分子杂交技术

  • 网络molecular hybridization;molecular hybridization technique
分子杂交技术分子杂交技术
  1. DNA分子杂交技术在乙肝(血源)疫苗质量监测中的应用

    Application of DNA Molecular Hybridization Technique in Quality Control of HBV Plasma Vaccine

  2. 用分子杂交技术对17株钩端螺旋体基因组DNA同源性的研究

    Homology Study of Leptospires by Molecular Hybridization

  3. 应用电镜原位分子杂交技术对腺病毒DNA在宿主细胞内进行定位

    Localization of Adenovirus DNA in its Host cell Using Electron Microscopic in Situ Hybridization

  4. 用分子杂交技术分析不同种株利什曼原虫动基体DNA同源性

    Analysis of the sequence homologies in K-DNA of different Leishmania isolates by DNA hybridization technique

  5. DNA分子杂交技术在基因工程人白细胞介素-2质量检测中的应用

    The Application of DNA Molecular Hybridization Technique in the Quality Control of Recombinant Human Interleukin-2

  6. 二种分子杂交技术定量检测临床标本中HBVdna的比较

    Comparison of two hybridization quantitative assays for detecting HBV DNA

  7. 电泳印迹和分子杂交技术在肝内HBVdna研究中的应用

    Applications of electrophoretic transfer and molecular hybridization in the study of HBV DNA in liver

  8. 应用核酸分子杂交技术检测心肌炎组织中的肠道病毒RNA

    Nucleic acid hybridization technique detection of enterovirus RNA in myocarditis

  9. 生物素标记HBV、HEVDNA探针原位分子杂交技术

    In situ hybridization technique of HBV , HEV DNA probe marked by avidin

  10. 采用分子杂交技术检测2例人原发性乳腺癌HER-2原癌基因DNA及RNA水平的改变。

    The changes of DNA and RNA level of HER-2 proto-oncogene in 2 cases of human primary breast cancer were examined using molecular hybridization technique .

  11. 分子杂交技术对外周血单个核细胞及肝细胞内HBV-DNA的初步研究

    Investigation of HBV-DNA in peripheral blood mononuclear cells and hepatocytes with molecular hybridization

  12. 原位核酸分子杂交技术检测宫颈鳞状上皮病变HPV感染

    Determination of HPV Genotypes with in Situ Hybridization Technic in Patients with Different Types of Cervical Squamous Epithelial Lesions

  13. 方法主要为PCR,TOPO-TA克隆和分子杂交技术。

    Methods Mainly by means of PCR , TOPO-TA cloning and molecular hybridization techniques .

  14. 用组化方法与Northern分子杂交技术分析测定了增龄鼠肌纤维横截面积(CAS)与肌凝蛋白重链ⅡB型蛋白的基因表达和运动后上述两个指标的变化。

    The histochemical method and Northern analysis were used to investigate the molecular mechanism of aging skeletal muscle atrophy and the influence of exercise on it .

  15. 实验通过原位分子杂交技术,检测人骨髓巨核细胞分化发育过程中神经生长因子(NGF)基因表达情况。

    The expression of nerve growth factor ( NGF ) gene during development of megalocaryocytes was studied by in situ hybridization .

  16. Digoxigenin标记核酸探针分子杂交技术探讨

    Research on hybridization techniques with nonradioactive - Digoxigenin labeled DNA hybridization Probe

  17. 利用核酸分子杂交技术研究其垂体阿黑皮源(POMC)基因表达的变化。

    The pituitary POMC gene expression was investigated by dot and Northern blot hybridization .

  18. 应用免疫组化及核酸分子杂交技术研究人肝纤维化中与细胞外基质(ECM)沉积有关的细胞。

    The cells which related to deposition of extracellular matrix ( ECM ) in human liver fibrogenesis were investigated with immunohistorhemistry ( IHC ) and nucleic acid hybridization .

  19. ERIC-PCR分子杂交技术分析大熊猫肠道菌群结构

    ERIC-PCR based fingerprinting and molecular hybridization to analyze the characteristics of intestinal microflora of giant panda

  20. 病毒的核酸检测技术发展非常迅速,主要有PCR技术、分子杂交技术、dsRNA技术、NASBA技术和基因芯片技术。

    Detection techniques based on nucleic acid for plant viruses , including of PCR , molecular hybridization , dsRNA , NASBA and DNA chip , were developed rapidly .

  21. 用核酸分子杂交技术研究多胺生物合成限速酶L-鸟氨酸脱羧酶(ODC)的不可逆抑制剂&二氟甲基鸟氨酸(DFMO)对HL-60细胞c-myc、c-fos、c-Ha-ras癌基因及ODC基因表达的影响。

    The effects of α - difluoromethylornithine ( DFMO ) on the expression of c-myc , c-fos , c-Ha-ras oncogenes and ODC gene of HL-60 cells were investigated .

  22. 方法应用分子杂交技术研究EGF对体外培养的牛眼小梁细胞c-fos基因表达的诱导和3H胸腺核苷酸(3H-thymidineincorparation,3H-TdR)掺入法观察细胞DNA的合成。

    Methods Molecular hybridization technique was used to investigate the effect of EGF on c fos expression and its induction of bovine trabecular cells in vitro , and 3H TdR incorporation method was applied to study the synthesis of DNA .

  23. 本文采用目前最灵敏的HBVDNA分子杂交技术,利用α~(32)P和生物素标记DNA做为探针进行乙型肝炎的研究。对230例HBsAg阳性的携带者进行检测HBVDNA阳性者占26.5%。

    The most sensitive technique of molecular hybridization and the probes labelled with alpha - ~ ( 32 ) P and biotin to detect HBV-DNA were used in the study of hepatitis B. Two hundred and thirty HBsAg carriers were examined and 26.5 percent of them were positive in HBV-DNA .

  24. 本实验首先采用免疫组化及原位分子杂交技术,观察了IGF-Ⅰ及其mRNA、IGF-ⅠR、PKC及ERK在喉癌组织中的表达,并初步分析了与喉癌细胞增殖、分化及转移的关系。

    In this study , Immunohistochemistry and in situ hybridization technology were first adopted to investigate the expression of IGF-1 and mRNA , IGF-IR , PKC and ERK in laryngeal carcinoma tissue , and analyses were made on the relationship between laryngeal carcinoma cell proliferation , differentiation and metastases .

  25. 方法利用免疫组织化学及原位分子杂交技术,对不同阶段(20周)CCl4诱导SD大鼠纤维化肝内胶原生成细胞Ⅰ、Ⅲ、Ⅳ型前胶原mRNA及其相应胶原蛋白的表达状况进行观察。

    Methods The expression of α 1 ( I ), α 1 ( III ), α 1 (ⅳ) procollagen mRNA and the relevant collagen proteins in collagen producing cells during CCl4-induced SD rat liver fibrogenesis ( 20 weeks ) were detected by using in situ hybridization and immunohistochemical techniques .

  26. HBV&DNA分子杂交技术的建立及其临床方面的应用

    The technique of HBV-DNA molecular hybridization and its application in clinical medicine

  27. 用核酸分子杂交技术产前诊断人巨细胞病毒感染

    Prenatal diagnosis of human cytomegalovirus infection by nucleic acid hybridization

  28. 电镜原位分子杂交技术及其应用

    The Electron Microscopic in situ Hybridization and Its Application

  29. 生物素标记核酸探针的分子杂交技术及其应用

    The Technique of Molecular Hybridization with Biotin Labelled Nucleic Acid Probe and its Applications

  30. 研究对象:人精原细胞瘤标本。方法:利用原位分子杂交技术、免疫组织化学技术和图像分析技术进行研究。

    Subject : Seminoma testis from male patientsMethods : In situ hybridization ( ISH )、 Immunohistochemistry ( IH ) and image analysis technology were used in our research .