细胞毒试验

细胞毒试验表明对肝癌细胞具有杀伤作用，而对正常肝细胞无损伤。

Cytotoxicity test showed the killing activity of expressed product to hepatocarcinoma cells but no damage to normal hepatic cells .

方法：体外细胞毒试验是用噻唑蓝（MTT）法。

Methods : cytotoxicity test in vitro were measured by MTT assay .

方法：用MTT比色方法，细胞毒试验及细胞增殖实验，分析比较不同条件下MTT显色反应的性能特点。

Methods : The colorimetric assay with MTT , and the experiments of cytotoxicity and proliferation of cells .

结果：1.细胞毒试验观察到Hcy促进平滑肌细胞生长与增殖；

Results : 1.Homocysteine had promotive effect on the proliferation of aortic smooth muscle cells ;

应用H2Kb四聚体对特异性CTL检测结果与经典的细胞毒试验一致，同时对胞外IFNγ进行了定量检测。

Specific CTL responses were demonstrated by H 2K b OVA tetramer staining and cytotoxicity assay . Extracelluar IFN γ was also quantitatively analyzed .

结论三次项曲线对MTS法细胞毒试验的细胞数OD关系有很高拟合度且优于线性曲线模型。

CONCLUSION For the relations of cell number-OD in MTS cytotoxicity assay , the fitness of cubic curves are better than the linear model .

本文以NIH标准微量淋巴细胞细胞毒试验对上海地区所见41例白塞（Behcet）病进行了HLA-A·B位点检测。211例正常人作为对照。

HLA-A and B locus were determined by NIH standard microlymphocytotoxicity test on serum from 41 patients with Behcet 's disease and 211 normals as a control .

82株不含有stx2或stx1基因大肠杆菌Vero细胞毒试验均阴性。

Coli without stx 2 and stx 1 were negative in Vero cytotoxin assay .

方法：用51Cr释放法和MTT比色法、NAG比色法，单独或相互比较，检验分析细胞毒试验中效靶细胞相互作用规律，酶在细胞代谢中分布情况，细胞死伤前后酶释放特点。

Methods : 51 Cr release way and the colorimetric assay of MTT and NAG used in the experiment of cell metabolism and cytotoxicity assay .

方法：用细胞毒试验、SDS-PAGE结合薄层扫描研究H.pylori临床分离株空泡毒作用及其与VacA的关系。

Method : The vacuolated effect and its relationship to VacA of H.pylori clinical isolates were investigated by the method of cytotoxic test 、 SDS-PAGE and scan .

本实验用非同位素细胞毒试验方法观察了肿瘤坏死因子（TNFα）对宫颈来源的三个细胞系的体外杀伤作用，并比较重组人干扰素（IFNγ）对TNFα细胞毒性的协同作用。

Experiments were designed to determine the cytotoxicity mediated by tumor necrosis factor ( TNF α) to three cervix derived cell lines in vitro . A synergistic increase in the cytotoxic effects of TNF α and interferon ( IFN γ) was analyzed .

采用补体依赖性细胞毒试验（CDC）和微量序列特异性引物聚合酶链反应（MicroPCRSSP）技术进行HLAI类和II类分型。

Donors and recipients HLA class I typing was performed using complement dependent cytotoxicity ( CDC ) test with special monoclonal tray ( SMT ) and HLA class II gene typing by micro sequence specific primers polymerase chain reaction ( Micro PCR SSP ) .

细胞毒试验：96孔板每孔接种细胞，加不同剂量的药物培养72h，加入MTS培养3h后于490nm波长测定OD，用SPSS110软件对数据作曲线估计。

The regression curves were estimated from the data with the software SPSS 11.0 . Cytotoxicity assay : Cells were seeded into 96 well plate and incubated with drug of different doses for 72 hours . OD was read at 490 nm after incubating with MTS for 3 hour .

改良微量细胞毒试验检测急性白血病外周血T细胞亚群

Examination of T lymphocyte subsets of acute leukemia by improved microcytotoxicity assay

抗血清1∶320倍稀释后与鼠脾淋巴细胞做补体依赖的细胞毒试验，特异性细胞毒性均为32%。

The antibody at a dilution of 1 ∶ 320 could kill about 32 % murine spleen cells in the complement dependent cytotoxicity assays .

马抗卵巢癌抗体体外细胞毒试验与～（131）碘标记抗卵巢癌抗体裸鼠卵巢癌移植瘤聚集性体内分布实验

Study on in-vitro Cytotoxic Assay of Horse Anti-ovarian-cancer Antibody and Distribution of ~( 131 ) I-labeled Anti-ovarian-cancer Antibody in Murine Ovarian Cancer Transplanted Nude Mice

本文用抗胸腺细胞血清细胞毒试验检测了29例HBsAg无症状携带者外周血T细胞数，与正常人（20例）相比显著低下（P<0.001）；

29 cases of HBsAg asymptomatic carriers were assessed by cytotoxicity test using anti-thymus cell serum and by E-rosette test for the number of T cells in the peripheral blood .

酶释放法用于细胞介导的细胞毒试验问题分析

The analysis of the problems of enzyme release way used for cell mediated cytotoxicity assay

为探讨细胞表面HLAⅠ类抗原分子作为细菌受体的可能性，用10属16种21株细菌，8份7个特异性的抗血清，对108份健康志愿者外周血淋巴细胞进行了细胞毒抑制试验。

In order to determine the possibility of HLA class I antigen molecules as bacterial receptor , cytotoxicity inhibition test was carried out with 21 strains of bacteria , belong to 16 species of 10 genera of bacteria , 8 antisera of 7 specificity and 108 healthy volunteer lymphocytes .