接种密度

贴壁24h后去悬浮细胞、低接种密度传代培养可获增殖能力强、功能活跃的MSCs。

Discarding the suspending cells after 24h incubation and subculturing the cells with low seeding density were helpful for acquiring MSCs with improved reproductive capability and active function .

转染组转染之前通过预实验确定合适的细胞接种密度，转染最佳体系，G418筛选浓度。

In transfected group , before transfection , our preliminary experiment found the appropriate cell seeding density , the best transfection system , and the appropriate G418 selection concentration .

悬浮系在继代培养第4天分离的原生质体植板率最高，在液体培养基中原生质体接种密度以1.5×105个·mL－1最佳，植扳率可达到24.43%。

The optimum plating density of proto-plasts was 1.5 × 105 / mL with a plating efficiency of 24.43 % in liquid culture .

前言：目的：建立人M5型白血病细胞系SHI-1/SCID（重症联合免疫缺陷）小鼠模型，探讨白血病细胞的接种密度与SCID小鼠成瘤率及病理变化的关系。

AIM : To establish a model of human M5 leukemia and investigate the pathomorphological change in severe combined immunodeficient ( SCID ) mice after being transplanted with SHI-1 cells .

结果表明：一次性培养12d，藻细胞由最初的接种密度1.9×105/mL，9d后上升到最高值1.41×107/mL；

The result shows : cell density increased from 1.9 × 10 5 / mL to 1.41 × 10 7 / mL after 12 days ′ batch culture .

目的观察不同接种密度对骨髓间充质干细胞（MSCs）与冻干脱钙骨基质（FDBM）体外构建组织工程骨的影响，以选择适宜的接种密度。

Objective To observe the effects of different seeding densities on formatting tissue engineering bone in vitro with freeze-dried demineralized bone matrix ( FDBM ) and mesenchymal stem cells ( MSCs ) .

不同接种密度对人表皮细胞培养融合的实验研究

The research of human keratinocytes cultured in vitro at different seeding concentration

在不同接种密度下，克隆的生长速度不同，实验结果表明：分瓶数量越多，克隆总重量增长越多。

The growth speed of clone is different in various inoculating density .

不同接种密度体外构建组织工程骨的实验观察

Experimental study of formatting tissue engineering bone with different densities of seeding cells in vitro

并比较不同接种密度，不同传代时间对神经干细胞生长的影响。

The effects of feed density and passage time on the cell growth were investigated .

结论人的骨髓间充质干细胞体内成骨能力受初始细胞接种密度的影响。

Conclusion The osteogenic potential of human MSCs is affected by initial cell seeding density .

2较适宜的表皮细胞接种密度为2×10~6／75cm~2flask。

The proper seeding concentration of human keratinocytes was 2 + 106 / 75cm2 flask .

适宜的表皮细胞接种密度为2×106cell/75cm2培养瓶。

And the proper seeding concentration is 2 × 106 cell / 75cm2 flask in primary culture .

饲养层细胞的接种密度对分离培养胚胎干细胞的影响

Effect of densities of feeder layer for isolation and culturing of Kunming white mouse embryonic stem cells

采用不同培养容器和接种密度进行小麦花药培养，研究了小麦花约培养中的密度效应。

The effect of density of wheat anther culture was studied with diverse containers and cultivating densities .

昆明白小鼠胎鼠成纤维细胞不同接种密度对人胚胎干细胞的影响

Effect of mouse embryonic fibroblasts of Kunming white mice of different inoculating densities on human embryonic stem cells

继代周期以25-30天为宜，合适的接种密度为每瓶3-5株。

The period of subculture was suitable for 25-30 days , and the density of inoculation was 3-5 / bottle .

外植体的接种密度影响愈伤组织及不定芽的诱导，接种密度以外植体5～10个／瓶较为适宜。

The inoculation density of the explants affected the callus and buds induction five to ten explants per culture bottle was suitable .

两种接种密度骨髓基质干细胞复合β-TCP对兔腰椎横突间融合的效果

Spinal fusion of lumbar intertransverse process in rabbits using two different densities of autologous marrow mesenchymal stem cells combined with β - TCP

血管紧张素Ⅱ诱导人脂肪干细胞向心肌样细胞分化可能与细胞接种密度以及细胞培养的材质有重要关系。

Angiotensin ⅱ - induced human adipose stem cells differentiating into cardiomyocyte-like cells may be influenced by the cell plating density and cell culture materials .

取第5代对数生长期骨髓基质干细胞，用成骨诱导试验进行体外分化潜能鉴定。⑤取第5代对数生长期的骨髓基质干细胞，按常规方法测定最适细胞接种密度。

Cloned MSC of passage 5 at logarithmic phase were selected to identify in vitro differentiation potential by ossify induction test and detect the optimal inoculating density .

通过不同接种密度瞬时生长率比较，证明克隆密度越低，生长速度越快；分瓶数量越多，总重量增长越多。

The experiment indicated that the lower the density , the faster the growth , and the more the divided bottle number , the more the total weight gain .

培养脂肪基质细胞，原代细胞80%融合后分别以5000/cm2和1000/cm2的接种密度传代至第3代。

ADSCs were seeded at different densities ( 5 000 and 1 000 cells / cm2 ) and cultured to passage 3 after primary cells completed 80 % confluence .

研究种子细胞接种密度对体外构建组织工程化三维支气管模型的影响，并筛选出适宜体外构建支气管模型的细胞密度。

To research the impact of seeding cell density on tissue-engineered3D bronchial model in vitro , which is in order to screen the proper density to reconstruct bronchial model .

以每丛3株左右，每瓶接3丛的接种密度，表现出显著的群体效应，可提高分化率。

The reproduction rate was more than 2.36 . The culture density of about 3 cluster a bottle with 3 plants a cluster expressed obvious community effect and raised the differentiation rate .

原代角质形成细胞接种密度在1×105/cm~2～5×105/cm~2时，细胞贴壁好，增殖速度快，体外培养周期显著缩短。

When inoculation density for the initial passage keratinocyte was between 1 × 105 / cm2 and 5 × 105 / cm2 , cells anchored well , proliferated fast and cell culture cycle was shortened significantly .

实验结果表明：在低的接种密度下静态接种有较高的接种率（88·9%），但随着接种密度的增加接种率下降显著，细胞结团且分布不均匀；

The seeding efficiency was relatively high ( 88.9 % ) at low inoculation cell density , and decreased rapidly with the increasing of inoculation cell density in static seeding . Stirred seeding yielded the lowest seeding efficiency .

目的：以昆明白小鼠胎鼠成纤维细胞作为饲养细胞，观察其不同接种密度对人胚胎干细胞的克隆生长情况及分化率的影响。

AIM : To investigate the influences of different density of mouse embryonic fibroblast ( MEF ), from Kunming white foetus mice as feeder cell , on the growth and differentiation rate of human embryonic stem cell ( hESC ) .

首先在体外用离心沉淀法和高密度连续培养诱导方法成功构建出了小球状和片状软骨组织，并且进一步证实了细胞接种密度和动态应力培养环境对软骨形成和成熟的重要性。

Firstly , pellet and patch-like cartilage was reconstructed in vitro by centrifugalization and continuous culture and induction . It was convinced that cell seeding protocol and dynamic cultural environment were essential to the formation and maturation of the cartilage tissue .

获取双侧股骨骨髓细胞，细胞接种密度1.5×106/cm2，置入6孔及24孔培养板内，随机分组，每孔作为1个样本，每组为24个样本。

Bone marrow cells of the bilateral femur were obtained , with the inoculated density of 1.5 × 106 / cm2 , implanted into 6 wells and 24 wells cultured plate , randomized into groups , a well as a specimen and 24 specimens in each group .