工具酶

目的获得重组3C蛋白酶，开发一种新型的基因工程工具酶。

Objective To obtain human rhinovirus 3C protease as a novel tool enzyme for gene engineering .

拓朴异构酶（Topoisomerace，TOPO）是参与DNA调整、转录、复制、修复的重要工具酶。

Topoisomerace ( TOPO ) is the important tool enzyme with the attendance of regulation , transcription , replication and repair of DNA .

α-半乳糖苷酶是B→O血型改造研究中的关键工具酶。

Galactosidase is the key enzyme in the research of B to O blood conversion .

计算工具酶对噬菌体基因组DNA分子和质粒DNA分子的操作既是酶切连接机制的运用。

Computing tool enzymes manipulate phage genome and plasmid DNA based on this mechanism .

B→O血型转变工具酶α-半乳糖苷酶cDNA克隆及表达

Cloning and Expression of Alpha-galactosidase cDNA for Seroconversion from Group B to O

结论：为B→O血型改造后残留的工具酶的检测提供了有效的方法。

Conclusion : It provides an effective method to detect residual α - galactosidase in B → O blood conversion .

羧肽酶B是重组胰岛素生产中的一个必需工具酶，同时在蛋白质测序等领域中也得到广泛应用。

Carboxypeptidase B is a necessary enzyme especially in the processing of recombinant insulin and is often widely used in the sequencing of proteins .

建立了用单一工具酶（CK）测定红细胞中三磷酸腺苷（ATP）含量的比色测定法。

A single method for the determination of ATP content in blood red cell was introduced .

它们在用作RNA和DNA工具酶、基因分析和诊断手段以及基因治疗药物等方面的潜力引人注目。

They have great perspective to be used as RNA or DNA treating tools , gene analysis , gene diagnosis , and gene therapy .

MMR是DNA修复系统中重要的工具酶，其功能主要是修复错误配对的碱基。

MMR is an important enzyme and plays an important role in the DNA repair system .

α半乳糖苷酶因可水解人B型红细胞表面的α半乳糖残基，使B抗原结构变成O抗原结构，而成为B→O血型改造的工具酶。

α - Galactosidase could hydrolyze the α galactose residues on the surface of human blood type B erythrocyte , resulting in the conversion of the B antigen to O antigen .

方法采用改进的ThompsonCaM激活PDE间接测定法检测CaM，反应液中除去工具酶PDE及CaM标准液检测PDE，利用火焰原子吸收分光光度法检测Ca~（2+）。

Methods PDE indirect determine of improvement Thompson CaM , PDE and CaM were removed in the response solution and atom spectropho-tometry .

方法：血清学反应确定α半乳糖苷酶残酶量安全值，用SDSpage银染法和酶活性检测法测定通用型血中微量残留工具酶量。

Methods : The safe amount of α - galactosidase was determined by seroconversion reaction . The minimal amount of residual α - galactosidase was detected by SDS-PAGE silver staining combined with enzyme activity assay .

目的人鼻病毒3C蛋白酶具有酶切物异性强且在低温下仍保持较强酶活性的特点而被用作工具酶。

Objective Application of human rhinovirus 14 3C protease as a tool-enzyme for more stringent sequence specificity and efficient cleavage at low temperature .

L1连接核酶的发现不仅为生命起源RNA世界学说提供重要依据，同时人们可以对其进行改造，设计出新型的工具酶与核酶药物。

L1 ligase ribozyme is not only an important evidence to support the hypothesis that the life originates from " RNA World ", but also the template as the new tool enzymes and ribozyme drugs .

R酶和M酶已作为工具酶广泛用于现代分子生物学领域，同时，以它们为材料的理论性研究也在蛋白质&核酸专一性作用、原核基因表达调控及抗病毒机制等方面取得重要进展。

A large number of R and M emzymes have been used in the field of modern molecular biology , in the meantime , theoretical research on R-M themselves has also made important progress in protein-DNA interaction , regulation of gene expression , and mechanism of antivirus in procaryote .

肠激酶（Enterokinase，EK）是目前生物制药领域纯化重组蛋白产品时用于切割融合蛋白的首选工具酶之一。

Enterokinase ( EK ) is one of the most useful tools as a fusion protein cleavage regent for purification of recombinant protein production in biopharmaceutical industry .

综上所述，聚阿拉伯糖内切酶不仅是一种新型的工具酶，同时也为研究其他AG水解酶提供了比对依据，可以进一步应用于结核分枝杆菌细胞壁的结构分析及寻找抗结核药物作用的靶点。

In a word , endo-arabinase was not only a powerful tool but also a compare basis on the studied of other AG-degrading-enzyme . In addition , it could provide further insight into the complex structures of mycobacterial cell walls and exploitation of the targets of anti-TB drugs .

中表达得到了具有酶活性的融合3αHSD。这为利用金属螯和亲和层析纯化融合3αHSD，并进一步建立以其为工具酶的血清总胆汁酸酶循环测定方法奠定了基础。

This work laid good foundation for the purification of recombinant 3 α - HSD by metal chelate chromatography , and also for the construction of an enzymatic cycling method to measure serum total bile acids with recombinant 3 α - HSD as the tool enzyme .

葡萄糖氧化酶是测定血糖的关键工具酶。

Glucose Oxidase is the key enzyme instrument of blood-glucose determination .

逆转录酶已经成为基因克隆中重要的工具酶；

Reversed transcriptase has been widely used in gene cloning .

海藻糖对医用诊断工具酶活性保护研究

Study on stability of diagonostic tool enzymes under trehalose protection

黑木耳多糖经海洋细菌产生的特异性工具酶降解形成具有生物活性的黑木耳寡糖。

Auricularia auricula polysaccharides degenerated by specific enzymes from marine bacteria produced Auricularia auricula oligosaccharide with biological activity .

褐藻胶裂解酶能裂解褐藻胶制备寡糖，是一种重要的工具酶。

Alginate lyase can degrade alginates into oligosaccharides , and it 's a kind of important tool enzyme .

它不但与生物体许多疾病相关，而且是目前用于治疗肿瘤和癌症的一种重要的工具酶。

It is not only related to many diseases in organisms , but also used to treat tumors and cancer as an important tool enzyme .

切出的目标产品首位氨基酸完全忠实于天然蛋白，因此在基因工程制药领域已经成为融合表达时下游纯化的首选工具酶。

EK allows any downstream fusion target protein to retain its native amino-terminus , without leaving any unwanted amino acid residues on their amino termini .

该文简要介绍磷脂化合物分类、结构特点，及不同动物、植物、微生物来源工具酶分子结构和催化性质。

This work gives a brief introduction of the classification and structural characteristics of phospholipids , and the molecular structures and catalytic properties of the enzymes obtained from animals , plants , and microorganisms .

近年来随着糖生物学的发展，转糖基β-半乳糖苷酶逐渐成为糖类合成的重要工具酶，被用于合成各种糖基化合物，乳果糖、低聚半乳糖就是其中比较突出的两种。

In recent years , with the development of glycobiology ,β - galactosidase has become an important tool for saccharide synthesis , various glycoside compounds such as galactosyl-oligosaccharides and lactulose are synthesized by its function .

近年来发展起来的利用各种工具酶、纳米颗粒等手段的核酸信号放大检测技术为此提供了新的契机。

In recent years , the development of signal amplification detection technologies based on various enzymes and nanoparticles , offered a new opportunity to achieve simple , fast , and highly sensitive detection of nucleic acids .

1本实验首次以浓缩磷脂为底物，以磷脂酶A2为水解工具酶，采用静止反应方式，进行了酶改性研究。

The results are as follows : The natural soybean phospholipid was first selected as substrate and the phospholipase Aa as the hydrolysis tool enzyme . The enzyme-modified phospholipid was studied by the means of quiescence reaction .