文昌鱼

研究兴趣：比较基因组与分子进化，动物遗传多样性与dna分子标记，文昌鱼。

Research interests : comparative genome and molecular evolution , animal genetic diversity and DNA molecular marker , amphioxus .

血管活性肠肽（VIP）在文昌鱼体内免疫组织化学定位的研究

Immunohistochemical localization of vasoactive intestinal polypeptide in amphioxus

不同海水盐度和pH对文昌鱼精子寿命的影响

Effects of different seawater salinity and pH on sperm lifespan of lancelet

由此认为文昌鱼神经胚cDNA文库确已建立。

The results showed that the cDNA library of Qingdao Amphioxus neurulae had constructed .

逆转录-PCR扩增文昌鱼LIM类同源框基因片段

Amplification of LIM class homeobox gene fragment of amphioxus by reverse transcription-prc

青岛文昌鱼NADH脱氢酶亚基I基因片段的克隆

Cloning of Qingdao amphioxus NADH dehydrogenase subunit 1 gene fragment

通过WESTERNBLOTTING技术发现文昌鱼肝盲囊存在一个类似鼠GHR的膜蛋白分子。

Western blotting revealed the presence of a membrane protein molecule similar to rat growth hormone receptor in the hepatic caecum .

青岛文昌鱼发育信号传导相关基因hedgehog的克隆

Cloning of Qingdao amphioxus hedgehog gene , a molecule involved in intercellular signaling of development

本文首次报道了17α-甲基睾酮（17α-methyltestosterone，17αMT）可激发文昌鱼精子发生。

The paper reports for the first time that 17 α - methyltestosterone ( 17 α MT ) may promote spermatogenesis of amphioxus .

青岛文昌鱼hedgehog基因的研究

Study on Qingdao amphioxus hedgehog gene

急性时相蛋白：抗凝血酶Ⅲ、血浆纤连蛋白、前白蛋白和白蛋白在文昌鱼中的组织定位以及LPS处理后浓度的变化

Acute Phase Proteins : Antithrombin ⅲ, Plasma Fibronectin , Transthyretin and Albumin , Their Locations in Amphioxus and the Changes of Their Concentrations after Treatment with LPS

文昌鱼和斑马鱼C1q、C3及G10的进化分析及基因表达的研究

Analysis of Phylogenesis and Expression Patterns of C1q , C3 and G10 in Amphioxus and Zebrafish

文昌鱼ACTIVIN基因编码一个410个氨基酸的前体蛋白，前体蛋白包括信号肽、N-末端结构域和C-末端结构域。

ACTIVIN gene encoded a 410-amino-acid proprotein . The proprotein contained signal domain , N-terminal domain and C-terminal domain .

文昌鱼cathepsin、β-MSP和allantoicase基因的特征、表达和进化研究

Characterization , Expression and Phylogenetic Analysis of Amphioxus Cathepsin , β - MSP and Allantoicase Genes

文昌鱼LH-RH的初步分离及其生物活性的测定

Preliminary isolation and determination of LH-RH and its biological activity in amphioxus

提取青岛文昌鱼18小时神经胚中期mRNA，以5′脱磷的NotⅠoligo（dT）18为引物，反转录合成cDNA。

MRNA isolated from Qingdao amphioxus neurulae of 18 hour period was reverse transcripted into cDNA using Not ⅰ - oligo ( dT ) 18 as a primer .

文昌鱼（Amphioxus）属于文昌鱼纲&头索动物亚门仅有的一个纲，现存动物仅29种左右。

Amphioxus is a species of Amphioxi , the only Class of Cephalochordata and has about 29 species alive now .

文昌鱼中最早发现的与Sox基因同源的基因是Amphisox1/2/3基因，该基因在佛罗里达文昌鱼中的表达与神经外胚层的发生和神经管的形成密切相关。

The expression of this gene in Branchiostoma floridae is closely related with the occurrence of outside nerve mesoderm and the formation of the neural tube .

文昌鱼ALPase底物特异性及其效应物对酶活力的影响

Substrate Specificity of Amphioxus ALPase and on the Influence of Some Effectors on the Enzyme

盐酸胍对文昌鱼Branchiostomabelcheri（Gray）酸性磷酸酯酶构象与活力的影响

Relation between comformation and catalytic activity of ACPase from Branchiostoma belcheri ( Gray ) in guanidine hydrochloride denaturation

本文还利用PCR方法从青岛文昌鱼基因组DNA中克隆MASP（甘露聚糖结合凝集素相关丝氨酸蛋白酶）基因片段。

The MASP ( mannose-binding lectin-associated serine protease ) gene has been cloned by the method of degenerative PCR and the fragment of the PCR product is 630 bps in length .

我们采用二轮PCR的方法对4个单拷贝的文昌鱼Pax基因进行筛选，共获得24个阳性克隆，平均每个基因对应6个克隆。

We further formulated the library into characteristic DNA pools for two-round PCR screening for 4 single copied Amphi-Pax genes and identified total of 26 positive clones with average of 6.5 clones for each gene .

青岛文昌鱼Sec61γ基因的克隆与同源性分析

Cloning and Homology Analysis of Sec61 γ Gene in Qingdao Amphioxus

整封原位杂交结果表明，文昌鱼和斑马鱼的MRLC基因的表达图式存在明显差异。

Whole-mount in situ hybridization results show that distinct expression pattern of MRLC occurs in amphioxus and zebrafish .

此外，本实验还运用免疫比浊法以绵羊抗人的补体3的抗血清检测了文昌鱼体液中的补体3浓度为1.17mg／ml。此浓度和人血清及狗血清中的C3浓度基本相当。

In addition , titration by turbidimetric immunoassay ( TIA ) using sheep anti-human C3 serum revealed that amphioxus humoral fluid contained C3-like component , and its concentration is about 1.17 mg / ml , which is comparable to C3 concentration in human or dog sera .

演绎的文昌鱼AGAT蛋白质由426个氨基酸组成，理论分子量为49.3654kDa，蛋白质的等电点为6.65。

The predicted protein of the amphioxus AGAT is 426 amino acids in length , with theoretical molecular weight of 49.3654 kDa and isoelectric point of 6.65 .

所有这些表明文昌鱼GST-1属于一类新的GST&rho类，并且其表达具有组织特异性。

All these indicate that the amphioxus glutathione S-transferase belongs to a novel rho-class of glutathione S-transferases with a tissue-specific expression pattern .

厦门文昌鱼自然保护区周围海域浮游植物的生态

Ecological studies of phytoplankton in waters around Xiamen Amphioxus Reserve Area

文昌鱼的实验室繁育及子二代获得

Laboratory Culturing and Acquirement of the Second Filial Generation of Amphioxus

文昌鱼到了性腺成熟后才开始进食动物性食物。

The feeding habits of amphioxus were similar between different genders .