正义链

短发夹状RNA（ShorthairpinRNA，shRNA）由短的正义链、反义链（19-21nt）和连接它们的数个碱基组成，正义链、反义链呈反向重复序列，因其形似发夹而得名。

As one form of RNAi , short hairpin RNA ( shRNA ) is composed of both sense and antisense sequences with a few bases ( 19-21nt ) which are reverse repeat sequences named as loop between them .

在设计的靶序列5′端开始的19个核苷酸为正义链，中间加入9个核苷酸TTCAAGAGA的间隔以形成发夹结构，3′端加有转录终止信号TTTTTT。

The sense strand of siRNA was composed of the start 19 nucleotides of the 5 ' end of target sequence .

24只犬随机分为对照组和实验组（包括人工脑脊液组、正义链组和反义链组）。

Twenty four dogs were assigned to control group and experimental group , and experimental group was randomly divided into ACSF group , sense group and antisense group .

构建的双启动子载体中，U6和H1相对排列，均以其间插入的靶序列为模板，分别转录出其正义和反义链，形成功能性siRNA。

Upon transfection into mammalian cells , the sense and antisense strands of the duplex were transcribed by these two convergent promoters from the same template to form functional siRNA .