细胞化学染色

目的：将国际标准的、稳定可靠的七种酶及糖原细胞化学染色试剂研制为盒式成套试剂。

Objective : Eight enzyme cytochemical stain in international standard , stable , reliable will be researched and produced to kit .

目的：探讨细胞化学染色法对急性白血病（acuteleukemia，AL）诊断分型的价值。

Objective : To diagnose and classify acute leukemia ( AL ) by cytochemistry staining .

通过免疫细胞化学染色技术检测NF-κB胞核阳性的淋巴细胞。

The immunocytochemical staining technique was used to detect activation of NF - κ B in lymphocytes .

免疫细胞化学染色法观察药物对I、III型胶原蛋白表达的影响。

Type I and type III collagen were detected by immunocytochemical staining .

在对细胞化学染色和地高辛标记的降钙素DNA探针原位杂交实验中观察到：分泌降钙素的DNA分布在细胞浆中，细胞核无表达。

From staining of cells and digoxigenin labeled DNA probe , observed the calcitonin secretion is in the cytoplasm , the nucleus-free expression .

利用免疫细胞化学染色法检测胃癌细胞中突变P53、VEGF在处理前后的变化。

The positive expression of mutational P53 and VEGF was determined by immunocytochemistry ( ICC ) .

免疫细胞化学染色，WESTERNBLOTTING检测BMP-2表达的变化。

BMP 2 expression of bone marrow stromal cells was analyzed by using immunocytochemistry and Western blotting .

免疫细胞化学染色检测细胞增殖核抗原（PCNA）的表达；

The expression of proliferating cell nuclear antigen ( PCNA ) was assessed by immunocytochemistry method .

用免疫细胞化学染色图像分析法和蛋白质定量分析法（WESTERNBLOTTING）测定U937细胞中NFκB的活性；

The activity of NF κ B was evaluated by immunochemical staining and image analysis and Western blotting .

用γ氨基丁酸B受体1亚型（GABABR1）特异性抗体免疫细胞化学染色技术，研究了其在大鼠脑干运动核团内的定位分布。

The localization and distribution of γ - aminobutyric acid B receptor 1 subtype ( GABA BR1 ) in the rat brainstem motor nuclei was investigated by immunocytochemical staining technique .

用免疫细胞化学染色和逆转录多聚酶链反应（RTPCR）检测培养星形胶质细胞NFκB蛋白和基因的表达；

The immunocytochemical method and RT-PCR were used to measure the protein and gene expression of NF - κ B in cultured astrocytes .

免疫荧光和免疫细胞化学染色鉴定NSCs和神经细胞；

NSCs and nerve cells were identified by immunochemistry ;

免疫细胞化学染色示原代和传代细胞均为Nestin阳性。

Immunocytochemical method showed the primary and passage cells were nestin positive .

方法：用免疫细胞化学染色法检测HSC内增殖细胞核抗原（proliferatingcellnuclearantigen，PC-NA）的表达，流式细胞仪分析红景天甙作用于乙醛刺激的HSC后细胞周期的变化。

Method : Immunocytochemical stain was employed to detect the expression of proliferating cell nuclear antigen . ( PCNA ) in HSC .

结果：MSCs的CD44、CD54免疫细胞化学染色阳性率达90%以上。

Results : Positive expression was observed on more than 90 % MSCs .

用神经元特异性烯醇酶（NSE）抗体作免疫细胞化学染色，以便辨认神经细胞。

Neurons were identified by using the neuron specific enolase ( NSE ) immunocytochemical staining .

分别采用免疫细胞化学染色和Rl，一PCR检测BCSF刺激后脑微血管内皮细胞、人脐静脉内皮细胞ICAM一1蛋白及mRNA表达；

Immunocytochemistry and RT-PCR were used to observe the the expression of ICAM-1 protein and mRNA ;

免疫细胞化学染色法分析bcl-xL蛋白的表达；

The bcl-xL protein expression was detected by immunochemistry .

采用ABC免疫细胞化学染色法检测巨核细胞GATA-1和NF-E7的表达水平。

Immune cytochemistry was used to detect GATA - 1 and NF-E2 in megakaryocytes .

抗LH和FSH免疫细胞化学染色表明，大多数LH阳性细胞同时也是FSH阳性细胞。

Immunocytochemical study for LH and FSH showed that the majority of the LH positive cells were FSH positive as well .

Western-blot分析结果与免疫细胞化学染色结果一致。

The Western blot analysis had a results similar to that of immunocytochemical staining .

免疫细胞化学染色显示：CD14、CD45、胶原蛋白Ⅱ呈阴性；

The immunocytochemistry detection shows that CD14 , CD45 and collagen protein II are negative ;

以免疫细胞化学染色检测多药耐药基因（mdr1）的表达产物P-糖蛋白（P-gp）。

The expression of P - glycoprotein ( P-gp ) was detected by immunocytochemistry staining .

方法：采用免疫细胞化学染色法、免疫荧光激光共聚焦及RTPCR方法观察热体克蛋白（HSP）70的表达规律。

Methods : The law of expression of HSP 70 and its mRNA level were detected by immunocytochemical staining , laser confocal microscope and RT-PCR .

免疫细胞化学染色显示Pae作用后HT-29细胞bcl-2及p53蛋白表达显著降低。

Immunocytochemical staining showed that the expression of bcl-2 and p53 protein in HT-29 cells was decreased significantly after treated by Pae .

方法应用酶细胞化学染色法观察Lewis肺癌细胞酶活性的改变。

Method Enzyme cytochemistry was used to detect the activity of G-6-Pase and Mg2 + - ATPase of the mice lung cancer of Lewis cells .

免疫细胞化学染色观察CathepsinD在细胞内的表达及定位。

Immunohistochemical staining was used to observe the expression and location of cathepsin D within the JM cells .

实验从形态学角度表明：（1）NADPH-黄递酶细胞化学染色能够反映培养的大鼠肺泡巨噬细胞NOS表达状况；

The morphological results indicated that : ( 1 ) NADPH diaphorase cytochemical method could reflect NOS expression in cultured alveolar macrophage of rat in vitro ;

免疫细胞化学染色检测神经微管相关蛋白（MAP2）、乙胆碱酯酶（AchE）和酪氨酸羟化酶（TH）的表达，并计算阳性染色细胞的百分率。

MAP_2 , AchE and TH protein were detected using immunocytochemistry respectively and the percentage of positively stained cells was calculated .

结果免疫细胞化学染色发现HIF-1α蛋白在低氧培养A2780细胞核中表达呈阳性；

Results The immunocytochemical stain showed the positive expression of HIF-1 α protein in A2780 cell nucleus after hypoxic culture .