基因重组

cre/loxP系统介导的基因重组研究进展

Recent Progress in Research on Gene Recombination Mediated bycre / lox P System

结论重组pcr技术是十分有效、可靠的体外基因重组方法。

ConclusionThe result indicates that recombinant PCR is a very effective and reliable method for gene recombination in vitro .

基因重组构建AFPmRNA的realtimeRT-PCR标准定量模板

Construction of standard quantitative templates of AFP mRNA for real time RT-PCR by gene recombinant

人IL-10基因重组复制缺陷型腺病毒DNA的构建

Construction of replication - deficient human IL-10 recombinant adenovirus

旋毛虫基因重组抗原快速ELISA诊断试剂盒的研究

Study on an ELISA Kit with Recombinant Antigen for Rapid Diagnosis of Swine Trichinellosis

磁性免疫微球的合成及其在基因重组IL-2纯化中的应用

Synthesis of immunomagnetic microsphere and its separation for recombinant human IL-2

目的构建HCVC基因重组腺病毒，以期用于HCVC蛋白的功能研究。

Objective To construct the recombinant adenovirus containing HCV C gene for functional study on HCV C protein .

猪Ⅱ型圆环病毒Cap基因重组腺病毒表达载体的构建

Construction and identification of recombinant adenovirus containing the cap gene of type ⅱ porcine circovirus

方法：（1）利用基因重组（geneticrecombination）制备人组织激肽释放酶。

In the present study : ( 1 ) We used the genetic recombination method to produce kallikrein .

基因重组αA-干扰素体外诱导人外周血NK细胞的活性

Gene recombinant α A - IFN induced natural killer cell activity in vitro

人粒细胞集落刺激因子基因重组及在E.coli中的表达和鉴定

Recombinant Expression and Identification of Human Granulocyte Colony-Stimulating Factor cDNA in E.coli

目的优化基因重组人突变型471肿瘤坏死因子α（TNFα）工程菌的发酵和表达条件。

Objective To optimize the conditions for the fermentation of human mutant 471 TNF α engineering bacterial strain .

结果与结论：基因重组α半乳糖苷酶的N端序列与理论序列完全一致；

Results and conclusion : The results showed that the N-terminal amino acids sequence of recombinant α - galactosidase were identical with theoretical sequence completely ;

含突变型p53和LMP1双基因重组质粒的构建与鉴定

Construction and Identification of Recombinant Plasmid Containing Mutant p53 and LMP1 Genes

转化是细菌基因重组最常见的方式之一，转化的结果可使细菌的染色体整合上外源DNA，从而使其某些生物学特征发生变化，并能代代遗传。

Transformation is one of the most common manners invovled in gene recombination by integrating exogenous DNA to bacterial chromosome , thus causing changes in some biological features .

基因重组BCG菌苗的研究

Study on the Gene Reconstruction of BCG

恶性疟原虫CSP基因重组蛋白及DNA免疫诱导小鼠体液免疫应答比较3.岭南黄鸡肌肉生长抑制素基因成熟肽的克隆及其真核表达载体的构建

Humoral immune responses in mice to the recombinant expressed protein and the nucleic acid vaccine of circumsporozoite protein gene of Plasmodium falciparum

方法应用扩增小鼠Doc1R基因组序列时使用的引物，以基因重组扩增后得到的菌落为模板，直接进行PCR扩增。

Method The recombinant colonies were transfered into the PCR reaction mixture . The PCR primers were used for constructing mouse Doc 1R genomic sequence .

小鼠Endostatin基因重组克隆的构建及鉴定

Construction of Mouse Endostatin Gene Recombinant Clone and its Identification

目的评价基因重组抗原ELISA法在梅毒螺旋体抗体检测中的意义。

Objective To evaluate an enzyme-linked immunosorbent assay ( ELISA ) technique based on recombinant antigens for detection of antibodies against Treponema pallidum .

分泌表达人IL-12基因重组卡介苗菌株的构建和筛选

Construction and Screen of Recombinant BCG Strain Expressing and Secreting Human Interleukin 12 Protein

变形链球菌表面蛋白PAc功能区基因重组质粒的亚克隆构建

Subcloning Construction of a Recombinant with Functional Regions of PAc Protein Encoding Gene of S. mutans

不同因素HCV结构区基因重组体诱发小鼠产生抗体的影响

The Effect of Different Administrations of pCD-HCV 1 on Antibody Responses in Mice Immunized by Gene Recombinant of HCV Structure Region

利用基因重组原理，构建可表达CNTF的腺病毒。

The CNTF recombinant Adenovirus was constructed using the Adenovirus system .

方法：通过基因重组、原核表达并纯化阳HPVE1蛋白片段；

Methods : A portion of E1 protein of HPV was purified by DNA recombinant and prokaryotic expression .

戊型肝炎病毒（HEV）合成肽及基因重组抗原免疫反应性研究

Immunoreactivity studies of the synthetic peptides and recombinant antigens with sera from patients infected with hepatitis E virus

我国研制的乙型肝炎基因重组（CHO）疫苗对乙型肝炎病毒母婴传播阻断效果观察

Study on Protective Efficacy of CHO Expressed Recombinant HBsAg Vaccine in Blocking Mother-Infant Transmission of Hepatitis B Virus

目的为建立环境雌激素基因重组酵母测评系统，构建雌激素效应元件（estrogenresponseelements，ERE）调控的酵母报告载体。

Objective To establish the recombinant yeast estrogen system ( RYES ), a reporter vector controlled by estro-gen response elements ( ERE ) in yeast was constructed .

c-jun反义基因重组体转染对缺氧复合烧伤血清刺激下大鼠心肌细胞的保护作用

Protective effects of c-jun anti-sense gene recombinant transfection on rat cardiomyocytes inflicted by hypoxia and burn serum

表达马立克氏病病毒gl基因重组鸡痘病毒的构建及其免疫保护作用

Construction of Recombinant Fowlpox Virus Expression Glycoprotein I of Marek 's Disease Virus and Immune Protection Effect