同源重组法

方法：利用细菌内同源重组法快速构建复制缺陷型腺病毒AdEGFP/hVEGF165，经尾静脉注射给同基因骨髓移植BALB/c小鼠；

Method : By using homologous recombination in bacteria , the replicating deficiency adenovirus Ad-EGFP / hVEGF 165 was constructed and then injected into post-BMT BALB / c mice vial the tail vein .

方法利用Ad-Easy系统、细菌内同源重组法快速构建重组腺病毒Ad-GFP/SCL。

Methods The recombinant adenovirus Ad-GFP / SCL was rapidly constructed by using Ad-Easy system based on the homologous recombination in bacteria . Then , Cajal interstitial cells cultured in vitro were infected with 1.6 × 109 pfu of recombinant adenovirus .

细菌内同源重组法构建HBVs区和C区基因非复制型腺病毒载体及其体外表达

Construction of replication-deficient recombinant adenoviral vector carrying HBV S and C region gene by homologous recombination in bacteria and its expression in vitro

目的利用细菌内同源重组法构建含12密码子点突变的Kras基因重组腺病毒。

Objective To construct the recombinant adenovirus of mutant k ras by using the method of homogenous recombination in bacteria .

多杀菌素产生菌基因组重排育种研究细菌内同源重组法快速制备重组腺病毒

High efficient generation of recombinant adenovirus by bacterial intermolecular homologous recombination

同源重组法构建枯草芽孢杆菌核黄素操纵子突变株

Construction of Bacillus subtilis Riboflavin Operon Mutant by Homologous Recombination

用同源重组法修饰含人α类珠蛋白基因簇的细菌人工染色体

The Modification of a Bacterial Artifical Chromosome Containing Human α - globin Gene Cluster by Homologous Recombination

目的：采用细菌内同源重组法构建含人内皮抑素基因的重组腺病毒Ad-CMV-hEn。

Objective : To produce recombinant adenovirus via high efficient homologous recombination in bacteria with human endostatin as target gene .

结论：细菌内同源重组法是一种高效、简便、快捷的重组体腺病毒载体制备方法。

Conclusion : The method of homologous recombination in bacteria is a convenient and high efficient method to produce recombinant adenovirus .