突变率

同时应用DNA序列分析确定gsp癌基因突变率。

Mutation rate of gsp oncogene was identified by DNA sequential analysis .

这个数值可以作为由DNA编码区控制的密码子突变率的最低估计值。

This numerical value can act as the lowest estimator of the mutation rate of codons controlled by the coding DNA .

用变性梯度凝胶电泳分析PCR克隆的突变率

Analysis of mutant frequency of PCR cloning by denaturing gradient gel electrophoresis

P53基因是基因研究最为广泛深入的抑癌基因，是肿瘤突变率最高的基因，对药物敏感性影响研究国外已有报道。

P53 gene is a cancer-inhibitor studied widely with the highest mutate-rate .

结果p53基因第5~8外显子总突变率为48.94%（23/47）。

Results Mutation rate of p53 exons 5 ~ 8 was 48.94 % ( 23 / 47 ) .

突变率与血清丙氨酸转氨酶（ALT）水平无关。

There was no correlation between infection with precore mutation and ALT serum level .

大肠癌组织p53突变率为48%（27/56）。

The mutations of p53 gene were detected in 48 % ( 27 / 56 ) of colorectal carcinomas investigated .

人NSCLC组织中DAL-11810CT突变率为4.1%。

The mutation rate of DAL-1 1810 CT is 4.1 % in human NSCLC tissue .

由于线粒体基因组缺乏组蛋白的保护和有效的DNA修复系统以及长期处于氧化磷酸化产生的氧自由基的环境中，线粒体基因组具有较高的突变率，大概是核基因组的10倍。

Furthermore , mtDNA is known for having high acquired mutation rates . The mutation rate for mtDNA is around 10 times higher than that of nuclear genomic DNA .

组织粪便APC基因突变率相比差异无统计学意义（χ2检验P＞0.05）。

There was no difference between the positive rate of the tumor tissues and stools by chi-square test ( P ﹥ 0.05 ) .

大量的遗传学和分子生物学研究表明p53基因与肺癌的发生密切相关，在非小细胞肺癌中其突变率为47.8%，小细胞肺癌中其突变率为80%左右。

It has been proved that there is approximately 47.8 % in NSCLC and 80 % in SCLC with p53 gene mutation .

结直肠癌k-ras基因突变率及相关因素的研究

The Mutation Rate of k-ras and Related Factors in Colorectal Cancer

Ras是ras基因编码的小GTP结合蛋白，在人肿瘤细胞中的基因突变率大约为30%。

Ras is a small GTP binding protein mutated in approximately 30 % human cancer .

研究小组正通过DNA测序，以及计算突变率和模式的方法，来找出珊瑚礁寄居者们是如何存活下来、甚至大量繁衍生息的。

A research team is investigating how the coral colonies are able to survive - and even thrive - by sequencing their DNA as well as calculating mutation rates and patterns .

APC突变率在肠型胃癌显著高于弥漫型胃癌（P<0.05）。

APC mutations were associated with intestinal type of gastric cancer ( P < 0.05 ) .

烟草能显著地提高伤寒沙门氏菌突变率和CHO细胞集落形成率；

Tobacco increased significantly the mutation rate of Salmonella typhia and the formation rate of CHO colony .

在各基因中，3A基因突变率最高。

In each gene , the 3A gene mutation rates are the tallest .

方法应用PCR-SSCP技术检测62例胃癌手术患者门静脉血中p53和K-ras基因的突变率。

Methods p53 and K-ras gene mutation was detected with PCR-SSCP technology in 62 cases of gastric carcinoma .

p53基因的高突变率提示PSH可能具有潜在的恶性生物学行为。

The high mutation rate of p53 gene may indicate that PSH has potentially malignant biological behavior .

结果全组CKIT蛋白的阳性率为97.6%（80/82），CKIT基因突变率为41.5%（34/82）。

Results The positive c kit protein expression and c kit gene mutation rates were 97.6 % ( 80 / 82 ) and 41.5 % ( 34 / 82 ) .

[结论]宫颈癌组织中p53基因突变率较低，提示p53基因突变并非宫颈癌组织中p53蛋白异常表达的主要原因。

[ Conclusion ] Low frequency of p53 gene mutation in cervical cancer tissues suggests that p53 protein abnormal accumulation may not result from p53 gene mutation .

rrs基因突变率依次分别为0、12.5%、7.2%。

The mutation rate of gene rrs is 0,12.5 % , 7.2 % respectively .

在25例肺腺癌中，Kras基因突变率为48.0%，其它类型肺癌中突变率仅为8.8%，而14例良性组织中未检测出Kras基因突变。

The mutation rate of K ras gene was 48.0 % in adenocarcinomas . No K ras gene mutations was detected in the specimens of benign respiratory diseases .

用多核细胞法检测细胞次黄嘌呤鸟嘌呤核糖转移酶（HPRT）基因突变率；

Multinuclear cell assay was used to detect the frequency of the HPRT gene mutation ;

结果：所有肺癌组织中，p53突变率54%（34/63），Bcl-2蛋白表达率44.4%（28/63）。

Results : Mutation rate of p53 was 54 % ( 34 / 63 ) , Bcl - 2 protein expression rate was 44.4 % ( 28 / 63 ) .

吸烟肺癌患者p53突变率（48.7%，19/39）显著高于非吸烟肺癌患者（15%，3/20）（P<0.01）。

P53 mutation ( 48.7 % ) in smokers with lung cancer was significantly higher than that ( 15 % ) in nonsmokers with lung cancer ( P < 0.01 ) .

健康成人和放射工作者体细胞HPRT基因位点突变率的检测和比较

Detection and comparison of mutant frequency of somatic HPRT locus between healthy adults and radiation workers

而H-ras基因第12密码子的突变率仅3.57%；

The positive rate of the H ras oncogene mutation was only 3.57 % ;

单个细胞中mtDNA拷贝数远比核DNA多，而且线粒体基因组碱基突变率约是核DNA的10倍。

There is a higher DNA copy number of mtDNA per cell than nuclear DNA . In addition , the mitochondrial genome has a 10 times higher nucleotide mutation rate than the nuclear genome .

方法采用RT-PCR技术和聚合酶链式反应-单链构像多态性分析（PCR-SSCP）方法，对46例结直肠癌的新鲜癌组织进行P53、K-ras基因突变率和VEGF表达率的检测。

Method RT PCR and PCR SSCP were used to detect P53 , K ras gene and VEGF in primary foci of 46 cases with colorectal cancer .