粉尘螨

粉尘螨是人体螨病的主要病原螨之一。

Dermatophagoides farinae is one of the main pathogenic mites causing human acariasis .

变应性鼻炎组粉尘螨和屋尘螨分别为73.20%和71.36%，狗上皮为33.54%；

Positive rates of the dermatophagoides farinae and pteronyssinus was 73.20 % and 71.36 % .

结论：屋尘螨、粉尘螨是广州地区小儿荨麻疹和AD患者最常见的变应原。

Conclusion : Mites were common allergen in infants and children with urticaria and AD.

粉尘螨I、II类变应原的克隆、表达研究

Cloning and Expression of cDNA Coding for Group I and II Allergen of Dermatophagoides Farinae

粉尘螨cDNA文库的构建

Construction and primary characterization of the cDNA expression library for Dermatophagoides farinae

粉尘螨-壳聚糖疫苗治疗过敏性哮喘小鼠肝脏Bcl-2的表达

Expressing of Bcl-2 on Allergic Asthma Mice Liver in Murine Therapy with Der f-CS

粉尘螨Ⅰ类变应原的cDNA克隆测序及亚克隆

Cloning , Sequencing and Subcloning of cDNA Coding for Group ⅰ Allergen of Dermatophagoides farinae

利用套式PCR技术鉴别屋尘螨和粉尘螨主要变应原基因及其在尘螨疫苗研制中的应用

To distinguish allergen genes of Dermatophagoides pteronyssinus from Dermatophagoides farinae by nested PCR and its application for the vaccine manufacture

粉尘螨Ⅱ类抗原cDNA原核表达质粒的构建与表达

Construction and expression of prokaryotic expression plasmid of cDNA coding for group ⅱ allergen of Dermatophagoides farinae

毛细管电泳测定粉尘螨主要变应原Derf2含量

Determination of Major Allergens Der f2 of Dermatophagoides Farinae by Capillary Electrophoresis

粉尘螨Derf2变应原的分离纯化及其特征

Purification and Characterization of Der f2 Allergen in Dermatophagoides farinae

目的克隆表达粉尘螨3类变应原（Derf3）基因，并鉴定纯化蛋白的变应原性。

Objective To clone , express and identify Der f 3 gene .

摘要目的用聚合酶链反应（PCR）技术对粉尘螨和屋尘螨进行鉴别和检测。

Abstract Object To identify and detect Dermatophagoides farina ( D.f ) and Dermatophagoides pteronyssinu ( D.p ) by Polymerase Chain Reaction ( PCR ) technology .

ABC-ELISA检测血清粉尘螨特异性IgE

Detection of mite-specific IgE in sera of asthmatics by ABC-ELISA DUST EXPLOSION

华南地区粉尘螨主要变应原Derf2的cDNA克隆及序列分析

CDNA Cloning and Sequence Analysis of Major Allergen of Dermatophagoides farinae ( Der f 2 ) in South China

变应性鼻炎患者血清与鼻分泌物中粉尘螨sIgE的相关性研究

Study of the relationship between sIgE of dust mite in serum and nasal secretion of allergic rhinitis

粉尘螨（抗原）PLGA微球对致敏小鼠肺部变应性炎症的影响

The Effect of murine allergy on pulmonary inflammation sensitized by the encapsulated dermatophagoides farinae antigen in PLGA microspheres

结论构建了华南地区粉尘螨3类变应原的原核表达载体，并高效表达和纯化出具变应原性的Derf3重组蛋白。

Conclusion Der f 3 gene has been successfully cloned and its prokaryotic expression vector is constructed .

方法用随机引物PCR对粉尘螨和屋尘螨的DNA进行扩增，选择三个扩增条带进行测序和分析。

Methods A randomly primed PCR ( RP-PCR ) technology were used to amplify the DNA of D , f and D.p , three amplified bands were selected for DNA sequencing and analysis .

目的获得大量具有良好的IgE结合活性的DerfⅡ重组变应原，以促进粉尘螨变态反应性疾病的特异性诊断及治疗的研究。

Objective To obtain large amounts of recombinant Der f ⅱ protein and detect its reactivity to the sera from patients allergic to Dermatophagoides farinae .

粉尘螨第五组主要变应原（Derf5）的克隆表达、纯化及免疫原性鉴定

Cloning , Expression , and Purification of Dust Mite Der f5 and the Evaluation of Its Immunogenicity

结论：测定可疑患者血清中粉尘螨sIgE可较好反映局部过敏程度；

Conclusion : Dertermining the Dermatophagoides farinae sIgE in the serum can reflect the local condition of suspected patients fairly well .

方法采集48例肠螨病患者血清，以粉尘螨（成螨）体组织切片为抗原，建立间接荧光抗体试验检测血清螨特异性抗体IgG。

Methods Adult mite of dermatophagoides farinae were used as antigen , and IFAT was applied to detect mite-specific IgG in sera of 48 patients with intestinal acariasis .

本文用生物化学和免疫学的方法研究粉尘螨（Dermatophagoidesfarinae）的抗原成分及其活性。

The antigenic fractionations and activities of Dermatophagoides farinae were determined by biochemical and immunological methods .

结果用CEAD测定了标准品Derf2的系列浓度，绘制标准曲线，线性好。并测定了药厂生产的各批不同浓度的粉尘螨注射液的Derf2含量。

Results To detecting a series of concentrations of Der f2 standard and the Der f2 contents of each batch of injection of Dermatophagoides farinae from pharmaceutical factory using CE ? AD method .

离子交换介孔分子筛DEAE-SBA-15的制备及其用于分离纯化粉尘螨主要变应原Derf2

Preparation of Ion Exchange Mesoporous Sieve DEAE-SBA-15 and Its Application to Separation and Purification of Der f 2 Allergen in Dermatophagoides Farinae

本文采用醛化绵羊红细胞以1：800粉尘螨抗原制成致敏血球进行间接血凝试验（IHA）。

Indirect hemoagglutination assay ( IHA ) was given to examine the serum of patients with pulmonary acariasis by sensitive sheep red blood cells absorbed by Dermatophagoides farinae extract as antigen .

目的研究粉尘螨提取液（Dematophagoidesfarinaeextract，DFE）对Ana-1巨噬细胞表达TNFα及ICAM-1的影响，探讨粉尘螨在引发变态反应疾病过程的作用机制。

Objective To investigate effects of Dermatophagoides farinae extract ( DFE ) on expression of TNF - α and ICAM-1 on Ana-1 macrophages and explore the mechanism of Dermatophagoides farinae inducing allergic diseases .

粉尘螨浸液通过葡聚糖凝胶柱层析，测得洗脱液的光密度值有2个峰（SephadexG-50）或3个峰（SephadexG-200）。

It was found that the optical densities ( OD ) of the fractions had 2 peaks ( Sephadex G-50 ) or 3 peaks ( Sephadex G-200 ), when D. farinae extract passed through gel filtration .

方法采用酶免疫分析法对468例过敏性疾病患者血清过敏源特异性IgE抗体进行定性检测，并给予仅对尘螨过敏的患者粉尘螨注射液脱敏治疗。

Methods The specific IgE for food and inhalant allergens were detected serologically by ELISA in the sera from 468 cases with the hypersensitive disease . The dermatophagoides farinae injection was used to desensitize the patients who were sensitive only to dust ( powder ) mite .