were used as contrast .

目的为了探讨肾综合征出血热（HFRS）患者早期尿细胞融合程度与临床病情预后的关系。

Objective To study the relationship of fusion cells in the urine to renal lesion or prognosis of hemorrhagic fever with renal syndrome ( HFRS ) .

结论通过细胞融合方法可以有效改善肿瘤细胞的B7分子表达，从而提高肿瘤细胞的抗原性。

Conclusion Expression of B7 molecule in tumor cell can be ameliorated through cell fusion , thereby antigenicity of tumor cell will be raised .

Balb/c小鼠背部皮下注射纯化的重组SEI进行免疫，在细胞融合前三天进行冲击免疫。

Balb / c mice were injected subcutaneously on the back with purified recombinant SEI and the animals were boosted with recombinant SEI three days before cell fusion .

应用细胞融合技术和扫描电镜研究了BK（牛肾）细胞早熟凝集染色体（PCC）和诱导PCC的CHO中期染色体的超微结构。

The ultrastructure of prematurely condensed chromosomes ( PCC ) in BK cells and the CHO metaphase chromosomes ( the PCC inducer ) were studied with cell fusion technique and SEM .

本研究摸索出了一种高效YAC内基因打靶的新方法，并探索了通过细胞融合的方法将YAC外源基因导入哺乳动物体细胞内的可行性，这为利用YAC进行转基因动物的生产提供了借鉴。

Explored the method of the YAC transfected into goat fibroblast cell by spheroplast fusion and proved the possibility . of this method .

XR-1型电致细胞融合仪原理

Principle of XR-1 electro cell fusion apparatus

用重组牛bFGF免疫Balb／c小鼠，通过细胞融合，以反向间接血凝和间接ELISA筛选，以及有限稀释法克隆化，建立了9株稳定分泌抗bFGF单克隆抗体（mAb）杂交瘤细胞。

Nine hybridoma cell lines secreting anti bFGF mAb have been developed from immunized Balb / c mice with recombinant bovine bFGF , by cell fusion , screened with reverse indirect hemo agglutination and indirect ELISA and cloned with limited dilution method .

经过两次细胞融合、单克隆抗体的筛选、克隆化、共得到可以稳定分泌单克隆抗体的杂交瘤细胞系6株。其中1株阳性克隆命名为：B8，经亚类检测为IgG1型。

Through 2 times of cell fusion , screen of monoclonal antibodies , cloning , we obtained 6 stable monoclonal antibodies , one of which was named B8 .

分别对3例FA患儿的外周血淋巴细胞进行了丝裂霉素C（MMC）诱导的染色体断裂试验，用细胞融合和互补分析方法，检测FA患儿基因亚型。

The peripheral blood lymphocytes were collected and mitomycin C ( MMC , DNA cross link agent , the concentration 100 ng / ml ) induced chromosomal breakage tests were done . The genetic subtype of FA was achieved by cell fusion and complementation analysis .

目的：肿瘤转移过程有多种因素参与，上皮间质转化（EMT）可能是促进其转移的因素之一，细胞融合在EMT中的作用值得重视。

Objective : Multiple factors involved in the process of tumor metastasis , epithelial to mesenchymal transition ( EMT ) is one of the factors . The role of cell fusion in EMT is worthy of attention .

利用PEG介导的细胞融合方法，将已成系的牙鲆鳃细胞与中国对虾淋巴细胞进行细胞杂交，对所获得的杂交细胞用MEM∶MPS（1∶1）混合培养液进行体外培养。

The hybrid cells produced by PEG mediated cell fusion method with gill cells from flounder , Paralichthys olivaceus , and lymphocytes from shrimp , Penaeus chinensis , were cultured in mixed medium of MEM ∶ MPS ( 1 ∶ 1 ) .

在小鼠和人的体内，它们被Blau和其他学者证明是脑内唯一和骨髓源细胞融合的细胞。

They are the only cell in the brain shown by Blau and others to fuse with these bone marrow-derived cells in mice and humans .

在制备抗猪SIgAMabs中，进行了两次细胞融合，两次的融合率分别为79.7%和60.3%；初检阳性率分别为4.1%和16.8%。

To prepare Mabs against porcine SIgA , cell fusion was made twice . The cell fusion rates were 79.7 % and 60.3 % , and the positive rates were 4.1 % and 16.8 % , respectively .

5d左右细胞融合范围可达70%，9d左右细胞融合达90%，11d左右细胞完全融合形成细胞膜片。角质形成细胞可在体外稳定培养2～3个月，细胞形态和生长速度无明显改变。

Cell fusion amount to 70 % at around 5 days , reaching to 90 % at day 9 and even completely fused into cell diaphragm at day 11 . Keratinocyte cultured in vitro can survive 2-3 months without obvious changes in cell morphology and growth speed .

细胞融合仪是进行电场诱导融合的核心装置，对其研制和改进能促进这项新技术的不断完善。

Electric field-generating unit is the core device for this technique .

目前来说，细胞融合已经是一项比较成熟的技术了。

Now the cell fusion technology is a very mature technology .

细胞融合大鼠肝癌疫苗体内治疗和预防作用研究

Protection and Curative Effect of Cell Fusion Rat Hepatic Tumor Vaccine

植物遗传工程四、细胞融合与遗传转化方法

Plant Generic Engineering IV. Cell Fusion and Genetic Transfer Methods