基因扩增技术

本文采用多聚酶链反应的体外基因扩增技术，对70例曾有流产史的早期孕妇作绒毛PCR检查，结果有1例为阳性，阳性检测率为1.43%；

The results of PCR showed that the villus of one case has positive reaction from 70 samples of early pregnant women with abortion history , positive rate was 1.43 % ;

LAMP（Loop-mediatedIsothermalAmplification）法是一种新的基因扩增技术，具有简便、准确、灵敏、快速的特点；

LAMP ( Loop-mediated isothermal amplification ) is a new genic amplification technology . Merits of this kit is rapid , accurate , sensitive and convenient .

LAMP技术不能利用同一引物，检测具有单核苷酸差异的不同株型或同一株型的病原菌，而具有可区分它们的能力，这种结果与内引物在环媒恒温基因扩增技术中至关重要的作用有关。

LAMP technology can not use the same primers to detect a single nucleotide difference between the same or different plant type plant type of pathogen , and this results associated with the inner primer in the LAMP technology has played a vital role .

方法用套式基因扩增技术对3组共85例慢性乙型肝炎患者血清进行YMDD变异检测，检测结果经琼脂糖凝胶电泳判断。第1组：40例未经拉米夫定治疗，HBVdna5×105拷贝/ml；

Methods 85 serum samples from patients with chronic hepatitis B were divided in 3 groups : ( 1 ) 40 cases not treated with lamivudine , HBV DNA5 × 10 5 copies / ml.

石蜡包埋表皮组织中β-连环蛋白基因扩增技术的建立

Amplification of β - catenin gene in the paraffin-embedded epidermal tissue

体外基因扩增技术在乙型肝炎诊断中的应用

Application of polymerase chain reaction in the diagnosis of hepatitis B virus infection

基因扩增技术中抗污染的研究

Application of Anti - contamination in Gene Amplication Technique

环媒恒温基因扩增技术文献计量分析

Bibliometric Analysis of Loop-mediated Isothermal Amplification

而在生物技术中，基因扩增技术一直是科学研究和临床应用中最基础最重要的基本技术手段。

In biotechnology , gene amplification technology has been a scientific research and clinical applications , the most basic of the most important basic techniques .

用菌体脂肪酸分析和随机DNA基因扩增分析技术进行菌株间的同源性研究。

Study of homogeneity were carried through by Thalli fatty acid profile analysis and randomly amplified polymorphic DNA analysis .

本文阐述了PCR（基因扩增）技术的原理、基本操作方法，及其在兽医生物制品疫苗污染病毒检测中的应用。

This article expounds the principle of PCR technique , main methods of operations and its application to testing for virus pollution causing by veterinarian biological product .

方法用PCR-RFLP和等位基因特异性扩增技术，对150名广州地区汉族正常人的CYP1A1和CYP2E1基因多态性进行了检测，并与其他人群进行了比较。

Methods A total of 150 healthy Guangzhou Hans were studied with PCR RFLP and ASA techniques . Current results were compared with the data on other ethnic groups .

方法：以克隆性IgH和TCRγ基因重排分别作为B和T细胞淋巴瘤克隆基因标志，应用PCR基因扩增技术检测ML患者IBM。

Methods : Clonal IgH and TCR γ gene rearrangements served as gene marker of B and T-lymphoma respectively , and polymerase chain reaction ( PCR ) was utilized to detect IBM in patients with ML.