切方法

质粒DNA提取与酶切方法的比较研究

Methods Research of Distilling and Enzyme - cut Plasmid DNA

H型钢优化锯切方法研究

Research on optimized cutting of h - section

铁塔加工中的一种角钢零件优化下料方法H型钢优化锯切方法研究

Optimized Cutting Method for Angle Pieces in Fabrication of Towers RESEARCH ON OPTIMIZED CUTTING OF H-SECTION

3对蛋白质的胶上酶切方法进行了考察，通过对SDS一PAGE分离胶上蛋白质进行还原、烷基化等处理，改变酶切条件，得到一种适用于sDS一PAGE分离蛋白质的酶切方法。

Iii. An optimal in-gel digestion procedure for SDS-PAGE separated proteins was acquired via additional reduction and alkylation of the protein in gel slice .

方法：应用多聚酶链反应（PCR）结合限制性酶切方法对105例健康体检者与102例原发性高血压（EH）患者进行基因突变的检测。

Methods : Polymerase chain reaction combined with restrict enzyme digestion was used to detect the target gene variation in 105 normotensive controls and 102 hypertensive patients .

目的：用Red介导的单链寡核苷酸体内同源重组结合限制酶切方法对pBR322-Red质粒中的DNA序列进行精确的缺失突变。

Objective : To precisely delete a 2 100 bp DNA sequence in pBR322-Red plasmid using Red mediated single-stranded oligonucleotides in vivo homologous recombination combined with restriction digestion strategy .

目的应用酶切方法对中国人肝豆状核变性患者ATP7B基因中的高频突变点进行检测，并对家系进行基因诊断分析。

Objective To find high frequency mutation point in Chinese patients with Wilson disease and make the genetic diagnosis .

构建能在真核细胞中表达的靶向瘦素基因的siRNAs重组质粒，用酶切方法和测序法对重组体进行鉴定。

The recombinant leptin siRNAs plasmid vectors could be expressed in eukaryocyte , and then to evaluated them by using enzyme cutting and sequencing .

结论：由于PCR-双酶切方法快速、简单、易操作，且特异性好，可作为CMT1A基因诊断的一种初筛方法。

CONCLUSION : Because of the speediness , simpleness and good specificity , the PCR combined with restriction enzyme digestion can be used as a primary screening in the gene diagnosis of CMT1A .

我们应用&切方法去阻止流行病的传播。

We must try all means to block the spread of the epidemic .

并联电容补偿无功电流的模糊控制投切方法

Reactive current compensation of fuzzy controlling parallel capacitor switching

薄壁尼龙零件常规铣切方法研究

Study on Conventional Technique of Milling Thin-wall Nylon Parts

利用光切方法测量微结构的三维微运动

Measuring three-dimensional micro-motions of microstructures using optical sectioning

基于Pro/E工程图肋、轮辐的纵向剖切方法研究

The Research on Vertical Sectioning Method of Rib and Spoke Based on Pro / E Engineering Drawings

基于交流支路电气剖分思想，提出了一种配电网电容器的优化投切方法。

Based on electrical dissection of AC branches , an optimal capacitor switching method of distribution network is proposed .

介绍了集聚纺网圈的织造、热定型、熔切方法，防静电处理和超声波清洗方法。

Introduction is made to the lattice apron regarding hot setting , melt cutting , way of anti-static and ultrasonic cleaning .

然后论述了电容器的运行管理问题，包括电容器的调整、投切方法、电压监测、巡视检查等；

The operation management of capacitor is also described , including capacitor regulation , switching ways , voltage monitoring and patrol inspection etc.

仿真结果表明，当采用该投切方法时，装置的投切过程不会对负载、电网和装置本身带来任何不利影响。

Simulation results show that the course of putting into and withdrawing will not bring any harmful effects to load , electric wire netting and device itself .

针对目前无功补偿装置中采用的单相过零投切方法存在的缺点，本文提出采用模糊控制的方法。

In order to overcome the disadvantage of the single phase passing zero control in the reactive power compensation device , fuzzy control is put forward in this paper .

另外，利用数控仿真比以往的试切方法节省了大量的人力、物力和财力，缩短了产品的开发周期，提高了劳动生产率。

In addition , compared to real trial-machining , NC simulation can save much manpower 、 material and financial resources , cut short the development period of products , raise the labor productivity .

文章就什么情况使用剖视图、如何恰当的选择剖视表达方法提出了一些见解，并对这些剖切方法进行了归纳总结。

This paper puts forward some opinions about the application of cutaway views and the proper use of repression modes of the cutaway views , and makes induction and summarization of the cutting methods .

方法1999年5月至2002年2月应用关节镜下碎切方法治疗膝关节游离体>15cm的患者11例，将游离体钳夹固定后用髓核钳逐步咬切取出。

Methods 11 cases of knee loose body were treated under arthroscopy using biting excision from May 1999 to February 2002 . The loose body were fixed with holding forceps then the loose body were removed using biting excision method .

方法：采用DNA测序及限制性酶切反应方法。

Methods : DNA sequencing and restriction endonuclease reaction was used .

利用OpenGL对岩体三维模型进行切剖面方法研究

Study on Three-dimension Rock Body Cutting Method Using OpenGL

为探寻一种更简单、安全的包皮环切手术方法，对300例患者在局麻下，用CO2激光在直视下行包皮环切术。

To establish a simple and safe mode of circumcision , three hundred patients with redundant prepuce underwent CO 2 laser circumcision under local anesthetic on outpatient department .

方法82例汉族女性雄激素过多症患者及14名健康女性进行ACTH兴奋试验，并应用PCR扩增产生限制性酶切位点方法检测已知的9个21-OHD常见突变位点。

Methods Eighty-two androgen excess cases and 14 healthy women underwent ACTH stimulating test during the follicular phase .

方法应用激光微切的方法从冰冻切片上将单个肝细胞切下，提取总RNA，将RNA逆转录成cDNA，采用巢式逆转录聚合酶链反应（RT-PCR）检测mRNA的表达。

Methods Individual hepatocyte was isolated from cryostat tissue section using laser microdissection technique . To detect the mRNA expressed by single hepatocyte , RNA was extracted , reversely transcripted to cDNA and amplified by nested polymerase chain reaction ( PCR ) .

基于环形光切测量方法的小管径图像测量系统的设计

Design of Image Measurement System for Small Pipe Using LC-CCD Method

跖内侧皮瓣切取方法的改进及应用

Improved method of obtaining medial plantar cutaneous graft and its clinical application

医学图象体数据场的剖切显示方法

The Section Display Method for Medical Image Volume Dataset