核酸原位杂交

方法EBER－1核酸原位杂交及LMP－1和p53免疫组织化学技术。

Methods EBER-1 in situ hybridization and immunohistochemistry with antibody to LMP-1 and p53 protein were applied .

以光敏生物素－金标链亲和素核酸原位杂交及免疫组化方法检测膀胱癌病理标本中P53基因片段及其蛋白表达。

With nucleic acid in situ hybridization and immunohistochemical methods , the p53 gene fragment and its protein expression were determined .

采用组织切片核酸原位杂交（Insituhybridization，ISH）技术检测各组术后各不同点PKCmRNA和NGFmRNA在坐骨神经表达的动态变化及轴突数目变化。

Nucleic acid in situ hybridization histochemistry technique and the computer imagine analysis were employed to detect dynamic changes of PKC mRNA and NGF mRNA in rat sciatic nerves .

方法：采用核酸原位杂交检测15例早期鼻咽癌活检组织中的EB病毒编码RNA（EBV-encodedRNA，EBER）。

METHODS : EBV-encoded RNA ( EBER ) in 15 cases of early-stage NPC biopsy tissue was detected by nucleic acid in situ hybridization .

方法：用SABC免疫组织化学法和地高辛精标记的ET-1cRNA探针核酸原位杂交组织化学法，观察了兔输卵管组织ET-1的分布和ET-1mRNA的表达。

METHODS : The distribution of endothelin-1 and the expression of ET-1 mRNA in the rabbits uterine tubes were studied using SABC immunohistochemistry and in situ hybridization histochemistry with digoxigenin-labelled rat ET-1 cRNA probe .

方法采用核酸原位杂交免疫组织化学技术（ISHH）检测大鼠轻、中度脑损伤大脑皮层和海马BDNFmRNA表达的动态变化。

Methods Nucleic acid in situ hybridization histochemistry technique with an RNA probe and the computer image analysis were employed to detect the dynamic changes of BDNF mRNA in the cortex and the hippocampus after mild to medium brain injury .

用地高辛配基（Dig－）标记我国肾综合征出血热（HFRS）病毒R22株M片段cDNAR3克隆探针，对HFRS病毒R22株感染的人血管内皮细胞（HECs）作核酸原位杂交。

RNA genome of R 22 strain of hemorrhagic fever with renal syndrome ( HFRS ) virus was detected in human vascular endothelial cells ( HVECs ) with in situ hybridization using Dig labelled R 3 cDNA probe .

在成模12周后，分别用光学显微镜、电子显微镜等方法观察肾脏形态变化，用免疫组织化学、核酸原位杂交和逆转录PCR方法检测DM大鼠肾髓质集合管AQP-2的表达。

STZ-induced DM rats were established for 12 weeks , then the tissue structure of the kidneys of both groups was studied under light microscope and transmission electron microscope . The expression of AQP-2 in collecting tubules of renal medulla was assayed by immunohistochemistry , hybridization in situ and RT-PCR .

石蜡包埋骨组织切片核酸原位杂交实验研究

Study of in Situ Hybridization in Paraffin-Embeded Bone Tissue Slides

免疫组化和核酸原位杂交在尖锐湿疣病理诊断中的意义

The Significance of Detecting Human Papillomavirus by Immunohistochemistry and in situ Hybridization in the Pathologic Diagnosis of Condyloma Acuminatum

用地高辛随机引物标记的人巨噬细胞炎性蛋白1α和血管细胞粘附分子1cDNA探针与各组内皮细胞进行核酸原位杂交。

The EC of all groups were hybridized , insitu , with the digoxigenin-labeled MIP-1 α and VCAM-1 cDNA probes .

这9个类型中有的结合细胞学镜检与核酸原位杂交技术已确定为异附加系。

Some of the 9 types have been identified as alien addition lines through cytology techniques and in situ hybridization of nuclear acids .

方法：应用地高辛标记的cRNA探针核酸分子原位杂交技术显示新生大鼠脑内转化生长因子β（transforminggrowthfactor-β，TGF-β）的mRNA表达。

METHODS : mRNA expression of transforming growth factor-beta ( TGF - β) in the neonatal rat brain was shown by in situ hybridization which labeled the cRNA probes with the digoxin .

方法采用核酸分子原位杂交，应用RNA探针对32例TCC组织与8例正常组织的DDX24基因表达情况进行检测。

Methods The expression of DDX24 new gene was detected by using in sity hybridization with RNA primer in32 cases of TCC and8 cases of normal bladder tissue .

方法：应用核酸分子原位杂交（ISH）技术，对26例PCa和9例正常前列腺（NP）组织中PSCAmRNA进行检测和定位。

Methods : Using nucleic acid in situ hybridization ( ISH ) technique , the expression of PSCA mRNA in 26 cases of prostatic adenocarcinoma ( PCa ) and 9 cases of normal prostate ( NP ) specimens were observed .

用核酸分子原位杂交的方法，检测了WHV／myc转基因小鼠肝癌发生过程中c－jun、c－fos和c－H－ras基因的表达情况。

In situ hybridization was used with specific molecular probes to detect the expression of c jun , c fos and c H ras genes during hepatocarcinogenesis in WHV / myc transgenic mice .

目的探索适合肝穿标本石蜡切片核酸性原位杂交的方法。

To seek an effective method of nucleic acid in situ hybridization for paraffin sections of needle liver tissue .

方法：采用造血祖细胞体外培养、造血生长因子生物学活性检测、免疫细胞化学、核酸探针原位杂交等实验技术。

Methods : Techniques of hematopoietic progenitor cell culture in vitro , biological assay of hematopoietic growth factor ( HGF ), immunocytochemistry and in situ hybridization were used .