壁细胞

目的：探讨云母单体颗粒制剂对实验性萎缩性胃炎（AG）大鼠胃粘膜主细胞、壁细胞的保护作用。

Objective : To study the protective action of mica monomer granule preparation on the chief cell and parietal cell of gastric mucosa in experimental chronic atrophic gastritis ( CAG ) rats .

结论H2受体拮抗剂能够诱导壁细胞H2受体出现脱敏，质子泵抑制剂未出现耐受现象。

Conclusion : H_2 receptor antagonists induce the desensitization of the H2 receptor on the the rat gastric parietal cell , but proton pump inhibitors do not have tolerance .

贴壁细胞不添加CO2培养初步探索

Preliminary study on culturing adherent cells without adding CO_2

对链脲佐菌素诱导的糖尿病大鼠壁细胞和G细胞的体视学研究

A stereological study on the parietal cells and the gastrin cells of the streptozotocin induced diabetic rats

MTT实验观察贴壁细胞的增殖活性。

MTT assay was used to study the activity of proliferation .

壁细胞内亦可检测到GnRHmRNA杂交信号，信号物质位于细胞质，细胞核为阴性；

GnRH mRNA hybridized signals were also detected in cytoplasm with negative nuclei .

mmLDL与MCSF对动脉壁细胞胆固醇酯蓄积的协同作用

Synergism of mm-LDL and M-CSF on the Accumulation of Cholesteryl Ester in Aortic Cells

治疗组血管壁细胞增殖期细胞数明显低于对照组（P均＜0.001）。

It also showed that the number of proliferous cells of the arterial wall in the treatment group was significantly lower than that in the control group ( all P < 0.001 ) .

观测各组胃黏膜溃疡指数（UI）、胃液pH及壁细胞超微结构的动态变化。

The changes of gastric mucosal ulcer index ( UI ), pH of gastric juice and the ultrastructural changes of parietal cells were observed .

人脾贴壁细胞中TNFα转换酶cDNA的克隆以及LPS对其表达的影响

The Cloning of TNF - α Converting Enzyme cDNA and Effects of LPS on Its mRNA Expression in Human Spleen Adhension Cells

结果：培养第3d开始出现梭形贴壁细胞，约第7d出现团状聚集细胞集落；

Results After 3 days culture , attached spindle-like cells Appeared and a number of cell clusters appeared within 7 days .

结果：诱导培养5～7天后的非贴壁细胞具有典型的DCs形态，无明显的增殖趋势；

Results : After5to7days ' culture , the non-adherent cells had the typical morphology of dendritic cells , which had no significant proliferation trends .

结果：TGFα主要位于正常粘膜增殖带细胞的胞质内，同时在胃腺基底部、主细胞及壁细胞上亦有表达，其表达部位较广泛；

TGF α was expressed mainly in the regenerative zone of cytoplasm and weaker expression were found at the basal portions of the gastric glands .

贴壁细胞均匀分布，3～5d增殖迅速。

Adherent cells distributed evenly and proliferated quickly within 3 to 5 days .

GABA也可能直接激活壁细胞，促进胃酸分泌。

GABA also can directly promote parietal cells to secrete acid by combining with GABA receptors on the parietal cells .

结果：1，分离培养的贴壁细胞（P3）具有典型的成纤维细胞样形态和BMSCs细胞表面标志。

Results : 1 , The cultured BMSCs in P3 possess typical fibroblast-like morphology and cell surface antigen of BMSCs .

利用ABC法对胎儿骨髓细胞长期培养贴壁细胞层的6种凝集素受体的分布及动态变化进行定性分析。

The localizations and changes of6 lectin receptors of the adherent layer cell in long-term bone marrow cultures of human fetus were studied by ABC method .

长期培养的急性粒细胞白血病患者骨髓贴壁细胞中src原癌基因的高水平表达

High Expression Level of src Oncogene in the Adherent Layer Cells in Long-Term Bone Marrow Culture from Patients with Acute Myeloid Leukemia

第2章采用（31）~pNMR方法考察了贴壁细胞及血小板的含磷代谢物以及细胞内pH。

Chapter two : ( 31 ) ~ P NMR was used in the research of some cells and platelets samples for the determination of pH and the study of phosphorous metabolism .

从胃粘膜分离壁细胞，有益于深入探讨壁细胞的功能，阐明与HCl分泌相关的生理机制。

It 's very useful to study the function of parietal cells and to reveal the physiological mechanism of HCI secretion through isolation of parietal cells .

结论：三种H2受体拮抗剂对壁细胞H2受体脱敏的诱导能力存在一定的差别，其中法莫替丁最强，西咪替丁最弱。

CONCLUSION : Three H2 receptor antagonists can induce different desensitization of the H2 receptor , among which famotidine induces the strongest and cimetidine does the weakest .

原代培养5～7d后贴壁细胞呈梭形成纤维样细胞和圆形巨核细胞。

There were two types of adherent cells after 5 to 7 days primary culture , fibroblast-like cells and round megakaryocyte .

结果获得的壁细胞经吖啶橙（AcridineOrange，AO）鉴定纯度达80%以上，原代培养经HE染色可见壁细胞呈分散小组式生长，且生长状态良好。

RESULTS Identified by acridine orange ( AO ), the purity of isolated and cultured rat gastric parietal cells was over 80 % and they grew very well .

目的：研究3种H2受体拮抗剂对鼠胃粘膜壁细胞H2受体脱敏的诱导能力。

AIM : To investigate the differences of the desensitization of the histamine H2 receptor induced by three H2 receptor antagonists on the gastric parietal cells in rats .

在不同时间点收集贴壁细胞，以RT-PCR法检测细胞因子/受体，吸取细胞培养上清以ELISA法定量检测TNF-α。

Culture cells were harvested at different time points of incubation for the detection of cytokine / receptor mRNA expression by means of RT-PCR and culture supernatants were assayed for TNF-a production by ELISA .

在细胞水平上研究IL-1β对壁细胞的作用，和研究IL-1β对壁细胞K~+离子通道的调控作用。

To study the effect of IL 1B to parietal cells in the level of membrane and to study the regulation effect of EL 1B to potassium channel of parietal cells .

12h肠壁细胞、马氏管和卵巢均出现阳性反应，蚊围食膜开始出现，但不完整；

12 h , stronger reactions were distributing in the midgut wall , ovary , malpighian tubule , the peritrophic matrix ( PM ) began to appear ;

在M-CSF、IL-3培养基中培养贴壁细胞6天后，可观察到细胞呈圆形，集落成群生长，细胞趋向融合。

Adherent cells cultured in the presence of M-CSF and IL-3 for 6 days acquired a colony morphology , cells became confluent .

采用HE染色和免疫组化方法结合生物体视学技术，对链脲佐菌素诱导的糖尿病大鼠在胃底腺的壁细胞和幽门部胃粘膜的G细胞进行立体计量研究。

The stereological survey on the parietal cells in the gastric oxyntic glands and the gastrin cells in the gastric pyloric mucosa of the streptozotocin induced diabetic rats was performed using combined HE staining and gastrin 's immunocytochemistry by Weibel 's stereological methods .

脐血CD34+细胞经SCF和TPO刺激，扩增潜能较高，体外扩增后可以获得少量贴壁细胞，传代后不再贴壁生长。

CD34 + cells proliferation is high by SCF and TPO activated . CD34 + cells were able to generate very few adherent cells and not proliferate after passage .