定点突变

结论通过对抗CD3scFv抗体基因进行定点突变，实现了该抗体片段的高表达；

Conclusion By site mutation , a high expression fragment m2 of anti CD 3 scFv antibody was obtained .

改进重叠延伸法引入DNA定点突变的新方法

A New Method of Site-directed Mutagenesis by Modifying Overlap Extension PCR

定点突变技术在DNA缺失改造上的应用

An Application of Oligonucleotide-Directed Mutagenesis on Making Deletion Mutation

PCR定点突变血红蛋白基因的克隆和筛选

Clone and Selection of Mutant Hemoglobin Gene with PCR Site-Directed Mutagenesis

定点突变提高青霉素G酰化酶的稳定性

Enhancing Penicillin G Acylase Stability by Site-directed Mutagenesis

用DREAM技术进行全长质粒快速定点突变

Rapid site-directed mutagenesis on full-length plasmid DNA by using designed restriction enzyme assisted mutagenesis

PCR技术对人IL-3cDNA进行定点突变的研究

PCR Site-Directed Mutagenesis of Human IL-3 cDNA in vitro

运用重组PCR技术对耐高温α-淀粉酶基因进行定点突变。

The gene was mutated by recombinant PCR .

在分子克隆中用PCR进行定点突变

Point mutations by PCR in Molecule cloning

利用PCR进行基因定点突变方法的改进

An improvement on site-directed mutagenesis using PCR

一种改进的快速PCR定点突变技术

A Technique of Improved Rapid Site-directed Mutagenesis

玉米叶绿体ATP合成酶ε亚基的定点突变

Site directed Mutagenesis of ε Subunit of ATP Synthase of Maize Chloroplast

p53基因突变子175H、248W和273H的定点突变及表达载体的构建

Site-directed mutagenesis and construction of expression vector containing human p53 mutants 175 H , 248 W and 273 H

定点突变提高IL-4免疫毒素对淋巴瘤的选择性和杀伤活性

Improvement of Selectivity and Cytotoxicity of IL-4 Immunotoxin on Lymphoma Through Site-directed Mutagenesis

定点突变的人胰岛素基因在CHO细胞表达的研究

A study on the expression of site-directed mutated human insulin gene in CHO cell

通过合成寡核苷酸和PCR技术将SCFcDNA中的原核稀有密码子定点突变为同义的高频密码子；

The prokaryotic rare codons in SCF cDNA sequence were mutated into high frequency synonymous codons by method of PCR .

在获得重组胰高血糖素基因工程菌基础上，利用定点突变技术改造其第21位氨基酸天冬氨酸为丙氨酸，并经DNA测序证明胰高血糖素基因发生了点突变。

Using recombinant glucagon expression system , the 21st aspartic acid was changed to be alanine by means of site directed mutagenesis . Glucagon gene mutation was testified by DNA sequencing .

~（21）Ala定点突变胰高血糖素及结构与功能变化

Changes of Glucagon Structure and Function by ~ ( 21 ) Ala Site-Directed Mutagenesis

与原始菌株的数据比对发现：此次定点突变菌株所产木聚糖酶的最适温度仍为70℃，最适pH为6.5，在不同pH条件下的失活曲线也没有发生改变。

Finally found that the xylanase from this site-specific mutagenesis strain also with optimal temperature 70 ℃ and optimal pH 6.5 , there is any change in the inactivation curve .

青霉素G酰化酶β亚基Ser~（579）、Arg~（580）的定点突变研究

Studies on the function of ser ~ ( 579 ) and arg ~ ( 580 ) in β - subunit of penicillin G acylase with the method of site-specific mutagenesis

结论：成功完成了ret基因的体外定点突变。

Conclusion : Site-directed mutation has been fulfilled successfully .

该方法能避免了因多轮PCR和合成长引物导致突变的可能性，可作为一种定点突变的替代方法。头顶较长；诚如有的学者所指出的？

This strategy reduces the possibility of unexpected mutations resulted from overlapping PCR and synthesis of long primers , and could serve as an option of site-directed mutagenesis .

方法应用计算机预测TCS分子上可能的抗原决定簇，并进行定点突变。

Methods Predict the potential antigenic determinant on TCS molecule by computer modeling and induce site-directed mutation .

利用定点突变技术将这两个位置的Asp和Leu分别突变为Cys。

Asp and Leu in the two positions were mutated to cysteines using site-directed mutagenesis technology .

薤白EPSP合酶基因cDNA定点突变、表达及草甘膦抗性的研究

Site-directed Mutation and Expression of EPSP Synthase Gene cDNA of Allium Macrostemon Bunge and Its Resistance to Glyphosate

通过重叠延伸PCR方法对其潜在的转录因子结合位点进行定点突变，检测各位点对FASN基因转录的调控作用。

The effect of transcriptional factor binding sites on promoter activity was detected via overlap extension PCR .

采用大引物方法，利用质粒多克隆位点两侧的普通测序引物作为旁侧引物，在单个PCR管内，经2个步骤共34个循环进行定点突变。

Using normal sequencing primers adjacent to multiple clone sites of plasmid as flanking primers , a novel megaprimer method was performed in one tube with two steps and34 cycles .

用双引物法对GI基因进行体外定点突变，构建了GI突变体G138P。

The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene .

通过某些药物的定点突变获得第二代新生物技术药物，如胰岛素、EPO和t-PA的突变体；

Site-directed mutagenesis of some biotech drugs to get second generation biomedicines , such as mutants of EPO , insulin and t-PA.

一步法定点突变技术快速构建bsh基因突变启动子

Construction of bsh Promoter Mutants by Rapid One-step Site-directed Mutagenesis