电泳图
- 名electropherogram;electrophoretogram;electrophoresis pattern;mobility spectrum
![电泳图](../cache/1190143.jpg)
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本文通过对模拟数据和实验数据的分析,研究了电泳图的n-阶导数在手性药物定量方面的应用。
In this work , the use of n-th derivative of the electropherogram was studied and examined on model and experimental data .
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以阿魏酸、腺苷的提取效率及电泳图中峰数目的多少为指标,对样品制备方法进行评估。
Extraction methods were evaluated for the extraction efficiency of adenosine and ferulic acid , as well as for the number of peaks in the electropherogram .
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运用凝胶成像系统中的定量软件Quantityone对各电泳图上的各条带进行定量分析,将图形结果转换为数据结果。
Use a quantitative gel imaging software Quantity One to quantify each band in the electrophoresis map and then convert the graphical results into digital results . 4 .
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重组病毒感染BHK21细胞后,可见由于细胞凋亡而致的染色体DNA断裂梯子电泳图。
When infected with the recombinant viruses , the chromosome DNA ladder caused by the apoptosis of the BHK cell was observed .
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结果表明DNA与药物作用后,Taq酶切电泳图发生明显改变,说明药物与DNA的结合位点可能在两种酶的酶切位点T/CGA上。
The results show that after of drug action , Taq estriction enzyme eLectrophoresis changed significantly . Show that Drugs and DNA binding sites o may be in two enzymes restriction site T / CGA .
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对蔗糖酶的SDS凝胶电泳图进行扫描分析的结果表明,经SFE处理后的蔗糖酶含量比对照高两倍左右。
The result of scanning on SDS PAGE gels showed that the invertase content after treatment by SFE was twice as high as that of the control .
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发酵液经(NH4)2SO4沉淀、DEAE-纤维素层析和凝胶过滤分离出二种几丁质酶组分,在聚丙烯酰胺凝胶电泳图上显示出两条均一的带,并且每条带都具有几丁质酶活力。
Two extracellular chitinases were purified to homogeneous by ( NH4 ) 2SO4 precipitation , DEAE-cellulose chromatography and gel filtration .
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裸小鼠诸器官及血清蛋白的双向电泳图
Mini Two-dimensional Electrophoregrams of Serum and Soluble Protein in Organs of Nude Mouse
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用酪蛋白平板法和电泳图相比较法的活性筛选方法从基因文库中筛选到一株阳性克隆(命名为pHH1)。
One positive clone was obtained from the libraries with casein flat and electrophoresis comparison method .
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单向电泳图中存在20kD左右、胚分化完成期以后特有的蛋白质。
In the SDS-PAGE patterns proteins with molecular weight of approximately 20 kD were present when embryo differentiation was complete and later ( Fig. 1 ) .
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经统计学分析,辐照与未辐照鸡肉细胞彗星电泳图尾长与尾相存在显著性差异,并存在一定的剂量反应关系。
The statistics analysis shows that the tail length and the tail moment shows big differences by different irradiation levels and increased with the gaining doses of irradiation .
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以Northern杂交、形态观察、流式细胞光度法(FCM)分析及DNA电泳梯形图,分别证明转染细胞中该基因表达及凋亡诱导。
The gene expression and apoptosis of transfected HL 60 cells were detected by Northern blot , morphological assay , FCM and ladder map of DNA electrophoresis , respectively .
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结果:龙眼肉及其混淆品蛋白多肽高效毛细管电泳谱图有明显的差异。
Results : Arillus Longan could be distinguished from it 's confused variety by the proteins electrophorograms .
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富集低丰度蛋白的人类精子全蛋白二维电泳整合图的建立
Establishment of the 2-D Synthetic Map of Total Protein of Normal Human Spermatozoa Enriched with Low Abundance Protein
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从毛细管电泳谱图能直接观察胰岛素去折叠过程中发生的变化,获得蛋白质去折叠信息。
The structural change during the process of bovine insulin unfolding could be observed directly from the electropherogram and information of protein unfolding could be obtained simultaneously .
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优化了小麦幼苗叶片蛋白的提取、分离和染色技术,获得了分离清晰、蛋白点多的电泳胶图。
Optimized the 2D technology including protein extraction , separation and staining techniques in leaves of wheat seedling , obtained a map which has clear separation and numbers of proteins .
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超速离心法:电泳分析图可见,处理后的样本电泳条带亮度高,但有拖尾现象,且通过电镜分析可知,背景纯度不高,且含有大量的杂质。
Ultracentrifugation : Electrophoresis analysis diagram shows , the sample is high brightness of band , but tailing phenomenon , and electron microscope analysis , the background is not good , containing a lot of contamination .
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以激光粒度仪粒径分布测量数据为基准,结合β分子筛电泳谱图,建立了β分子筛粒径迁移时间校正方程及含量校正方程。
The calibration equation between migration time and particle size was established to obtain the data of particle diameter by relating the β zeolite diameters determined by laser particle sizer with the migration time from electropherogram of β zeolite through the cumulative percentage of particle distribution .
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用小电泳进行DNA指纹图分析探讨
The study of DNA fingerprinting with small agarose gel electrophoresis
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采用亲和层析的方法对细胞破碎液进行纯化,通过SDS-PAGE电泳分析,在电泳图上获得一条纯化后的单一条带。
Affinity chromatography using the method of cell disruption liquid was purified by SDS-PAGE electrophoresis analysis , gel electrophoresis obtained after purification of a single band .
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叶黄素作用48h后单细胞电泳显示,叶黄素组细胞呈现彗星状电泳图,说明叶黄素可造成MCF-7DNA损伤。
After treated with lutein 48h , the DNA damage of MCF-7 was detected by single cell gel electrophoresis assay , lutein cells show comet-like electrophoretogram .
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通过非常简单的样品前处理过程和合适的电泳条件,在5分钟之内即可得到猪肉样品中的氟喹诺酮的电泳图。
After the tissue sample procedure , we can get the electropherogram of fluoroquinolones within 5 minutes in proper electrophoresis conditions .
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PCR-RFLP(?)电泳结果显示由于突变的存在,使突变基因产生了新的限制性内切酶酶切位点,突变组基因被切为大小不同的两个片段,凝胶电泳图上出现新的条带。
The results of PCR-RFLP electrophoresis showed that the mutant gene produces a new restriction enzyme cutting sites because of the presence of mutations , so mutation gene was cut into two fragments of different size , new bands appeared on gel electrophoresis map .