种子培养基

最适种子培养基为可溶性淀粉1%，牛肉浸膏+大豆蛋白胨3.0%和NaCl0.5%，培养24h后，B12菌量可以达到34.1×10~9CFU/ml。

The starch , beef and soybean peptone and NaCl in the optimum seed culture medium is 1 % , 3.0 % and 0.5 % , and the microbe number of B12 could reach 34.1 × 10 ~ 9CFU / ml after 24 hours ' fermentation .

较适合的种子培养基为牛肉膏大豆蛋白胨培养基；

The suitable seed culture medium was beef extract soya peptone medium ;

研究了所获菌株S3的摇瓶发酵条件，应用正交设计试验法优化种子培养基和培养条件。

The fermentation condition of strain S3 in shaking flask was studied . The seed medium and the culture conditions were optimized by orthogonal design experiments .

采用60Co正交设计的方法，选择最优种子培养基配方和发酵培养基配方，在此基础上进行UV、60Co等诱变，得突变株，其发酵水平较原始菌株提高了150%。

The mutative strain , after excerpted the optima formula of medium for seeds and fermentation with the method of orthogonal design , were mutagenesised by UV , 60 Co and so on .

对高产蛋白酶菌株UF366米曲霉的种子培养基、制曲培养基以及发酵条件进行研究。

The composition of both starter medium and koji making medium as well as fermentation conditions of Aspergillus oryzae UF366 for producing proteinase were studied .

成功地筛选出理想液体培养基1号、生产种子培养基2号；

The ideal liquid medium 1 # and cultivate medium 2 # were screened successfully .

应用单因素和正交设计试验法，确定了种子培养基的最佳组成和最佳培养条件。

The seed medium composition and culture conditions were optimized by single factor experiment and orthogonal experiment .

应用正交设计试验法优化种子培养基，并研究了种子培养条件；

The seed medium was optimized by orthogonal design experiment and the seed culture conditions were also studied .

利用正交实验方法优化液体种子培养基的配方，通过单因素实验研究了孢子接种龄和接种量对种子生长的影响。

The seed medium was optimized by orthogonal experiments ; the spore time and inoculum 's effect on seed was studied by single experiments .

该菌种在小麦种子培养基上培养以后，除麦角新碱外，根据薄层层离鉴定结果证明还产生三种麦角生物碱。

By thin layer chromatography , it has been shown that this strain , cultured on wheat seed medium , was able to produce three other ergot alkaloids besides ergometrine .

在培养过程中，种子培养基含有的新霉素可以保持生产过程质粒稳定，这可以大大减少新霉素用量。

The neomycin in the seed medium could hold the stability of pDC-TNF during culture process , so the quantity of neomycin used in fermentation culture could be reduced much .

种子培养基、碳源补加、蛋白质处理和混合菌种发酵对总高级醇和正丁醇的生成有较大的影响；

Besides , seed culture medium , the addition of carbon sources , protein treatment , and mixed microbial fermentation would influence greatly the formation of total higher alcohols and n-butyl alcohol .

应用正交设计和响应面法分别优化出种子培养基和发酵培养基的配比，并对种子培养条件和发酵条件进行了研究。

The proportions of seed medium and fermentation medium were optimized by orthogonal design experiment and response surface analysis of SAS software . The seed culture conditions and fermentation conditions were also studied .

脱脂乳+20%番茄汁+2%菊芋抽提液作为种子培养基产酸速度快，双歧杆菌活菌数高。

Several formula of seed mediums were compared , milk + 20 % tomato juice + 2 % extractions from Jerusalem artichoke can accelerate to produce acid , attain higher Bifidobacterium Longum number .

将耐红霉素3万μg/ml的变株进一步用长波紫外线诱变处理，在含有5%正丙醇的种子培养基中进行筛选，得到耐红霉素和正丙醇，活性提高19.1%的变株。

The mutant resistant 30,000 μ g / ml of erythromycin was further treated by near UV , the mutant showing 19.1 % higher activity was isolated in the medium containing 5 % n-propanol .

杨树人工种子置于培养基5d后，A、B、E处理区尚无变化。C、D处理区开始发芽。

After 5 days the seeds on mediums C and D began to germinate , but no changes on A , B , E mediums .

土霉素种子斜面培养基配方的均匀设计优选

Optimization on formula of seed medium of oxytetracycline by uniform design

希蒙得木种子在培养基上易萌发，获得无菌幼苗；

The asepsis seedling will be easy got when the seed of jojoba are planted in culture medium .

结果表明，适宜灵芝G5760菌株发酵液体种子的培养基配方为麦麸粉5%，黄豆饼粉3%，KH2PO40.10%，MgSO40.09%；

The results showed that the optimum medium for fermentation liquid seed culture was wheat bran powder 5 % , soybean cake powder 3 % , KH_2PO_4 0.10 % and MgSO_4 0.09 % .

对于种子合成培养基，虽然尝试了优化混合氨基酸和金属离子的浓度，并考察了添加核苷类物质的影响，但均无法满足菌体的生长需要。

For the seed culture , although we tried to optimize the concentration of amino acid mixtures and metal ions , as well as studied the effect nucleosides addition , we could not obtain a defined medium that can support enough cell growth .

本研究还以奥地利黑松种子在无菌培养基和营养土中培养的实生苗带子叶顶芽为外植体诱导丛生芽。

The study also used the plantlets of sterile seedlings and seedlings in soil to induce tufted buds .

对无菌条件下白及种子在不同培养基上的萌发及其幼苗形态发生进行了研究。

Seed germination and seedling morphogenesis of Bletilla striata on the different media under sterile conditions were studied .

人工种子在转换培养基上转化成苗后，移植至混合土中盆栽3个星期后幼苗成活率达80%以上。

The seedlings converted from artificial seeds had 80 % of emergence rate after transfering to pots filled with mixed soil after 3 weeks .

[3]运用神经网络并结合遗传算法优化出最佳种子和发酵培养基组成，并研究了TV230菌株的最佳发酵条件。

[ 3 ] The optimal seed and fermentation medium were obtained by using neural network and genetic algorithms , at the same time the best condition was studied .

5杂交获得的小粒种子在MS基本培养基上或在铺有湿滤纸的培养皿中均能萌发，前者的萌发率为45.56%，后者为31.10%，但后者的畸形苗相对较少，且操作简便。

The small seeds obtained in 4x × 2x could germinate in MS medium as well in wet filter paper , and the rates of germination and abnormal seedling were higher in the former than in the later .

然后又对种子培养条件（包括种子培养基组成、种子培养基初始pH、装瓶量和种龄）进行了优化，得到了适合工业化生产的种子培养基和培养条件。

Then , a seed medium and cultivation conditions suitable for industrial production were obtained after the optimization of components of seed medium and seed cultivation conditions ( including the pH , the filling volum of seed medium , seed cultivation time ) .

种子液制备方法对乙醇发酵影响显著，种子培养基中添加2%的菊粉有利于Y05诱导产酶。

Method for seed preparation had significant influence on the ethanol fermentation .