细胞分离技术

肝细胞分离技术的逐渐成熟，使肝细胞移植（hepatocytetransplantation，HCT）成为可能。

With the development in isolation of hepatocyte , it became possible to put hepatocyte transplantation ( HCT ) in practice .

[结论]该研究显示，我们的细胞分离技术能快速分离培养出毛囊外根鞘细胞，并且这些细胞含有bulge干细胞。

In the new dermal layer , the matrix decreased 4 weeks after transplantation . [ Conclusion ] This study gives evidence that our isolation technique allows isolating and culturing of outer root sheath cells , which contain some bulge stem cells .

骨髓单个核细胞分离技术研究及临床应用

Study of the bone marrow mononuclear cell apheresis procedure and clinical applications

他引进了单细胞分离技术。

He introduced the practice of isolating single cells .

优化骨髓单个核细胞分离技术的分析

Optimization of bone marrow mononuclear cell apheresis procedure

马铃薯单细胞分离技术的研究

Techniques on isolation of single cells in potato

成年小鼠心肌细胞分离技术

Isolation of cardiomyocytes from the adult mouse heart

荧光激活细胞分离技术在角膜缘干细胞研究中的应用

Application of fluorescent-activated cell sorting ( FACS ) technique in the research of rabbit limbal stem cells

鉴于在免疫抑制剂、器官获取技术、胰岛细胞分离技术和胶原酶方面取得的进展，我们有理由相信胰岛细胞移植将成为治疗1型糖尿病的最佳选择之一。

The advances in immunosuppressive regimens , organ procurement techniques , isolation techniques , and availability of defined collagenase blends have contributed to the continuing promise of making islet cell transplantation the treatment of choice for type 1 diabetes mellitus .

从靶向药物载体技术，细胞分离技术，免疫分析，酶的吸附与固定作用和基因治疗几个方面简要分析磁性纳米材料在生物医学领域的应用及其发展过程中有待解决的问题。

The application of magnetic nano-material in the field of biomedicine is introduced from aspects of drug-target technology , cell separation , immunity analysis , absorption and immobility of enzyme and gene therapy and the problems are raised to be solved in its application .

豚鼠耳蜗单离Hensen细胞的分离技术及其钾通道

Isolation of Hensen Cells From the Guinea-Pig Cochlea and the Properties of Potassium Currents in Isolating Hensen Cells

原代肝细胞分离培养技术现状及展望

Recent advances in techniques for isolation and culture of primary hepatocytes

小鼠味蕾细胞分离纯化技术建立及应用研究

The Study of on Separation and Purification Technology of Taste Bud Cells of Mouse and Application

表皮细胞分离培养技术的第一步就是要从皮肤中分离基底层细胞。

Isolation and culture of Keratinocyte , it is the first step to isolate the epidermal basal layer cells .

胰岛素作用原理的研究&Ⅱ.简化的大鼠肝细胞膜分离技术

Studies on the mechanism of insulin action ⅱ . a simplified method for the preparation of rat liver cell membrane

山羊胚胎体外培养体系的优化以及胚胎干细胞分离培养技术的研究

Study on the Optimization of in Vitro Culture System of Embryos and Isolation and Generated Culture Technology of Embryo Stem Cells in Goats

随着外科手术、免疫抑制药物、器官和细胞分离保存技术及移植免疫学基础的迅速发展，器官移植已经成为治疗器官功能衰竭的有效手段，给很多身患不治之症的患者带来了新生曙光。

With the rapid development of the surgery , immunosuppressive drugs , organ preservation and cell separation techniques and immunological basis of transplantation , organ transplantation has become an effective treatment of organ failure and brought the new dawn to many terminally ill patients .

将ES细胞分离与克隆技术和分子生物学技术相结合就可将新的遗传物质导入家畜的生殖腺细胞。

The new genetic material can introduce into germ cell in farm animal by the combination of embryonic stem culture with molecular biological techniques .

ES细胞分离、克隆技术与遗传工程和胚胎工程相结合，对于阐明动物生长发育的基本规律、抢救和保护濒危动物遗传资源、建立先进的动物育种技术体系具有重大的理论意义和实践价值。

The ES cell separation and cloning technique combined with genetic modification and the embryo engineering showed promising theoretical and practical values in animal growth and development model , the protection of endangered species , and establishment of advanced animal breeding systems etc. .

一种高效经济的小鼠胰岛细胞分离纯化新技术

A Novel Technique of Pancreatic Islet Cell Isolation and Purification

树突状细胞体外分离扩增技术研究进展

Separation and amplification of dendritic cells in vitro

方法：应用人表皮细胞的分离、培养技术，并进行MTT检测、病理切片以及电镜检测等手段进行观察。

Method : Use tissue engineering technology to separate and culture human keratinocyte in vitro and observe them using MTT method and histological and electron microscopic methods .

人脐静脉内皮细胞分离与冻存技术及Gore-Tex人工血管内皮化

Endothelialization of Gore-Tex vascular graft by using cryopreserved human umbilical endothelial cells

方法采用肺动脉插管技术、循环内皮细胞（CEC）分离技术及血液流变学检测方法，直接监测ALI时肺循环血液、外周血液的血液流变学指标及CEC变化情况。

Method Pulmonary arterial intubation , circulating endothelial cells ( CEC ) isolation and hemorheology detection technique were used to observe the changes of CEC numbers and hemorheology in rat pulmonary vascular system during oleic acid-induced acute lung injury ( ALI ) .

哺乳动物上皮细胞抗菌肽分离纯化技术研究进展

Progress on Isolation and Purification of Epithelial Antibacterial Peptides in Mammals

经过40多年的研究，尤其是近10年来应用半透膜、中空纤维技术、肝细胞分离及培养等技术，人工肝支持系统得到了迅速发展，在动物实验及临床应用中取得了较好效果。

During the last 40 years , especially during the later 10 years , much progress has been made in artificial liver support system in experiment and clinical applications with the development of semipermeable membrane , capillary holly-fiber , hepatocyte cell separation and culture techniques .

方法：取妊娠14.5d的小鼠胎肝，制作细胞悬液，用单克隆免疫磁珠细胞分离技术分离Sca1+细胞，PCR鉴别Y染色体性别决定区域（Sry）基因序列；

Methods : Sca-1 + cells from the murine fetal liver of 14.5 d were separated by Magnetic cell sorting ( MACS ) . The sequence of the sex determination region of the Y chromosome was determined by PCR .

方法对88例孕妇外周血分别通过密度梯度离心法，流式细胞术（FCM）和荧光激活细胞分离技术（FACS）分离NRBCs。

Methods NRBCs were isolated from peripheral blood of 88 pregnant women by density gradient centrifugation , flow cytometry ( FCM ) and fluorescence activated cell sorter ( FACS ) respectively .

由于在体环境异常复杂，细胞生理或病理现象的研究仍依赖于细胞体外分离和培养技术，因此，构建细胞体外加载实验系统是目前研究细胞的力学响应机制的主要途径。

Because of the staggering complexity of the in vivo environment , studying cellular responses to mechanical stress and strain has relied heavily on the use of in vitro mechanical loading systems .