传代

结果从SD大鼠胚胎前脑皮质分离的组织经原代和传代培养均可形成细胞克隆，并具有增殖的能力。

Results : Cell clones could from the forebrain cortices of embryonic rat after primary and passage cultivation .

从原始生殖细胞分离昆明小鼠EG细胞及其传代培养

Isolation of Kunming Mice Embryonic Germ Cells Derived From Primordial Germ Cells and Their Passage Culture

氧自由基诱导鸭乙型肝炎病毒DNA整合及其细胞传代研究

Duck hepatitis B virus DNA integration induced by oxidative radicals and its transmission from parent cells to offspring ones

~（60）Co辐照对大鼠肝癌细胞系传代生长的影响

Effects of ~ ( 60 ) Co irradiation on the growth of rat hepatoma cell lines

乙型肝炎病毒重组DNA转化Vero绿猴肾传代细胞分泌乙型肝炎病毒表面抗原

Excretion of HBsAg by Vero cell line transformed by HBV recombinant DNA

形成的NP细胞系大部份不再分泌可检测的白蛋白，少量细胞分泌白蛋白的能力也随着传代而消失。

The NP cells do not retain the ability to express detectable albumin .

通过对新型弱毒株（B株）进行鸭胚传代致弱后，稀释成不同倍数对1日龄雏鸭进行免疫，并于10日龄时用新型鸭肝炎强毒攻毒，观察雏鸭的死亡情况。

It was diluted into different concentration to immunize 1-day-old ducklings and the immunized ducklings were challenged with new serotype duck hepatitis virus in the 10th day .

通过传代扩增培养，获得了MSC纯化细胞。

After subculture , we obtained MSC passage purified cells .

进行MSCs的体外培养和传代扩增。采用贴壁培养法使MSCs得到纯化。本实验通过对T。

MSCs were cultured , proliferated and purified by passage culture . We cultured T.

用胶原酶灌注消化，收集小牛主动脉内皮细胞（EC）进行传代培养。

Arterial endothelial cells were obtained from bovine aorta by mild treatment with collagenase and medium perfusion .

结果表明，在352只G1小鼠中，14只出现异常表型，但均未传代；

The results indicated that in 532 GI mice , there were 14 mice carrying mutant phenotypes , but none of these abnormal phenotypes was inheritable .

小鼠ES细胞条件培养基对绵羊类胚胎干细胞克隆、传代的影响

The Effects of Mouse ES Cell Conditioned Medium on Isolation and Cloning of Sheep Embryonic Stem-like Cells

EHF病毒在传代鸡胚上的实验观察

Experimental observation of epidemic haemorrhagic fever virus on passed chick embryos

通过试验，筛选出对水貂肠道冠状病毒敏感的貂肺传代细胞系（ML），经胰酶处理后，从6份病貂粪样中分离获得2株冠状病毒。

Two strains of coronavirus were isolated from 6 fecal samples of infected minks with mink lung cell line ( ML ) cultures .

传代培养前后内皮细胞差异表达基因消减cDNA文库的构建

Construction of a subtracted cDNA library of differentially expressed genes in freshly isolated adult and cultured new-born bovine aortic endothelial cells

经原代培养及传代培养后获得nestin阳性细胞。

The nestin positive cells were harvested through primary culture and subculture .

肾综合征出血热Vero传代细胞疫苗的研究

Studies on Vero Cell-derived Hemorrhagic Fever with Renal Syndrome Vaccine

目的建立大鼠骨髓间充质干细胞（MesenchymalStemcells，MSCs）体外分离、培养、传代及诱导分化的实验方法；

Objective To establish the experimental methodology of isolating , culturing , proliferating and inducing rat bone marrow mesenchymal stem cells ( MSCs ) in vitro ;

结果：在原代及传代细胞中有Nestin阳性细胞即神经干细胞。

Results : Nestin positive cells were found in the culture and daughter clones .

所以，在本试验条件下和时间范围内，转抗除草剂Bar基因稻谷作为饲料对传代成年小鼠的健康无明显不良影响。

So , in this experimental condition and time range , herbicide resistant Bar gene rice as feed on the passage of adult mouse health without obvious adverse effect .

结论HBV可以在体外感染滋养层细胞，且能在传代细胞中有持续的表达。

Conclusion The HBV can contaminate the JEG3 at vitro , and it was able to had the expression in spread layer cells with generations .

通过传代，在第四代获得了高纯度的BMSCs，细胞形态多呈梭形或成纤维细胞状，胞核大而呈圆形或椭圆形。

BMSCs were fusiform or like fibroblasts . The nucleus of cells was bigger , spherical or elliptical .

多项实验证明，本研究得到早期传代的PGCs源的小鼠、人类ES细胞。

In conclusion , we obtain human , mouse ES-like cell lines derived from PGCs .

传代培养的NSC性能稳定；

The property of NSC was stable after long culture and preservation .

紫外线照射的HSV-2在人胚肺传代细胞上的持续感染

Human embryonic lung cells persistently infected by ultraviolet light - irradiated HSV-2

方法利用SD大鼠间充质干细胞（取自股骨和胫骨）贴壁生长的特性，培养纯化，传代扩增。

Methods MSCs were obtained from the tibia and femur bone marrow of SD rats and cultured by their adherence characteristics to culture flask .

HFRS病毒Z（37）株在绿猴肾传代细胞的病变作用和传代适应

Cytopathic Effect ( CPE ) and Adaption of HFRS Virus Vaccine Strain E_ ( 37 ) in Green Monkey kidney Cell

从南京某肉鸭养殖场用灭菌棉拭共采集肉鸭的泄殖腔样品20份，通过SPF鸡胚尿囊腔传代接种法分离到1株病毒。

A strain of virus was isolated from 20 cloacal of ducks in Nanjing using SPF chicken embryos .

结论：所建立的RTPCR方法可自病毒感染的小鼠脑组织和传代细胞中检测WEE病毒的基因组RNA。

Conclusions : A specific RT_PCR assay was established for detecting the genomic RNA of WEE virus from infected suckling mice and culture cells .

人bcl-xl转基因小鼠外源基因的复制及传代的稳定性观察

Observation on Reproduction and Stable Transmissibility of Foreign Gene in Human Bcl-xl Transgenic Mice