比活力

所获得的牛脑CaM经过PDE（磷酸二脂酶）法测定分析，其比活力为10800IU/mg；

Observed by PDE method , specific activity of the CaM was 10800 IU / mg .

提纯倍数77.24，酶液比活力为16.22U／mg蛋白（对硝基苯磷酸二钠作底物）。

The purification multiple was 77.24 , and the specific activity was 16.22 unit / mg . Pr with pNPP as its substrate .

淀粉酶比活力呈“V”字型变化趋势，晚期活性较高；

Amylase specific activity changed as " V " pattern and reached high values in later stages .

运用浸提、超滤浓缩、硫酸铵分级盐析、透析脱盐、冷冻干燥，对纤溶酶进行提取和纯化，最终的比活力为1588.03U／mg，纯化了329.47倍，达到了电泳纯。

The enzyme was purified with ultrafiltration concentration and ammonium sulfate fractionation . The specific activity was 1588.03U/mg with 329.47 times purification .

精氨酸酯酶比活力20μmol/mg·min（底物TAME）。

The arginine esterase activity is about 20umol / mg min ( substrate TAME ) .

可见，从凝固鹅血中提取的SOD比活力高（纯度高、活性好），且提取过程不需加抗凝剂和分离血球，工序较简单。

Therefore , specific activity of SOD from coagulation goose blood is higher ( activity and purity is better ) .

从检测结果可以看出，DNA酶是本药物中含量最丰富的成分，其比活力达到16.14U/mg；

According to the results , the most abundant component is DNase , at the same time , several other enzymes are also contained in the preparation .

结果：在黄嘌呤氧化酶法反应体系中，SOD灭活后依然能够检测到SOD比活力值。

Results : In xanthine oxidase method , the specific activity of SOD still could be detected although SOD was inactivated .

在28℃下摇床180r／min培养30h，酶的比活力最高达到0.581mU／g。

The specific activity of the enzyme was 0.581 mU / g.

本文报道腺苷酸环化酶（AC）和环腺苷酸磷酸二酯酶（PDE）比活力的放射性同位素测定法。

A method was developed for determination of adenylate cyclase ( AC ) and cAMP phosphodiesterase ( PDE ) specific activities by radioisotopic assay .

结果表明盐析后可去除63.17%的杂蛋白，NK活力损失仅为10.15%，比活力为7753.90IU/mg。

It was shown that 63.17 % of the existing protein impurity could be removed and the specific activity was 753.90 IU / mg .

辛硫磷LD（10）剂量连续诱导棉铃虫4代，对AChE比活力和V（max）作用表现为先抑制后诱导增加，但变化幅度较小。

The specific activity and the Fmax value of AChE were increased after firstly and then decreased with induction of sublethal dose of phoxim over 4 generations .

经Bt预处理后，金龟子幼虫体内的乙酰胆碱酯酶的比活力与对照相比略有降低，谷胱甘肽-S-转移酶和羧酸酯酶的比活力均有不同程度的升高。

The activity of AchE in larvae was decreased slightly compared to the blank control and the activity of GsTs and CarE increased in some extent .

培养至15天，细胞内SOD的比活力为33.7U／mg蛋白，是天然贮藏蒜中的156%。

After culture for 15 days , the special activity of SOD in garlic callus is 33.7 U / mg protein , it is 1.56 times of that in nature storage garlic .

西洋参红果期过氧化物酶（POD）的比活力高低顺序为叶>茎>果肉>种子>根；

The active ratio of peroxidase ( POD ) of the red fruit , of American Ginsengwas in leaf . stem , flesh , seed and root order ;

对MPH纯酶的底物特异性研究中发现：它水解甲基对硫磷的比活力是水解乙基对硫磷比活力的20余倍。

We found that purified MPH have a 20-fold higher hydrolytic efficiency to methyl parathion than to parathion .

GOT、GPT比活力遗传力分别达87.44%、80.4%，可作为产丝量选择的重要生化指标。

The H of specific activities of GOT and GPT are 87.44 % and 80.4 % , respectively , which can be an effective biochemical criterium for selection of silk yielding .

肿瘤组织环磷酸腺苷代谢的研究Ⅱ.抗癌药物对肿瘤组织AC和PDE比活力的影响

Metabolism of the adenosine 3 ′, 5 ′ - cyclic phosphate in tumor ⅱ . effects of some antitumor drugs on the adenylate cyclase and camp phosphodiesterase specific activities in tumor

纯化的SOD比活力为8565.3U/mg，回收率为29.2%。

The specific activity of the purified enzyme was 8565.3U/mg protein , the activity recovery was 29.2 % .

以酪蛋白为底物，检测了该融合蛋白对papain的抑制比活力为40个抑制活力单位。

The inhibitory specific activity of rLyccys ( GST ) against papain with the substrate casein was detected to be 40 U / mg .

经优化后Cx酶比活力比优化前提高90.55U/ml。

The cellulose activity is 90.55U/ml higher than before through optimization .

经过纯化，酶的比活力达18620.20u／mg，纯化了5.23倍，酶活力回收率为27.54%。

The specific activity was 18620.20u / mg , 27.54 % of the alkaline protease was recovered with 5.23-fold purification .

SOD，APX，POD的比活力与鲜重之间有很强的相关性，相关系数分别为0.663,0.798和0.918。

The relative activities of SOD , APX and POD were closely related to fresh weight , with the correlation coefficient of 0.663 ,( 0.798 ) and 0.918 respectively .

采用氮蓝四唑光照法比较了几种虫草无性型及其相关真菌的SOD酶比活力及粗酶含量，同时应用聚丙烯酰胺凝胶电泳法进行了活性显示。

In this paper , the activity and crude content of SOD from several anamorphic strains of Cordyceps and its allies were compared by NBT-illumination method , and enzyme activity were showed by polyacrylamide gel electrophoresis .

纯化的藏系绵羊乳酸脱氢酶-A比活力为100.94U/mg蛋白，纯化倍数为12.0。经PAGE和SDS-PAGE分析均显示1条带。

The relative activity of purified LDH-A was 100.94 U / mg protein , with purification times of 12.0 . Only one band was observed when the purified LDH-A was separated with SDS-PAGE or native PAGE .

苯作业工人的PDE比活力较正常对照组高（P＜0.05）.而白血病组的PDE比活力与正常对照组比较差异无显著性（P＞0.05）；

The PDE specific activity in benzene operating workers was higher than that in control . but the PDE specific activity in patients with leukemia was no significantly different than that in control ( P 0.05 ) .

因此SOD，APX，POD的比活力可以作为评价植物产量的一条新途径，为优良品种的前期选育研究提供了新的思路。

It indicates that the relative activities of SOD , APX and POD can be used as a new approach to research the yield and breeding of superior variety of A. macrocephala .

2）与正常肝细胞比较，荷瘤小鼠肝细胞中乳酸脱氢酶、细胞色素氧化酶比活力显著升高（P＜0.01）。琥珀酸脱氢酶比活力无明显变化（P＞0.05）。

2 ) Compared with normal liver , the specific activity of lactate dehydrogenase and cytochrome oxydase on cancer bearing mice liver significantly rose up ( P < 0 . 01 ), but , the succinate dehydrogenase had no significant change ( P > 0 . 05 ) .

实验以酶活力、比活力、蛋白载量和活力回收为指标，探讨了给酶量、反应pH值、反应时间、离子强度等因素对固定化纤维素酶的影响。

The experiment carried the research on the effects of different coupling time , pH of buffer , the amount of cellulase and ionic strength on immobilized cellulase with four targets : activity , specific activity , protein binding capacity and activity recovery .

与GA交联法制备的固定化酶相比，具有良好的重复使用稳定性、温度稳定性和比活力。这是一种低成本、实用性强的脂肪酶固定化新方法。

Compared with the GA-immobilized lipase , the THP-immobilized lipase had higher reusability , thermal stability and activity than that immobilized by GA. This was a cheap and readily available new method of immobilization of lipase . 5 .