引物设计

用于聚合酶链式反应（PCR）引物设计的计算机程序

Computer Programs for Polymerase Chain Reaction ( PCR ) Primer Design

本文报道了两个用于PCR引物设计的计算机程序PCRDESN和PCRDESNA。

We have developed two computer programs for PCR primer design .

进化DNA数据的引物设计序列统计软件。

SeqState-primer design and sequence statistics for phylogenetic DNA data sets .

传染性法氏囊病病毒的PCR引物设计与Internet检索分析

Design of Infectious Bursal Disease Virus PCR Primer and Internet Retrieval

本文介绍了PCR引物设计软件的一般设计原理并设计了相关软件。

This paper introduces the design of software of PCR primer design .

四种基因甲基化特异性PCR引物设计探讨

Study of the primer design on methylation specific PCR

利用软件PrimerPremier5.0进行PCR引物设计的研究

The Research of Applying Primer Premier 5.0 to Design PCR Primer

基因芯片的PCR引物设计

PCR primers design for gene chip

随着PCR技术的发展，引物设计成为一个重要的问题。

With the development of the PCR technology , primer design has become an important issue .

引物设计是PCR技术中最重要的一环。

PCR-primer design is the most important step in polymerase chain reaction ( PCR ) technique .

参考鳗鲡等鱼类线粒体DNA序列进行了中国花鲈线粒体DNA细胞色素b基因片断的引物设计、PCR扩增及其序列测定。

The primers of mitochondrial DNA cytochrome b gene of Chinese sea bass were designed by referencing the sequences of Anguilla species .

石斛属RAPD分析及鉴定铁皮石斛的特异性引物设计

Cluster Analysis of Dendrobium by RAPD and Design of Specific Primer for Dendrobium candidum

这里，给出了亚硫酸氢盐转换，引物设计和PCR条件优化的一个详细的实验方案。

Here , a detailed protocol for bisulphite conversion , primer design , and optimization of PCR conditions is given .

牛CD18编码全基因特异引物设计与PCR扩增

Design of Primer Specific for The whole CD18 of Bovine and PCR Amplification

大肠杆菌asd基因PCR引物设计及其长模板PCR扩增

The Primers Design of the Asd Gene of Escherichia coli and its Amplification with Long Template PCR System

扩增的片段经过拼接后获得了一个长片段DNA序列。此片段经测序后，作为RT-PCR引物设计的依据。

A sequence assembly was produced from these amplified fragments , sequenced , and used in the design of primers for RT-PCR .

对2267条EST进行引物设计，共获得237对引物。

A total of 237 primer pairs were successfully designed based on the 2267 SSR-ESTs .

该系统可进行核酸及蛋白质序列统计、性质分析、PCR引物设计、联配及同源性分析等。

This system is convenient for nucleic acid and protein sequence statistic , property analysis , PCR primer design , alignment and homogeneity analysis .

PMT整合了目前几乎所有的引物设计原则，并且用到最新的最近邻居模型（Nearestneighbormodel）。

We have integrated almost all the now-existing primer designing criteria into PMT suite , and have used the latest Nearest-neighbor model .

文章就LAMP法的反应原理、引物设计及其在水产动物病原快速检测中的应用等做简要的综述。

This review briefly summarizes the principle of LAMP , design of primers , and the application for the rapid detection of aquatic animal pathogens .

探索了克隆复杂DNA序列时引物设计的特别规则、反应体系的改进、DNA聚合酶的选用等措施。

The conditions for amplifying high GC content repetitive sequences such as the special rules of primer design , the improvement of reaction system , the selection of DNA polymerase were discussed .

对mRNA差异显示技术中引物设计、应用非放射性标记、优化PCR参数、假阳性鉴定的方法等方面进行了概述。

Primer design , application of non-radioactive marker , optimization of parameter of PCR , some methods of false-positive identification of mRNA differentially display technology were summarized in this paper .

实验结果表明：使用己知DNA序列作引物设计板模，在同源序列碱基变异率不大于6.1%的条件下，通过设计和使用多对引物可保证能扩增出不同物种的同源单拷贝基因片段。

The results proved when base-different rate is below 6.1 % , using a few primers designed from the sequence of one species to amplify the homologous sequence of different species can ensure the success .

引物设计：HBV基因组不同区段分别设计2套巢式引物，比较扩增效率，选择扩增效率高的引物。

Primers design : to design and compare the effectivity of different set of nested primers which were located at s gene , X gene and c gene respectively .

使用PrimerPremier5.0软件对微卫星序列进行引物设计，最后设计得到266对微卫星引物可用于海带分子遗传学研究。

Using Primer Premier 5.0 software to design the microsatellite primers and finally obtained 266 microsatellite primer-pairs for the molecular genetics study of Laminaria .

多重PCR检测猪瘟病毒、猪细小病毒和猪伪狂犬病病毒的研究&猪瘟病毒、猪细小病毒和猪伪狂犬病病毒多重PCR引物设计

Studies on the Detection of Classical swine fever , Porcine parvovirus infection and Porcine pseudorabies by Multiplex Polymerase Chain Reaction I Primer & Design of Multiple PCR Detection of CSFV , PPV 、 PRV

参考果蝇与蚤状■线粒体DNA12SRRNA基因片段序列进行了日本绒螯蟹太平洋群体和日本海群体的相同片段的引物设计、PCR扩增及序列测定。

The primers of Pacific and Japan-sea populations of Japanese mitten crab were designed by using the sequences of mtDNA 12S rRNA region of Drosophila yakuba and Daphnia pulex .

本项研究经PCR引物设计后，以大熊猫和朱鹮的基因组DNA为模板，克隆了大熊猫和朱鹮的GDNF成熟蛋白基因。

Based on the GDNF gene sequence of human and chick , we cloned partial DNA sequences encoding mature protein of GDNF from genomic DNA of giant panda and crested ibis through PCR amplification .

小麦Glu-D3和Glu-B3位点LMW-GS基因特异引物设计与PCR扩增

Development of Primers Specific for LMW-GS Genes at Glu-D3 and Glu-B 3 Loci and PCR Amplification

阐述和分析了CHD基因在扩增目标及引物设计、取样范围及辅助技术和所涉足的科研领域等方面的发展及现状。

This paper expatiated and analyzed the progress and status in amplificative target and design of primer 、 range of sampling and assistant technique and the relative research fields of CHD gene .