显微注射技术

首先利用受精卵原核的显微注射技术，将MR1基因显微注射至CD-1？

By microinjection techniques on germ nucleus of zygotes hMR-1 gene were injected into zygotes of CD-1 ?

制作转基因动物的显微注射技术

Technique of Microinjection for Making Transgenic Animals

本实验将1.3copy的D基因型HBV全基因组作为目的基因，采用显微注射技术将其注入小鼠受精卵细胞雄原核，然后将受精卵移植于受体假孕母鼠输卵管内，发育产生子代小鼠。

In our experiment , 1.3 copy HBV ( genotype D ) was used as target gene , which was transferred into male pronucleus of fertilized eggs , and then the DNA-injected fertilized eggs was transplanted into mouse oviduct under phantom pregnancy , which developed into off-spring .

卵母细胞浆内单精子显微注射技术的临床应用

Research on the clinical application of intracytoplasmic sperm injection

单精子卵母细胞浆内显微注射技术的临床应用研究及相关影响因素分析

Research on the Clinical Application of Intracytoplasmic Sperm Injection and Related Factors Analysis

显微注射技术在制备鱼类嵌合体和转基因海水鱼上的应用

Progress on the application of microinjection technique in fish cell transplantation and in transgenic marine fish

单精子显微注射技术

Technology of Intracytoplasmic Sperm Injection

采用显微注射技术，将人β-干扰素重组基因注射到家兔受精卵雄前核内，共注射受精卵265枚，获仔兔35只。

Hu IFN - β recombinant genes were microinjected into male pronuclei of fertilized ova of rabbits .

运用显微注射技术注射新鲜的蚊卵进行转基因蚊的制备。

Recombinant plasmid was injected into fresh mosquito eggs by microinjection and transgenic mosquitoes were produced . 7 .

显微注射技术本身固有的缺点在很大程度上限制了转基因动物的研究及应用。

To the great extent , the study and application of transgenic animal are restricted by the inherent limitation of pronuclear microinjection .

本研究采用单精子卵母细胞浆内显微注射技术治疗男性因素不育症、多次人工受精失败的不明原因不孕症以及前次受精失败患者。

The present study was conducted to apply ICSI for clinic treatments on male infertility , idiopathic infertility and in vitro fertilization failure .

建立了白消安腹腔注射法不育受体小鼠模型。3、成功运用输出小管显微注射技术完成精原干细胞移植。

Established the mouse infertility receptor model by intraperitoneal injection of busulfan.3.Successfully completed the spermatogonial stem cell transplantation by using the efferent ductules of testis microinjection . 4 .

显微注射技术对胚胎发育有着关键性的影响，如注射剂量的不同将直接影响到胚胎体外发育的存活率乃至影响整个转基因动物的成功率。

The technique of microinjection is significant to embryo 's development . For example , the different volume of microinjection will have direct effect on the in vitro survival rate of the embryos and even the successful rate of the transgenic animals .

为建立对HBsAg耐受的小鼠模型，利用显微注射转基因技术，构建了CMV启动子控制的HBsAg（ayw亚型）转基因小鼠。

To construct a murine model that is immunotolerant to HBsAg , we developed the HBsAg ( ayw subtype ) transgenic ( Tg ) mice with CMV promoter by microinjection .

线虫基因显微注射和整合技术&设备、操作与指南

Gene Microinjection and Integration in C. elegans : Equipments , Processes and Guidance

目的：应用经皮附睾、睾丸穿刺取精术（PETSA），结合单精子卵浆内显微注射（ICSI）技术治疗阻塞性无精子症。

Objective : To treat obstructive azoospermia by percutaneous epididymal , testicular sperm aspiration ( PETSA ) and intracytoplasmic sperm injection ( ICSI ) .

目的采用神经解剖学常用的荧光金（FG）标记逆向神经示踪法，结合胎儿外科显微手术和显微注射技术，探讨脊椎裂胎鼠支配肛提肌的脊髓运动神经元的发育特点。

Objective Combining the microsurgery and microinjection technique , we investigated the motor neuron development of the spinal cord in fetal rats with spina bifida occulta by injecting the retrograde tracer fluorogold ( FG ) into the muscle levator ani .