细胞同步化
- cell synchronization
![细胞同步化](../cache/1192695.jpg)
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方法:通过实验室常规分子生物学手段(细胞同步化、分子杂交&Northern杂交、Western杂交等)检测相关基因的表达变化。
Methods : Using the traditional molecular biology methods ( cell synchronization , molecular hybridization & Western blotting , Northern blotting , etc. ) examine the gene expression .
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方法复苏冻存的HK-2细胞,采用传代后的第2-5代细胞进行实验,进行实验前用无血清DMEM培养细胞12h使细胞同步化。利用密闭的细胞培养罐模拟缺氧环境,并检测其缺氧效果。
Passages 2-5 cells were used for the experiment after twenty-four hours cell synchronization in serum-free DMEM . A sealed cell culture vessel was used to induced hypoxia in our experiment .
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小麦根尖细胞同步化诱导和DNA纤维的制备
Synchronization of Meristem Division Induction and DNA Fiber Preparation of Wheat Root
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胚胎干细胞同步化处理、神经分化及Aβ对分化神经元NFκB通路的影响
Synchronization , Neuro-differentiation and Amyloid - β Peptides Inducing NF κ B Activation During Differentiation from Mouse Embryonic Stem Cells to Neuronal Cells in Vitro
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方法:运用血清饥饿法、化学药物双阻断法、有丝分裂药物阻断及摇落法分别将CNE-2Z细胞同步化至细胞周期的G1、S、M期,流式细胞仪检测其同步化效果。
METHODS : Synchronous cells were obtained by the serum deprivation , double chemical-block , mitotic arrest and shake-off techniques . Cell cycle distribution of CNE-2Z cells was analyzed by the flow cytometry .
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小鼠腹水瘤S(180)-V细胞同步化的研究
A study on the synchronization of mouse sarcoma cells
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红豆杉细胞同步化的研究
Studies on synchronization of Taxus cells
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肿瘤坏死因子的细胞同步化作用对鼻咽癌放射敏感性的影响
Radiosensitivity of nasopharyngeal carcinoma in relation to cell cycle synchronization effect of tumor necrosis factor α
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结论TNFα可通过促进CNE-2Z-S1细胞周期同步化进而增加放射敏感性。
Conclusion TNF α can enhance the radiosensitivity of NPC cells by inducing cell cycle synchronization .
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利用加入氨甲喋呤和胸苷使细胞分裂同步化并结合胰酶G带技术,分析了猪前中期染色体高分辨G带。
Analysis of high resolution G bands of prometaphase chromosomes in pigs was carried out by cell synchronization using amethopterin and thymidine treatment combining GTG technique .
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用胸腺嘧啶核苷双阻断法实现Vero细胞的同步化生长
Synchronous Growth of Vero Cells with Double Thymidine Block
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方法采用细胞周期同步化方法,观察结直肠癌化疗主要药物5-FU对人结直肠癌细胞CA-ⅡG1/S期进程的影响,并探讨不同的区域化疗药物浓度与肿瘤杀伤作用的量效关系;
Methods The technic of cell cycle synchronization was used to observe the influence of 5 FU on G1 / S phase progress and cell cycle regulation in human colon cancer CA ⅱ cell line .
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悬浮培养红豆杉细胞分裂同步化诱导
Induction of Mitotic Synchronization in Suspension Culture of Taxus chinensis Cells
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蚕豆根尖细胞分裂同步化的方法
The Method of Cell Division Synchronization in the Root Tip of Vicia
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不同低温对大蒜根尖分生区细胞的同步化作用
Effect of low temperature on cell synchronized division in the root tip of
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细胞周期同步化逆转卵巢癌对紫杉醇耐药的研究
Research of cell cycle synchronization on reversing taxol resistance of epithelial ovarian cancer cell lines
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目的探讨血清饥饿法进行细胞周期同步化实验的影响因素。
Objective To determine the effective factors that involved in cell cycle synchronization by serum starvation .
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但是,细胞周期同步化被证明是对细胞状态的一种干扰,可能导致假象研究结果。
But the cell cycle synchronization has been proved to disturb the cell growing status and might lead to pseudo results .
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在确定性网络,耦合强度增加会使得具有不同节律或电活动的细胞形成同步化周期1节律。
Synchronized period 1 rhythms are simulated in the deterministic network whose cells exhibit different rhythms when the coupling strength is increased .
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研究结果给出了心肌细胞网络同步化周期一节律形成的动力学解释。这就从理论上解释了同步化周期节律的产生机制,有助于认识正常心脏的起搏节律。
The results provided theoretical interpretation of the synchronized period 1 rhythm , helpful to recognize the normal pace-making rhythm of heart .
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对人宫颈癌细胞Hela进行同步化处理,收集并接触到Rose小室中。
Human cervix Hela cells were synchronized , collected and seeded into Rose chambers for experiment .
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而当振子的行为或参数远离Hopf分岔时,心肌细胞网络为同步化的周期1节律或静息。
When the behavior or parameters of the oscillator is far from the Hopf bifurcation , the synchronized network of cardiac myocytes is period 1 rhythm or rest .
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流式细胞仪监测细胞同步化程度;
The synchronizing degree of cells was monitored by flow cytometry .
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高浓度有机氰废水可生化性调控研究水曲柳体细胞胚胎发生的同步化调控
Adjustment and Control on Biochemical Performance of High Concentration Organic Cyanide Wastewater
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在兵豆初生根原皮层组织中,邻近位置上的细胞的细胞周期同步化程度较高。大鼠皮层神经元原代培养方法的改进
However , the adjacent cells partially synchronously proceeded their cell cycle . Improvement for culture of primary neurons of newborn rat cortical tissue
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不平衡的染色体分离普遍存在于诱导出的同源四倍体中,这就是导致生存能力不同的异常配子体出现的主要原因。大蒜根尖细胞有丝分裂同步化诱导与中期染色体分离
Imbalance chromosomal disjunction was common in the induced autotetraploids , which mostly gave rise to aberrant gametes , with different viability . INDUCTION OF SYNCHRONY AND ISOLATION OF METAPHASE CHROMSOMES FROM MITOTIC ROOT TIP CELLS IN ALLIUM SATIVUM
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并在对数生长期收获细胞,使细胞达到同步化后加入不同的实验处理因素。
Cell were collected in exponential growth stage at the same condition , and treated with different factors . 2 .
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以前,人们研究从单细胞到细胞团的同步化形成过程都是在大的网络中观察几个细胞从不同步搏动到同步化搏动的。
The procedure of the formation of synchronized process from single cells to network were studied in several cells whose behaviors were changed from non-synchronized rhythms to synchronized rhythms in a large scale network in previous studies .
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分别采用低温和咖啡因对红豆杉(Taxuschinensis)细胞进行处理,诱导细胞分裂同步化。
Taxus chinensis cells were treated with low temperature and caffeine in order to induce their mitotic synchronization .
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人体肝癌细胞株(7402)的细胞同步化和中期染色体分离方法
Synchronization of human hepatoma cell strain ( 7402 ) and the isolation of metaphase chromosomes