细胞复苏

我们的研究表明，从光损伤，为AMD公司发生什么干的很好的模型视觉细胞复苏的明确证据。

Our research shows clear evidence of recovery of vision cells from light damage , a good model for what happens in dry AMD .

结果：①5%、15%、20%二甲基亚砜组冻存K562细胞复苏率分别与10%二甲基亚砜组比较，差异显著（86.70%，82.03%，63.09%，88.13%，P均<0.01）。

RESULTS : ① The recovery rate of K562 cells in 10 % DMSO freezing solutions had significant differences compared with 5 % , 15 % and 20 % DMSO freezing solutions ( 86.70 % , 82.03 % , 63.09 % , 88.13 % , P < 0.01 ) .

【结果】玻璃化冷冻组与慢速冷冻组解冻后的细胞复苏率分别为81.9%和22.8%（P0.001）。

[ Results ] 81.9 % of the human ES cell clumps were recovered after vitrification , while only 22.8 % of cell clumps were recovered after slow freezing . The difference was statistically significant ( P 0.001 ) .

细胞复苏后活率较冻存前有所下降，这表明细胞在冷冻、复苏过程中可能受到一定的损伤。

This showed the cell was injured during cryopreservation and revival .

低温处理成纤维细胞复苏过程延迟再水化的初步研究

The preliminary study of delayed rehydration of normal human fibroblast after freezing and thawing

方法分为细胞复苏、细胞培养、细胞传代、细胞冻存四个步骤。

Methods : Divide into cell recovery , cell culture , cell passage and cell cryopreservation .

经液氮冻存的细胞复苏后活性达90%以上。

The viability of the cells recovered from liquid nitrogen was up to 90 % or above .

冻存时间不同的成年动物肝细胞复苏后对重型肝炎胆红素代谢的效果比较

Comparision of effects of adult animal hepatocytes after Resuscitation with different cryopreservation time on bilirubin metabolism of severe hepatitis

冻存细胞复苏后生长曲线良好。

The cell growth curve of frozen cells measured by MTT colorimetry was similar to that of non-frozen cells .

冻存细胞复苏后及体外传代3个月，检测抗体分泌稳定性和克隆阳性率；

The seed cells stored frozen were determined for antibody-secreting stability and cloning positive rate after resuscitation and 3 months after subculture in vitro separately .

实验时均采用同一批冻存细胞复苏后的第三代细胞，以保证细胞性状的稳定性。

We adopted the third generation of cell after revived the same batch of frozen cells , in order to ensure the stability of cell characteristics .

细胞复苏后进行了形态学、细胞活率、细胞生长动力学、同工酶酶谱分析以及荧光蛋白基因表达等细胞质量检测与生物学特性研究。

Cell quality detection and biological characteristics of study were completed after cell recovery , such as , cell morphology , cell survival rate , cell growth kinetics , isoenzyme fluorescent protein gene expression .

结果：生精小管及完整睾丸冷冻复苏后细胞复苏率分别为873%及850%，两复苏率之间无显著差异，与生精小管单细胞对照组相比也无显著差异。

The recoveries of seminiferous tubules and whole testes of 7-day-old mice were 87.3 % and 85.0 % , respectively . There was no significant difference between the two recoveries , and also showed no significant differences compared with single cell control .

结果玻璃化冷冻组MII卵和GV卵母细胞的复苏率及囊胚率均显著高于慢速冷冻组（P0.05）。

Results : The rates of survival and blastulation of MII and GV oocytes in vitrification group were significantly higher than those in slow cooling group ( P0.05 ) .

同时，短期和长期热适应的细胞在复苏时，HSP基因的表达都呈指数衰减，但前者的衰减速率快于后者，这与热耐受动力学有明显相关性。

Meanwhile , expression of the HSP genes in the recovering cells after short - and long-term heat adaptation all decays exponentially with the former declines in a faster rate , which is tightly related to the thermo-tolerance kinetics .

【方法】P19细胞经复苏、培养、传代后，在体外二甲基亚砜（DMSO）诱导下向心肌细胞分化，在光镜、倒置显微镜下观察细胞形态学变化。

【 Methods 】 P19 cells were cultured and induced to differentiate to cardiac myocytes by 0.9 % dimethyl sulfoxide ( DMSO ) in vitro . Cell morphological changes were observed under the inverted microscope .

目前我们冻存细胞的复苏率为82.2%。

By now , the rate of frozen cells ' anabiosis was 82.2 % .

温度对冻存小鼠精原细胞的复苏效果

Recovery Effect of Temperature on Cryopreserved Mouse Spermatogonia

不同降温速率对脐血干细胞冷冻复苏后生物学特性的影响

Effects of Different Cooling Rates on Cryopreservation of Hematopoietic Stem Cells from Cord Blood

对已建株保存的3个民族的永生细胞进行复苏培养，复苏成活率为100%。

Culture recovery rate was 100 % .

采用不同温度对冻存后的小鼠精原细胞进行复苏，测定细胞复苏率。

Cryopreserved mouse spermatogonia are thawed at different temperature and the recovery rates are measured .

方法：1.DLBCL细胞的复苏与培养，观察细胞形态，了解生长情况和特性。

The DLBCL cells are reanimated , observe the proliferative status and morphological characteristic . 2 .

结论玻璃化冷冻能有效改善冻融卵母细胞的复苏及胚胎发育；

Conclusions : Vitrification could be more favorable than slow cooling in the survival and development of frozen - thawed oocytes .

不同冷冻速率在Vero细胞冻存复苏中的应用效果分析

Application effect analysis of the anabiosis in the different freezing speed to the vero cell

结果：烫伤延迟复苏后肝组织基因组DNA出现标志凋亡的梯形图象，发生凋亡的大鼠肝细胞随延迟复苏时间的延长而增加。

Results : The genomic DNA of the livers manifested the DNA ladder pattern , and the NO levels in the liver from burned rats with delayed fluid resuscitation significantly lowered when compared with burned rats with immediate fluid resuscitation ( P < 0 05 ) .

体外纯化培养的成熟星形胶质细胞经冷冻复苏方法处理后，诱导转化为放射状胶质细胞的类似细胞（RGLCs细胞）。

Purified mature astrocytes can be induced to transform into radial glia like cells ( named RGLCs ) in vitro .

大鼠胚胎神经干细胞的冷冻复苏

Cryopreservation of neural stem cells derived from embryonic rats

与新鲜细胞相比，复苏的内皮细胞活力保持在95%以上；

Compared with non-frozen endothelial cells , Post-thawed endothelial cells showed 95 % of vitality .

与此同时，将转基因细胞冷冻后复苏，再次测定细胞生长曲线。

At the same time another fibroblast cell growth curve was tested after the cells were frozen and thawed .

肝细胞冻存复苏成活率及胆红素代谢活性的实验评价

Experimental evaluation of the survival rate of resuscitation of adult mice hepatocytes after cryopreservation and its metabolic activity to human bilirubin