同源重组

利用Red同源重组系统进行牛β酪蛋白基因敲除

Knock out of bovine beta casein gene by homologous recombination

用Red介导的同源重组结合酶切改构质粒DNA

Reconstruction of plasmid DNA with Red-mediated homologous recombination combined with restriction digestion

同源重组在染色体DNA基因克隆中的应用方法

The Application of Homologous Recombination Method in Chromosomal Engineering

基于Red同源重组和高效自杀性载体系统构建肠炎沙门氏菌突变株方法的比较

Comparison of two methods for constructing Salmonella enteritidis mutants : Red recombination system and suicide vector system

利用酵母的同源重组系统将这26个PCR产物转入酵母，最终23个发生了重组；

Utilizing the yeast homologous recombination system , 23 recombinants were obtained ;

PCR扩增同源重组片段筛选转基因胚胎应用细菌质粒转化技术，将大肠杆菌K（？）

Identification of Transgenic Embryo Using Homologous Recombinant Fragments The recombinant plasmid of E.

用PCR和基因组Southern杂交鉴定基因打靶实验中同源重组事件

Application of PCR and genomic Southern blotting to molecular identification of homologous recombination events in gene targeting

利用同源重组构建人脑的酵母双杂交cDNA文库

Construction of Yeast Two Hybrid cDNA Library of Human Brain through Homologous Recombination

将线性化的打靶载体导入ES细胞后，能与靶位点有效地发生同源重组，获得了20个靶基因被灭活了的ES细胞克隆。

Twenty ES cell clones with HPRT gene inactivated were obtained .

利用Red系统对BAC进行同源重组

Homologous Recombination of BAC with Red System

伴随着分子生物学的发展，一种基于λ噬菌体Red重组酶的同源重组系统已应用于大肠杆菌基因工程研究。

With the development of molecular biology , an efficient bacteriophage λ based Escherichia coli homologous recombination system has been developed for genetic engineering .

在此基础上，我们对该方法进行了进一步拓展，发展出直接用PCR产物构建同源重组杆状病毒的方法，使重组病毒的构建更为便捷。

This method was further improved by constructing linear fragment with PCR products , which simplified the process significantly .

关中奶山羊BAC文库构建及其利用Red系统同源重组的研究

Research on Construction of BAC Library of Guanzhong Milk Goat and Homologous Recombination with Red System

细菌内同源重组法构建HBVs区和C区基因非复制型腺病毒载体及其体外表达

Construction of replication-deficient recombinant adenoviral vector carrying HBV S and C region gene by homologous recombination in bacteria and its expression in vitro

b和广东株N.犬冠状病毒南京株M基因的同源重组分析

The identity between Shandong N. Recombination Analysis of M Protein of a Canine Coronavirus Wild Strain Isolated From Nanjing City

Red同源重组技术具有同源序列短（40～60bp）、重组效率高的特点。

These Red proteins enable the recombination events between DNA species with as little as 40 ~ 60 bp of shared sequences to occur at high efficiency .

利用大肠杆菌内质粒间同源重组的方法，将狂犬病病毒G基因插入腺病毒基因组的E1基因区，构建了带有狂犬病病毒G基因的重组腺病毒质粒。

A recombinant plasmid comprising adenovirus genome with rabies virus glycoprotein gene in E1 region was constructed by homologous recombination .

目前已鉴定的这一基因家族的多数成员均参与几种重要的DNA修复途径，包括碱基切除修复、同源重组修复和非同源末端重接。

Most members of the XRCC genes family participated in several DNA repair pathways , including base excision repair , homologous recombination repair and non-homologous end joining .

针对不同的DNA损伤。细胞内有许多不同的修复方式对其进行修复，包括碱基切除修复、核苷酸切除修复、错配修复、同源重组修复等。

There are many DNA repair pathways to different DNA lesions , including base excision repair , nucleotide excision repair , mismatch repair and homologous recombination repair .

本文采用非复制型脓病毒载体，利用同源重组方法，获得表达G蛋白多肽的非复制型重组腺病毒。

We used the replication - defective adenovirus vector and the homologous recombination , so we obtained the recombinant adenovirus by carrying the G-protein polypeptide .

基因敲除技术是建立在同源重组（HomologousRecombination，HR）基础之上对基因组进行遗传修饰的一种较理想的方法。

Gene targeting is a useful protocol to modify the cell genome based on the homologous recombination .

目的利用细菌内同源重组法构建含12密码子点突变的Kras基因重组腺病毒。

Objective To construct the recombinant adenovirus of mutant k ras by using the method of homogenous recombination in bacteria .

利用同源重组技术敲除金黄色葡萄球菌临床分离株sarA基因

Deletion of sarA in a clinical isolates of Staphylococcus aureus via homologous recombination

对其后代进行了基因鉴定，Southern杂交的结果表明，gfp基因和人工合成丝心蛋白样基因都存在于家蚕基因组DNA中且发生了预期的同源重组事件。

The integration of gfp gene into genomic DNA of silkworm and the occurrence of expected homologous recombination event had been proved by Southern hybridization .

结果表明，可能存在转录后同源重组等转录后的事件，在蛇毒C型凝集素类蛋白的多样性上起着重要的作用。

The results suggested the existence of a post-transcriptional processing event that was essentially homologous recombination at the RNA level , which might play an important role in the diversity of snake venom CTL-like proteins .

人类细胞内存在着完整的修复系统，其中包括碱基切除修复系统（baseexcisionrepair，BER）和同源重组修复系统（HomologousRecombination，HR）。

There are complete repair system in normal cells , including base excision repair system ( BER ) and homologous recombination repair system ( HR ) .

利用插入卡那霉素抗性基因和同源重组手段，本文构建了豌豆根瘤菌的hurL基因突变株。

Viciae hurL gene was mutated by insertion of a kanamycin resistance cassette .

方法：应用同源重组的方法构建携带bFGF基因的腺病毒载体，体外转染HUVEC。

Methods : The adenovirus vector harboring human bFGF cDNA was constructed by homologous recombination technique and transfect HUVEC in vitro .

利用PCR反应得到了26个水稻蛋白激酶的编码序列；为了提高同源重组的效率，降低引物合成的成本，试验采用两次PCR方法使重组位点达到50bp，明显提高了转化效率；

And 26 encoding sequences of these kinases were obtained through two rounds of PCR , enhancing the efficiency of transformation ;

大肠杆菌的同源重组依靠内源性的RecA蛋白。

In Escherichia coli most recombinations depend on intracellular RecA protein .