突变子

p53基因突变子175H、248W和273H的定点突变及表达载体的构建

Site-directed mutagenesis and construction of expression vector containing human p53 mutants 175 H , 248 W and 273 H

lacⅠ靶基因突变子的一种正向选择系统的建立

Development of a selective system for lac ⅰ gene mutants

目的：建立一种能正向选择lacⅠ靶基因突变子的选择系统。

Objective : To develop a selective system for positive selection of lac ⅰ mutants .

该项研究，为从T-DNA的突变子中分离紫杉醇产生菌产紫杉醇的相关基因提供了一条可行途径，也为阐明紫杉醇产生菌的代谢途径以及最终构建高产基因工程菌株奠定了基础。

This study provided a feasible way to seperate taxol biosynthesis related genes for taxol-producing fungi from T-DNA mutants , and also laid solid foundation for clarifying taxol pathway in taxol-producing fungi and for finally constructing high-yield gene engineering strain .

CSF－1R激酶突变子的构建及其突变体在真核细胞的表达

Construction of CSF-1R Kinase Mutant and Expression of Several Mutants in Eukaryotic Cell

因此，有必要建立一种在有机相中高通量筛选耐热性突变子的方法体系，为脂肪酶在有机合成中的应用奠定基础。

Therefore , it is necessary to establish a high-throughput screening in the organic phase , thermal mutants of the methodology for lipase in organic synthesis basis .

经5代的继代培养后，94%的突变子具有稳定性（潮霉素抗性）。对突变子产色素和桔霉素能力的分析表明其分泌次生代谢产物的能力发生了较大变化。

94 % of mutants screened was fully stable after five rounds successive culture and showed resistance to hygromycin B The abilities to produce pigment and citrinin were changed greatly by UV-VIS and HPLC analysis .

RT-PCR表明引入的氨基酸突变在子代病毒中存在。

Amino acid substitutions were present in progeny virus by RT-PCR .

E.coli中alKA基因突变启动子及体外转录的研究

The promoter mutants and in vitro transcription of alka gene E.coli

人Aγ-珠蛋白基因-173T→C突变对启动子功能的影响

Functional Analysis of Human A γ globin Gene Promoter with - 173 T → C Mutation

血液系统肿瘤中WT1基因突变及启动子区域DNA甲基化的研究

Study on WT1 Gene Mutation and Its Promoter Region DNA Methylation Status in Hematology Neoplasms

PTEN肿瘤抑制因子在乳腺癌中虽常呈失表达，但并不是通过基因突变或促进子甲基化而实现的，因此其失表达的机制还不清楚。

Expression of the PTEN tumor suppressor is frequently lost in breast cancer in the absence of mutation or promoter methylation through as yet undetermined mechanisms .

用上述方法检测SLE患者和健康献血员MBL外显子1区的基因突变和启动子区H/L的基因多态性。

Using the above-mentioned assay to detect the mutation of exon 1 and polymorphisms of the promoter region in MBL gene in SLE patients and healthy blood donors .

BCMA基因5'上游序列定点删除突变对启动子活性的影响

Effect on the promoter activity of BCMA gene 5 ' - flanking region deletion mutation

结果52例Rb肿瘤中除可见多态性、同义突变及内含子序列的变异外，未检出p53基因有真正的点突变。

Results Only did the polymorphisms , silent mutations and variants in intron have been shown in p53 gene , no real p53 gene mutation was found in retinoblastoma no matter the tumor carried Rb gene mutation or not .

P15、P16基因在多种肿瘤发生中起重要作用，其失活机制主要是缺失、突变和启动子区甲基化。

The two genes play an important role in a variety of human tumors , and their inactivation mechanisms are loss 、 mutation and methylation of the promoter region .

周期性质量突变量子化谐振子的高阶压缩

High Order Squeezing of the Quantum Oscillator with Sudden Mass Changes

这样的大群体有利于检出稀有的突变或重组子。

These large populations facilitate the detection of rare mutational or recombinational events .

碱基突变与密码子使用频率

Base Mutation and the Usage Frequencies of Codons

口腔粘膜鳞状细胞癌发生发展过程中p53基因突变的外显子分布特征

The characteristics of distribution of mutational p53 gene exons during multistage human oral carcinogenesis

中国人中的一种新的G6PD基因突变&外显子ⅥcDNA592C→T

A novel point mutation in the G6PD gene among chinese ── an exon 6 cDNA 592 C to T transition

对来自航天诱变凤仙花突变体院-3的子代（SP2）的减数分裂行为进行了研究。

The meiotic behavior in SP2 generation from Impatiens balsamina Yuan-3 induced by space flight has been investigated in this study .

结果gsp癌基因鉴定显示，18例垂体腺瘤组织标本中6例gsp癌基因表达阳性，5例突变位于201密码子，1例位于207密码子。

Results Among 18 tissue amples of pituitary adenomas , 6 samples showed gsp oncogene positive , and mutational sites were detected at codon 201 in 5 samples and codon 207 in 1 sample .

目的：检测中国人群动脉粥样硬化（AS）患者和正常人的胆固醇酯转运蛋白（CETP）基因15外显子D442G突变和14内含子I14A突变。

Objectives : To examine the frequency of exon 15 missense mutation ( D442G ) and intron 14 splice defect ( I14A ) in cholesteryl ester transfer protein gene in Chinese atherosclerotic subjects .

体外转录活性检测的实验数据表明突变型-144A启动子较野生型-144C启动子具有更高的转录活性，可以促进靶基因的转录。

Experimental data of in vitro transcription activity showed that the mutant - 144A promoter had a high transcription activity compared to - 144C wild-type and caused more transcription of target genes .

第二，局域态密度在空气散射子界面处发生突变，空气散射子区域的局域态密度相对较大，这可由电位移矢量的连续性来理解。

The local density of states takes much larger values in the air cylinders areas and drops down at the boundary between the air cylinders and dielectric medium background , and this phenomenon is due to the continuity of electric displacement vector .

农杆菌介导的红曲菌T-DNA插入突变库的构建及色素突变子的性质分析

Construction of T-DNA insertional library of Monascus mediated by Agrobacterium tumefaciens and characteristic analysis of the color mutants

一步法定点突变技术快速构建bsh基因突变启动子

Construction of bsh Promoter Mutants by Rapid One-step Site-directed Mutagenesis

β地贫基因突变以点突变为主，双重基因突变子多为重型β地贫，亦见于少数轻型β地贫。

Most of dual gene mutations were thalassemia major , but also observed in a few of thalassemia minor cases .

方法：对原发性高血压伴胰岛素抵抗患者胰岛素受体基因酪氨酸激酶域外显子17、18基因突变进行检测，并对突变的外显子进行核苷酸序列分析。

Me thods : Using PCR SSCP , we detected the mutation of INSR tyrosine kinase domain exon 17 and exon 18 in 33 primary hypertension patients and 28 normal persons .