分离液

DNA提取：患者骨髓或健康志愿者外周血标本采用淋巴细胞分离液分离单核细胞，传统酚/氯仿/异丙醇法提取DNA。

DNA extraction : Monocytes in bone marrow of patients or whole peripheral blood of healthy volunteers were isolated by Lymphocyte Separation Medium , and then genomic DNA were extracted with the traditional phenol / chloroform method .

材料和方法1.用密度为1.073g／ml的淋巴细胞分离液以及贴壁培养分离骨髓间质干细胞，培养、传代并冻存储备细胞。

Get the human bone marrow mesenchymal stem cells by lymphocyte separation medium ( 1.073g / ml ) and adherent culture . Store cells for further study by passage and freeze cells .

焦化废水泡沫分离液的Fenton催化氧化预处理

Pretreatment of the foaming wastewater from coking wastewater station by Fenton oxidation

电解法处理利福霉素SV钠废渣分离液的研究

Study on treating the separated fluid of rifamycin SV sodium scrap by electrolytic method

三种体系萃取分离液闪测定海水中~（234）Th的比较研究

Comparative study on separation of () ~ ( 234 ) Th in sea water with three extraction systems and determination using liquid scintillation spectrometer

结论：Percoll分离液能明显的提高Leydig细胞纯度；

Conclusions : Highly purified Leydig cells could be obtained with Percoll .

密度梯度离心法：Ficoll分离液组选用的Ficoll分离液密度为1.077；

Density gradient centrifugation : 1.077 Ficoll was used in the Ficoll separating medium group ;

用淋巴细胞分离液分离白细胞，火焰原子吸收法测定Zn、cu、Mn、Mg，方法的回收率在86.9～103.1%间，用于白血病人有实际意义。

Leucocytes in separated liquid of Lymphocytes were Separated by using gradient centrifugation , the contents of Zn Cu Mn and Mg was Determined with atomic absorption spectrometry . The total recovery of the method was 86.9-103.1 % .

结论优化大鼠周龄、分离液的密度、细胞培养方法有助于获得多向分化潜能保持良好的MSCs。

Conclusion The optimization of rats ' age , the density of Percoll , the methods of cell cultivation are conducing to obtaining a pure population of MSCs with good differential activity .

结论：Percoll分离液和全血细胞培养法为有效的骨髓间充质干细胞分离方法。

CONCLUSION : Percoll 's solution and complete blood cells culture are the effective laboratory methods for separation of bone marrow mesenchymal stem cells .

方法预先制备同种异体脱钙骨，取健康成人骨髓，用单核细胞分离液分离MSC，间充质干细胞基础培养基原代和传代培养。

Methods Allogenic decalcified bone matrix was prepared . MSCs were isolated from adult human bone marrow with monocyte-separating medium and then cultured in basic medium of mesenchymal stem cells ( MSC ) .

方法：以凝血酶时间（TT）为指标，用添加法比较不同浓度草酸铵对僵蚕提取液和凝胶分离液抗凝作用的影响。

Methods : With the thrombase time ( TT ) as the index , to compare the influences of different concentration on the action of anticoagulation on the worm 's abstract solution and gel separated solution .

然后用自制的60%和75%的Percoll细胞分离液摸索出3种中性粒细胞的分离方法，即Percoll梯度离心法、Dextran作用下的红细胞自然沉降法和裂解红细胞法。

Then groped three species Segregation methods of PMN by using 60 % and 70 % percoll , namely , percoll gradient Centrifugation , erythrocyte physio-sedimentation was acted by Dextran .

方法参照国内外方法加以改良，应用DHanks液校正淋巴细胞分离液，使其为1053，采用单层一步梯度离心法分离HSC。

Methods Lymphocytic cell separating medium and D Hanks was mixed , and the density of the mixed fluid was adjusted to 1 053.HSC were isolated by monolayer gradient centrifugation method .

方法：1.采用Percoll分离液密度梯度分离法，体外分离及纯化SD大鼠MSCs，经过形态学特征、表面标志物表达及多向分化潜能等多方面鉴定所得细胞。

S-D rats bone marrow MSCs could be separated and purified in vitro by the Percoll density gradient separation , . Morphology characteristics , cell surface markers and the potency of multi-directional differentiation were applied to identify the cells .

方法无菌条件下抽取先天性心脏病婴幼儿胸骨骨髓3~5ml，经密度梯度离心（Percoll分离液，Υ为1.073），接种获得BM-SCs，常规培养。

Methods The bone marrow was extracted from the sternum of infants with congenital heart diseases under sterile conditions . BMSCs were isolated by density gradient centrifugation ( Percoll , Υ 1.073 ) and expanded by cell culture .

密度梯度离心培养实验取剩余6只，以分离液的不同分为Ficoll分离液组、Percoll分离液组，3只/组。

The other 6 rats were used in density gradient centrifugation experiment , which were divided into Ficoll separating medium group and Percoll separating medium group based on different separating medium with 3 rats in each group .

用淋巴细胞分离液获取两组单个核细胞标本并裂解获得其内表达的蛋白质，进行双向凝胶电泳（2-DE），保证每例样本有3次独立重复试验。

Obtain and splitting individual nucleus cells by the lymphocyte separation fluid and cell cracking liquid and acquire all of the protein in cells . Separate these proteins by the method of 2-DE. Guarantee 3 independent repeated experiments for every case . 3 .

方法利用造血干/祖细胞培养、体外扩增、移植动物模型等技术，研究不同比密ficoll分离液分离的脐血造血干/祖细胞的造血活性。

Methods By using a series of techniques , i.e. , stem / progenitor cells culture , expansion in vitro and transplantation in animal model , the hematopoietic activity of fractionated cells in discontinuous Ficoll gradient was evaluated .

方法选取健康献血员30名无菌采集静脉抗凝血，用淋巴细胞分离液分离外周血单核细胞（PBMC），并调成1×106/ml浓度加PHAP10μg/ml。

Methods The peripheral blood monocytes ( PBMC ) were isolated by lymphocyte separation solution form 30 health volunteers , which were diluted to the concentration of 1 × 10 6 / ml with PHA-P ( 10 μ g / ml ) .

脐血经3%明胶+淋巴细胞分离液分离后，脐血单个核细胞的回收率达54.51±14.19%；单个核细胞经贴壁2小时，MACS纯化后，CD34~+细胞富集倍数达36.22倍。

The recovery rate of MNC from UCB was 54.51 ± 14.29 % by 3 % gelatin and Ficoll-Hypaque ( FH ) separation , and the enrichment of CD34 + cells was 36.22 folds by MACS isolation system after MNC incubation for 120 min to remove the adherent cell layer .

淋巴细胞分离液离心后取单核细胞层，D-Hanks液洗涤离心2遍，悬浮于含10%FBS的DMEM培养液中，行原代培养。

The monocyte suspension was aspired after bone marrow was centrifuged by lymphocyte separating medium . And then , the monocyte suspension was washed and centrifuged twice with D-Hanks liquid , suspended in DMEM medium containing 10 % FBS and cultured primarily .

将胃粘膜组织置于不同分离液中，37℃振荡50min，结果显示：胶原蛋白酶、链霉蛋白酶、胰酶、EDTA-Na2单独使用及胰酶与EDTA-Na2联合使用，样本得到的细胞都较少；

Incubating the gastric mucosa in digestion solvent and shaking in 37 ℃ . Treated by collagenase , pronase , trypsin , EDTA-Na_2 separately , and combination of EDTA-Na_2 and trypsin , the cell number released from every sample is low .

离心力分离液固两相流

Separation of Liquid and Solid Particle Two-phase Flow by Centrifugal Force

影响α-淀粉酶分离液澄清度的因素

Effects of Various Factors on Clarity of Separated Alpha Amylase Fermentation Broth

酒精废醪分离液回用研究

Study on Reuse of Centrifugalized Clear Liquid of Alcohol Stillage

应用淋巴细胞分离液纯化大鼠胰岛的实验研究

Experimental study of using lymphocyte to purify islets of rat

使用淋巴细胞分离液和3%的明胶分离胎儿细胞。

Fetal cells were isolated using lymphocyte separation liquid and 3 % gelatin .

快速高分离液质联用技术在农药残留检测中的应用

Study on the Analysis of Pesticide Residues by Rapid Resolution Liquid Chromatography-Mass Spectrometry

蒽油，蒽糊，蒽馏分离液。

Anthracene oil , anthracene paste , anthracene fraction .