分子杂交技术
- 网络molecular hybridization;molecular hybridization technique
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DNA分子杂交技术在乙肝(血源)疫苗质量监测中的应用
Application of DNA Molecular Hybridization Technique in Quality Control of HBV Plasma Vaccine
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用分子杂交技术对17株钩端螺旋体基因组DNA同源性的研究
Homology Study of Leptospires by Molecular Hybridization
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应用电镜原位分子杂交技术对腺病毒DNA在宿主细胞内进行定位
Localization of Adenovirus DNA in its Host cell Using Electron Microscopic in Situ Hybridization
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用分子杂交技术分析不同种株利什曼原虫动基体DNA同源性
Analysis of the sequence homologies in K-DNA of different Leishmania isolates by DNA hybridization technique
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DNA分子杂交技术在基因工程人白细胞介素-2质量检测中的应用
The Application of DNA Molecular Hybridization Technique in the Quality Control of Recombinant Human Interleukin-2
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二种分子杂交技术定量检测临床标本中HBVdna的比较
Comparison of two hybridization quantitative assays for detecting HBV DNA
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电泳印迹和分子杂交技术在肝内HBVdna研究中的应用
Applications of electrophoretic transfer and molecular hybridization in the study of HBV DNA in liver
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应用核酸分子杂交技术检测心肌炎组织中的肠道病毒RNA
Nucleic acid hybridization technique detection of enterovirus RNA in myocarditis
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生物素标记HBV、HEVDNA探针原位分子杂交技术
In situ hybridization technique of HBV , HEV DNA probe marked by avidin
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采用分子杂交技术检测2例人原发性乳腺癌HER-2原癌基因DNA及RNA水平的改变。
The changes of DNA and RNA level of HER-2 proto-oncogene in 2 cases of human primary breast cancer were examined using molecular hybridization technique .
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分子杂交技术对外周血单个核细胞及肝细胞内HBV-DNA的初步研究
Investigation of HBV-DNA in peripheral blood mononuclear cells and hepatocytes with molecular hybridization
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原位核酸分子杂交技术检测宫颈鳞状上皮病变HPV感染
Determination of HPV Genotypes with in Situ Hybridization Technic in Patients with Different Types of Cervical Squamous Epithelial Lesions
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方法主要为PCR,TOPO-TA克隆和分子杂交技术。
Methods Mainly by means of PCR , TOPO-TA cloning and molecular hybridization techniques .
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用组化方法与Northern分子杂交技术分析测定了增龄鼠肌纤维横截面积(CAS)与肌凝蛋白重链ⅡB型蛋白的基因表达和运动后上述两个指标的变化。
The histochemical method and Northern analysis were used to investigate the molecular mechanism of aging skeletal muscle atrophy and the influence of exercise on it .
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实验通过原位分子杂交技术,检测人骨髓巨核细胞分化发育过程中神经生长因子(NGF)基因表达情况。
The expression of nerve growth factor ( NGF ) gene during development of megalocaryocytes was studied by in situ hybridization .
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Digoxigenin标记核酸探针分子杂交技术探讨
Research on hybridization techniques with nonradioactive - Digoxigenin labeled DNA hybridization Probe
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利用核酸分子杂交技术研究其垂体阿黑皮源(POMC)基因表达的变化。
The pituitary POMC gene expression was investigated by dot and Northern blot hybridization .
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应用免疫组化及核酸分子杂交技术研究人肝纤维化中与细胞外基质(ECM)沉积有关的细胞。
The cells which related to deposition of extracellular matrix ( ECM ) in human liver fibrogenesis were investigated with immunohistorhemistry ( IHC ) and nucleic acid hybridization .
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ERIC-PCR分子杂交技术分析大熊猫肠道菌群结构
ERIC-PCR based fingerprinting and molecular hybridization to analyze the characteristics of intestinal microflora of giant panda
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病毒的核酸检测技术发展非常迅速,主要有PCR技术、分子杂交技术、dsRNA技术、NASBA技术和基因芯片技术。
Detection techniques based on nucleic acid for plant viruses , including of PCR , molecular hybridization , dsRNA , NASBA and DNA chip , were developed rapidly .
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用核酸分子杂交技术研究多胺生物合成限速酶L-鸟氨酸脱羧酶(ODC)的不可逆抑制剂&二氟甲基鸟氨酸(DFMO)对HL-60细胞c-myc、c-fos、c-Ha-ras癌基因及ODC基因表达的影响。
The effects of α - difluoromethylornithine ( DFMO ) on the expression of c-myc , c-fos , c-Ha-ras oncogenes and ODC gene of HL-60 cells were investigated .
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方法应用分子杂交技术研究EGF对体外培养的牛眼小梁细胞c-fos基因表达的诱导和3H胸腺核苷酸(3H-thymidineincorparation,3H-TdR)掺入法观察细胞DNA的合成。
Methods Molecular hybridization technique was used to investigate the effect of EGF on c fos expression and its induction of bovine trabecular cells in vitro , and 3H TdR incorporation method was applied to study the synthesis of DNA .
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本文采用目前最灵敏的HBVDNA分子杂交技术,利用α~(32)P和生物素标记DNA做为探针进行乙型肝炎的研究。对230例HBsAg阳性的携带者进行检测HBVDNA阳性者占26.5%。
The most sensitive technique of molecular hybridization and the probes labelled with alpha - ~ ( 32 ) P and biotin to detect HBV-DNA were used in the study of hepatitis B. Two hundred and thirty HBsAg carriers were examined and 26.5 percent of them were positive in HBV-DNA .
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本实验首先采用免疫组化及原位分子杂交技术,观察了IGF-Ⅰ及其mRNA、IGF-ⅠR、PKC及ERK在喉癌组织中的表达,并初步分析了与喉癌细胞增殖、分化及转移的关系。
In this study , Immunohistochemistry and in situ hybridization technology were first adopted to investigate the expression of IGF-1 and mRNA , IGF-IR , PKC and ERK in laryngeal carcinoma tissue , and analyses were made on the relationship between laryngeal carcinoma cell proliferation , differentiation and metastases .
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方法利用免疫组织化学及原位分子杂交技术,对不同阶段(20周)CCl4诱导SD大鼠纤维化肝内胶原生成细胞Ⅰ、Ⅲ、Ⅳ型前胶原mRNA及其相应胶原蛋白的表达状况进行观察。
Methods The expression of α 1 ( I ), α 1 ( III ), α 1 (ⅳ) procollagen mRNA and the relevant collagen proteins in collagen producing cells during CCl4-induced SD rat liver fibrogenesis ( 20 weeks ) were detected by using in situ hybridization and immunohistochemical techniques .
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HBV&DNA分子杂交技术的建立及其临床方面的应用
The technique of HBV-DNA molecular hybridization and its application in clinical medicine
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用核酸分子杂交技术产前诊断人巨细胞病毒感染
Prenatal diagnosis of human cytomegalovirus infection by nucleic acid hybridization
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电镜原位分子杂交技术及其应用
The Electron Microscopic in situ Hybridization and Its Application
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生物素标记核酸探针的分子杂交技术及其应用
The Technique of Molecular Hybridization with Biotin Labelled Nucleic Acid Probe and its Applications
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研究对象:人精原细胞瘤标本。方法:利用原位分子杂交技术、免疫组织化学技术和图像分析技术进行研究。
Subject : Seminoma testis from male patientsMethods : In situ hybridization ( ISH )、 Immunohistochemistry ( IH ) and image analysis technology were used in our research .