亚细胞蛋白质组

文章全面介绍了亚细胞蛋白质组学的最新研究进展，阐述了亚细胞蛋白质组学面临的挑战，并对亚细胞蛋白质组学的发展方向作了展望。

The recent progress on subcellular proteomics , the challenge and prospect of subcellular proteomics are discussed .

快速发展的亚细胞蛋白质组学

Progress on Subcellular Proteomics

亚细胞蛋白质组学最大的困难在于怎样确认鉴定出来蛋白质的定位，是在提取过程中的污染还是真正在此亚细胞结构中有定位？

However , the challenge of subcellular proteome is how to explain the localization of the identified proteins , which are contaminated or really localized in this structure ?

亚细胞蛋白质组学不仅可以降低蛋白的复杂性，而且可以富集低丰度蛋白，提高低丰度蛋白的检出率，有利于人们深入认识生命本质。

Subcellular proteomics can not only reduce the complexity of the proteins , but also can enrich the low-abundance proteins , and improve the detection rate of low-abundance proteins , which will help better understand the mechanisms of cell physiology .

使用亚细胞蛋白质组分分离提取试剂盒，提取过程提供4个蛋白组分以降低蛋白复杂性。

Each time the pellet is mixed in the rotatory shaker at 4 ℃ and centrifuged in a refrigerated centrifuge . Cells were fractionated using sub cellular proteome extraction kit . The extraction procedure provided four fractions with decreased proteome complexity .

目的：探索胶上蛋白原位酶切、肽质量指纹图（PMF）分析以及数据库检索方法的优化条件，建立适合于亚细胞比较蛋白质组学研究的质谱鉴定策略。

Objective : To explore the optimal conditions associated with proteolytic digestion in 2-DE gel , PMF analysis and database inquiry , and to construct a mass identification strategy in research for mitochondrial comparative proteomics .

本研究运用亚细胞比较蛋白质组学的研究策略和技术方法，以奶牛乳腺组织为研究模型。

Sub-cellular comparative proteomic research strategies and techniques were applied in this study .

植物亚细胞结构蛋白质组学研究进展

Progress in sub-cellular structure of plant proteomics research

肝癌亚细胞结构的蛋白质组分比较分析

Comparative proteome analysis of hepatoma cells at subcellular level

传统的亚细胞分离技术结合质谱鉴定技术为亚细胞蛋白质组学的研究提供了技术平台。

The classical subcellular fractionation methods coupled with mass spectrometry identification technique have created opportunities for the study of subcellular fractions .