核固缩

超微结构的改变：SHR大脑皮质内神经元随着血压的升高出现核固缩、线粒体肿胀、嵴消失以及突触前后膜肿胀、突触间隙消失等改变。

Changes of the ultrastructure : The neurons in the cortex of SHR with the ascending of blood pressure , appeared karyopyknosis , mitochondria swollen , crista lost , the membranes of pre-synaptic and post-synaptic were not clear and the synaptic space was disappeared .

透射电镜观察显示，凋亡早期主要是线粒体结构的改变，后期则出现典型的凋亡征象，如核固缩、染色质凝集靠近核膜和凋亡小体形成。

The early apoptotic change under transmission electron microscope was mainly the structural changes of mitochondria , then the classical ultrastructural feature of apoptosis , such as karyopyknosis , chromatic agglutination adjacent to the nuclear membrane and apoptotic body were found in late stage .

荧光显微镜发现典型的核固缩及形态异常的有丝分裂中期细胞，DNA电泳发现典型的梯状DNA条带。

Nuclear condensation and abnormal metaphase cells were found by fluorescent microscopy . Typical DNA ladder was found by DNA electrophoresis .

IR组肝组织细胞表现为核固缩或破碎，嗜酸性增强，与周围细胞连接松散。

IR group , the liver cells showed nuclear condensation or fragmentation , eosinophils increased , loosely connected with the surrounding cells .

HE染色和荧光染色后，在光学、荧光显微镜下可见Q3、HeLa细胞出现核固缩、碎裂、浓染及颗粒块状荧光等细胞凋亡的形态学变化。

After HE 、 fluorescence staining , optics and fluorescence microscope showed that AP extract could induce apoptosis of Q3 and HeLa Cells .

TUNEL染色所见阳性凋亡细胞，其凋亡特征为胞体缩小、核固缩、核碎裂、凋亡小体形成。

The apoptotic features of apoptotic positive cells found in TUNEL staining were shrinking cell body , nuclear pyrosis , nuclear fragmentation and the formation apoptotic body .

另外，MI大鼠心肌组织切片观察到：心肌细胞基本结构紊乱，核固缩且染色加深，有的核甚至溶解消失。

In addition , we observed MI myocardial tissue sections and found that the basic structure of myocardial was disorder and nuclear become condensation and stained deepened and some nuclear melt away .

普鲁士蓝和腾氏蓝反应证实含铁化合物的沉积。皮层及杏仁体氯化亚铁注射部位、实验侧海马CA3区神经元减少，核固缩，胶质增生明显。

Deposition of iron-containing compounds into cortex and amygdaloid body were observed by Prussian blue and Turnbull blue stainings .

结果视网膜组织正常培养24h后，ISRN（RGCs和INL细胞）中可见少数细胞胞核固缩和坏死。

Results Some cells in ISRN ( include RGCs and INL cells ) showed pyknotic nuclei and necrosis after 24 h in control culture .

结果：铝过负荷致小鼠脑内MAO-B活力显著升高和CA1区出现明显神经元丢失及核固缩；

Results : Aluminum overload elevated MAO-B activity , caused karyopyknosis and loss of neurons in CA1 area of hippocampi , in dose-and time-dependent manners .

原位细胞凋亡检测可见细胞变小，核固缩为一个或多个染色质团块，与未经ASON转染的K562细胞株相比，经bcl2ASON转染的K562细胞株对青蒿琥酯的诱导凋亡作用更加明显；

TUNEL showed that K562 cells became smaller and the nuclei presented one or many condensed chromatin masses , compared to K562 cells which were not transfected with bcl-2 ASON , bcl-2 ASON transfected K562 cells were more sensitive to artesunate ;

结果：光镜结果表明，蛴螬（终浓度为4mg/ml）作用于MGC-803胃癌细胞24h后，可见胞核固缩、胞核碎裂、凋亡小体形成等凋亡形态学变化；

Results : The grub extract significantly inhibited the proliferation of gastric cancer cells in vitro , morphologic changes of apoptosis with chromatin margination , karyopyknosis , karyorrhexis , apoptotic body were found by the light scope .

结果显示，一次性大面积切痂术前1d烧伤血清可使体外培养的内皮细胞脱落，细胞变形，细胞收缩间隙增大，胞浆出现空泡，核固缩。

Results : After being incubated with burn serum which obtained from patient on 1d preoperation , the HUVECs were deformed showing shrinkage of the cells and formation of intercellular space , and small vacuoles appeared in cytoplasm with karyopyknosis .

介入治疗后9h在光镜下可见癌细胞出现结构松散，24h癌细胞出现核固缩，48h见明显坏死，16～21d残存的癌细胞出现形态异常。

Under microscope , the structure of cancer cell appeared to be more porous 9 hours later interventional treatment and karyopyknosis was found 24 hours later , then we found obvious necrosis after 48 hours . Paramorphia of the relic cancer cells were found in 16-21d .

2海马CA1区发生大量神经细胞核固缩的形态学改变，核固缩细胞数由对照组的（3.20±2.40）个/mm线长度增为（98.40±20.70）个/mm线长度（P<0.01）；

Pyknosis of the neuron nuclei in hippocampus CA 1 was widespread and the number of pyknosis per mm length was increased from 3 20 ± 2 40 in the control group to 98 40 ± 20 70 ( P < 0 01 );

胞质肿胀疏松，有的细胞出现核固缩。

Swelling and pyknosis in cytoplasm and karyopyknosis in some cells were found .

荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞；

Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration .

主要表现为组织炎性浸润，表皮组织增生，核固缩等。

The main manifestations were inflammatory infiltration , epidermal hyperplasia , nuclear condensation , etc.3 .

病变神经元体积缩小，胞质着色深，核固缩，神经元周围出现空隙。

The volume of Neuron decrease , Cytoplasm 's stain is dark , karyopyknosis are observed .

注意，表面的细胞极为扁平，核固缩深染。

Notice how flat the surface cells are and how dark and pyknotic their nuclei have become .

胞核固缩或肿胀，细胞外软骨基质增多、均匀。

Nucleus is condensed or swelling . The cartilage matrix outside the cells is uniform and increases a lot .

结果与对照组大鼠比较，实验组大鼠味腺腺泡细胞的核膜凹陷、核固缩；

Results Compared with the control group , in diabetes rats acinar cell nucleus appeared nucleolemma introcession and karyopycnosis ;

运用吖啶橙染色可以看到典型的凋亡细胞，核固缩、断裂。

By the staining of acridine orange , the quintessential apoptosis cells with nuclear condensation and flaw have been founded .

结论兔窦房结组织细胞数随年龄增加而逐渐减少，并且在老年兔组出现核固缩、裂解现象；

Conclusion Cells of rabbit SAN are decreasing with ageing and there is pyknosis and nuclei splitting in old group .

由于核固缩、核碎裂、核溶解早于细胞碎裂脱落，使细胞核的体密度和表面积密度明显减小。

The nuclear volume density and surface density decreased because the changes in the nucleus preceeded those in the cell body .

Ⅳ期见核固缩或破裂，细胞收缩成脂肪性碎屑，也可形成空陷窝或微瘢痕。

In Stage ⅲ the nuclear pycnosis , cells breaking into numerous fatty debris and forming a micro-scar or an empty lacuna .

脾脏内淋巴细胞肿胀，坏死（核固缩、核溶解），胞核和胞浆内细胞器溶解甚至消失；

It could be found in the spleen that swelling of lymphocytes , organelles in the cytoplasm and nucleus dissolved and even disappeared ;

倒置显微镜下可观察到部分凋亡细胞出现染色质凝集、核固缩、核碎裂等形态学改变。

Morphological changes including chromatic agglutination , karyopyknosis , and nuclear fragmentation could be observed in some of the cells under inverted microscope .

术后病理检查发现癌细胞均有不同程度的核固缩、碎裂、胞浆凝固、变性，细胞间质水肿、纤维增生、炎细胞浸润，血管内膜增厚、血栓形成。

Interstitial edema , infiltration of inflammatory cells , fibroelastosis , thickening of vascular intima , thrombosis and inflammation of vessels were also found .

2～4月后骨细胞数目减少，核固缩，空虚陷窝增多，牙周膜内小血管闭塞。

From 2 to 4 month afterwards , the number of osteocytes reduced and pyknosis , empty lacunae as well as obliteration of small vessels in periodontal membrane were commonly seen .