电转化

同时，发现电击后的细胞必需在高渗培养基中才能存活，电击后2～3h的复苏表达期和用亚抑制抗生素浓度选择转化子，这些对电转化成功以及提高转化效率都是十分关键的。

A post pulse recovery time of 2 ~ 3h and the use of sub inhibitory concentrations of antibiotics in the selective plates were very critical for electrotransformation .

提高黄色短杆菌C-11电转化效率的方法

Methods of increasing efficiency of electrotransformation of B.flavum C-11

通过电转化将表达质粒转入T7重组菌获得4种脂肪酶工程菌。

The above vectors were respectively electroporated into T7 recombination strains and obtained four lipase expression strains .

电转化构建的噬菌体抗体库的库容为1.3×106，Fab基因的重组率为60%。

A human phage antibody library consisting of 1.3 × 106 clones was constructed by electrotransformation and 60 percent of the clones contained Fab genes .

方法：构建真核表达载体PEF/GPI-Met-RANTES，利用电转化法转染CHO-DHFR-细胞，氨甲喋呤（MTX）筛选抗性克隆。

Methods : The eukaryotic expression vector PEF / GPI-Met-RANTES were constructed and transfected into CHO cells by electroporation . The transfectants were selected with methotrexate ( MTX ) .

结果质粒电泳图谱显示，转化子DH5α所含质粒与肺炎克雷伯菌所含质粒相同，电转化取得成功；

Results Electropherogram showed that the plasmids in DH5 α were the same as those in Klebsiella pneumonia and electroporation was successful ;

将重组质粒电转化毕赤酵母GS115感受态细胞，甲醇诱导蛋白质表达。

The recombinants were induced by methanol for the expression of recombinant protein .

结果：电转化电压在2.1～2.5kV之间可获得高转化效率；

Results : A high electro transformation efficiency was obtained when the voltage was set between 2.1 to 2.5 KV ;

方法抗原基因vp4经PCR扩增后直接克隆到pUCT载体，双酶切目的片断和植物表达载体，以T4连接酶将目的片断与载体相连接，电转化方式将重组质粒转入农杆菌。

Methods PCR product of vp4 gene was directly cloned into pUC T vector , and the vp4 DNA fragments were cut down by restriction enzymes and inserted into the plant expression vector . The recombinant plasmids were transformed into Agrobacterium tumefaciens by electroporation .

电转化方法将外源性质粒导入干酪乳杆菌的研究

Study on exogeneous plasmids transformed into Lactobacillus casei Zhang by electroporation

铜绿假单胞菌最佳电转化条件的研究

The Study of Optimal Conditions of Electroporation in Pseudomonas aeruginosa

高效电转化技术在噬菌体抗体库构建中的应用

Application of Highly Effective Electricity Transformation Technology in Bacteriophage Immune Body Storehouse Construction

高渗透压对细菌电转化率的影响

Effect of High Osmolarity on Electrotransformation Efficiency of Bacteria

选择最佳电转化条件能显著提高转化效率。

The transformation efficiency can be obviously raised by using the optimal electroporational parameters .

电转化法及其在苏云金杆菌中的应用

Electroporation and its application to Bacillus thuringiensis

传统的粘细菌遗传分析方法，比如转导、电转化和接合的效率很低，非常耗时。

Conventional genetic analyses of myxobacteria were inefficient and time consuming such as transduction , electro transformation and conjugation .

单晶硅太阳能电池因其电转化率高，制造工艺成熟，可靠性好而首先被用于航天领域。

Because of its high conversion efficiency , mature producing techniques and good reliability , solar cell was firstly used in space field .

同时，在建立突变体库的过程中，对大肠杆菌电转化条件进行了摸索，确定了电转化的最佳方式。

At the same time , when mutant library was established , we explored different transformation methods to determine the best conditions for electroporation .

同时利用根癌农杆菌介导的转化、醋酸锂法和电转化的转化方法，对菌株34-9转化条件进行了摸索，并对转化子进行了初步鉴定。

It also conducted a preliminary exploration of transformation system of strain 34-9 by using Agrobacterium-mediated transformation , lithium acetate transformation and electroporation transformation .

由于乳酸菌为革兰氏阳性菌，具有很厚且致密的刚性细胞壁，受体菌的转化通常采用电转化方法进行转化。

Due to thick compact cell wall of gram-positive bacterium , to which category LAB belongs to , the transformation of LAB is always using electroporation .

逆变电源是一种将直流电转化成输出电压和频率稳定交流电的DC/AC转换器，其核心技术为现代电力电子学。

Inverter is a DC / AC converter of outputting alternating current voltage and stability frequency . The technology of inverter is the modern power electronics .

来自受体菌自身的同源质粒，因克服了宿主的限制一修饰性，可以极显著地提高电转化效率。

A significant increase of transformation efficiency was revcaled with the homologous plasmid isolated from recipient itself since the effects of host restriction and modification werc avoided .

目前使用的基因突变方法，如自然转化，接合转移以及自杀质粒电转化的转化效率和发生同源重组双交换的效率比较低下，所以一套高效的突变系统的建立是非常必要的。

The current methods of mutation including natural transformation , conjugation and electroporation using suicide plasmid are defective in the efficiency of transforming and double exchange of homologous recombination . So it is necessary to establish a set of effective mutation system .

LED技术是一种利用半导体芯片研发出来的可以将电能转化为光能的全照明技术。

LED , a novel technology , can change electric energy to light energy by making use of semiconductor chip .

发光二极管（LightEmittingdiode，LED）是一种能够将电能转化为可见光的固态的半导体器件。

ABSTRACT : Light Emitting Diode , LED , is a device capable of converting electrical energy into visible light solid-state semiconductor devices .

具体而言，利用水电解将电能转化为储存于H2和02中的化学能；利用燃料电池将H2和02复合成H20释放出电能。

Specifically , the electricity can be converted to chemical energy stored in H2 and O2 through water electrolysis , and these two molecules can be re-combined into H2O to output electricity through fuel cells .

把电能转化成声音能量或者把声音能量转成电能的传感器。

A transducer that converts electrical to acoustic energy or vice versa .

把电能转化为机械能的发动机。

A motor that converts electricity to mechanical work .

工厂正在把电能转化为光。

The plant is transforming electrical energy into light .

步进电机是一种将电脉冲转化为角位移的装置。

AT89C2051 Abstract Stepping Motor is the equipment which change pulse into electricity angle .