滞育

滞育家蝇幼虫氨基酸、SOD、CAT的变化

The Changes of Amino Acid , SOD and CAT of Musca domestica Larva during Diapause

应用5个重复序列引物，通过PCR扩增试验，研究了不同滞育状态麦红吸浆虫DNA的多态性。

The DNA polymorphism of wheat midge at different diapause stages were analyzed by the PCR technique with 5 repeat sequence primers .

e不同日龄滞育蛹和非滞育蛹的过冷却点和结冰点测定结果表明，在预蛹至5日龄蛹期，过冷却点和结冰点均逐渐下降。

The results showed that supercooling point and freezing point decreased from pre-pupae to 5_day pupae period .

一化性和二化性的二化螟越冬幼虫在滞育解除方面的差异仅表现在群体临界化蛹前期的长短上，一化性CC品系的群体临界化蛹前期显著长于二化性PL品系。

Difference of diapause termination between univoltine and bivoltine was only in the length of critical pupation time .

在特定的环境条件信息下，神经分泌细胞产生和分泌滞育激素（DH）。

Under specific enviromental information , neuroendocrine cells produce and secrete diapause hormone ( DH ) .

同期滞育的裸露幼虫SOD、CAT和POD酶活性较结茧幼虫略高，但二者的酶活性没有显著的差异性。

There was not a significant difference of SOD , POD and CAT activity between no-cocooned larvae and cocooned larvae at the same diapause stage .

越冬蛹在冬季低温下培养和在恒温25℃下培养，在滞育期间两者之间CAT活力达到显著差异；

The CAT activity of hibernating pupae held at winter low temperatures was significantly higher than that of hibernating pupae held at a constant temperature of 25 ℃ .

化蛹12h后的滞育蛹发育与脑无关。

The development of diapause pupae did not need brain in the mid or later development stage .

此前我们的研究结果表明，H2O2代谢与家蚕滞育调控密切相关。

In previous studies , we reported that metabolism of H2O2 is closely related to the regulation of diapause in bivoltine silkworm .

至受精后48h，诱导组滞育的原肠胚和担轮幼虫比例显著高于对照组。

The arrested gastrulae and trochophore were found significantly higher in treated groups than that in controls during 48h post-fertilization .

体外25℃家蚕卵酯酶A4的ATP酶活性变化及其与滞育性的关系

Changes of ATPase Activity for Esterase A4 in vitro 25 ℃ and Its Relationship With Diapause in Silkworm Eggs , Bombyx mori

光周期信息的积累存在一个阈值，即需要积累一定数量的长夜或短夜循环去诱导50%的个体发育或滞育；预培养天数均为4d；

The threshold of photoperiodic information accumulation , i.e. the required day number for making 50 % individuals response .

家蚕滞育诱导过程中近日节律基因period和timless的表达研究

Research on Expression of Circadian Rhythm Gene Period and Gene Timless in the Diapause-induce Process of Silkworm , Bombyx Mori

盐酸处理使蚕卵酯酶A4的ATPase活性峰提早出现的时间还反映了蚕卵的滞育发育时间。

Moreover , the time of ATPase activity peak appearance moved up by hydrochloric acid treatment interrelated to the diapause development of silkworm eggs .

家蚕滞育生物钟蛋白质EA4基因的cDNA克隆和序列分析

Cloning and Sequence Analysis of cDNA of Diapause Biological Clock Protein Esterase A4 of Bombyx mori

利用RTPCR法，在转录水平上对烟夜蛾滞育激素（diapausehormone，DH）基因的时空表达进行了研究。

By using RT-PCR , a study was carried out on the time and spacial order of the diapause hormone gene expression in oriental tobacco budworm on the transcription level .

说明DH在进化上比较特殊，因此研究DH，不仅有助于探明滞育机理，而且对DH的分子进化研究也有重要作用；对DH分子进化的研究可以反过来可以推动滞育机理的研究，两者相辅相成。

Therefore the studies on DH not only will help ascertain the mechanism of diapause , but also have great significance for the molecular evolutionary studies of DH .

4月、5月新孢囊内无J2，夏季滞育期间，孢囊内有J2，但不活动。

There were no J2s in new cysts in April and May . J2s existed in cysts during CCN diapause in summer and were inactive .

鉴于谷胱甘肽氧化还原循环在H2O2清除中的重要作用，我们进一步测定了家蚕不同滞育阶段谷胱甘肽氧化还原循环的变化特点。

As glutathione redox cycle plays an important role in the removal of H2O2 , we further examined changes in glutathione redox cycle of bivoltine silkworm at above five different phases of diapause process in the present study .

BmNPV和AcMNPVegt基因启动子特性及家蚕海藻糖酶基因启动子活性的滞育激素调节研究

Characterization of egt Gene Promoters from BmNPV and AcMNPV and Regulation of Diapause Hormone on Activity of Bombyx Mori Trehalase Gene Promoter

家蚕滞育生物钟是依赖卵内酯酶A4（EA4）的倒计时型生物钟。

The diapause biological clock protein of Bombyx silkworm is a count down clock which depends on esterase A4 ( EA4 ) in their eggs .

上述结果表明，不同催青条件显著改变了家蚕催青期和蛹期的H2O2代谢，滞育诱导阶段的家蚕H2O2代谢可能与滞育激素（DH）的表达密切相关。

The results showed that metabolism of hydrogen peroxide changed significantly in egg and pupae stage with the different treatments during incubation . Metabolism of hydrogen peroxide in the course of diapause induction may be related to the expression of diapause hormone .

在相同短日照（10h／d）下，较低温度（21℃以下）促进滞育，较高温度（25℃）抑制滞育。

Lower temperature ( below 21 ℃) promotes the incidence of diapause and higher temprature ( above 25 ℃) restrains it when they are subject under the photoperiod of 10 hours per day .

表明麦红吸浆虫的DH基因可能与其它昆虫的的同源性较差，根据目前已知其他昆虫滞育基因序列设计的引物很难扩增出麦红吸浆虫的滞育基因。

There maybe has a lower homology of the diapause hormone gene between WBM and other insects . So it was difficult to design primers according to current DH gene sequence from other insects so as to amplify DH gene of WBM .

7月2日、11日和21日采果者，幼虫脱果高峰和50%个体进入滞育出现的时间相互间均各推迟8d左右，说明提高果实成熟度可促进桃蛀果蛾幼虫的发育和滞育。

Both the peaks of larvae escaping off fruits and the dates of fifty percent larvae entering diapause were postponed by about 8 days within the fruits picked on 21,11 and 2 July , respectively .

自然越冬后，滞育蛹的存活率显著高于非滞育蛹，在土壤不同深处越冬的蛹的存活率存在差异，距土表15cm深的蛹存活率最高。

The survival rate of diapause pupae was significantly higher than that of non - diapause pupae after overwintering in the field , and survival rate of pupae varied with the depth of soil , among which the pupae in 15cm depth of soil had the highest survival rate .

6℃处理30天即可解除滞育。

A 6 ℃ treatment for 30 days could relieve diapause .

棉铃虫滞育蛹的抗寒力

Study on cold resistant ability of diapause pupae of cotton bollworm

家蚕卵中酯酶A4与滞育发育具有密切的关系。

Esterase A4 in diapause eggs works characteristically during diapause development .

滞育和非滞育棉铃虫脑的组织解剖学研究

The Brain Morphology of Diapause and Non Diapause Destined Helicoverpa armigera