噬菌体展示文库

微小隐孢子虫T7噬菌体展示文库的构建

Construction of T7 phage display library from Cryptosporidium parvum

结论成功构建了微小隐孢子虫T7噬菌体展示文库。

These results suggests that the T7 phage display library from C. parvum is constructed successfully .

重组Ig结合分子噬菌体展示文库的构建及选择研究

Construction and Selection of Phage Displayed Library of Recombinant Ig-binding Molecules

人工进化丙型肝炎病毒（HCV）C和E1区噬菌体展示文库的构建及初步筛选

The Construction and Primary Screening of Phage Display Library of Hepatitis C Virus C and E1 Evolved with Artificial Pattern

对虾血细胞T7噬菌体展示文库构建及功能基因淘选

Construction of T_7 Phage Display Library from Chinese Shrimp 's hemocytes and Panning of Functional Genes from the Library

通过T7噬菌体展示文库筛选免疫相关基因在安氏隐孢子虫上的应用未见报道。

There is no reported that T7 phage display library screening is used for screening immune-related genes in C. andersoni .

以细菌刺激后提取的对虾血细胞mRNA为材料，构建中国对虾血细胞的T7噬菌体展示文库，并且进行文库扩增，扩增后文库的滴度达到10~（11）pfu／ml。

T_7 Phage Display Library was constructed from challenged shrimp 's hemocyte mRNA , and then the library was amplified to reach the titer of 10 ~ ( 11 ) pfu / ml.

目的：构建HIV-1GagPR蛋白P2/NC切割位点序列随机化的噬菌体展示文库，研究HIV-1蛋白酶（proteasePR）耐药性突变对其敏感切割序列的影响。

Objective : To construct the phage display library of randomized P2 / NC protease cleavage sequences in HIV-1 Gag protein , so as to provide evidences for studying the effect of drug resistance-associated mutations on susceptible cleavage sequences of HIV-1 PR.

目的通过构建汉滩病毒M片段的噬菌体展示文库，筛选汉滩病毒包膜蛋白的抗原表位。

Objective To study the epitope of Hantaan virus M fragment by phage display .

结论所构建的噬菌体展示文库的库容和多样性符合筛选的要求。

Conclusion The capacity and diversity of the constructed library are enough for screening .

噬菌体展示文库的筛选技术

The Selection Techniques of Phage Display Libraries

结果制备了库容为8×107pfu/ml的噬菌体展示文库。

Results A recombinant phage display library with total of 8 × 10 7 pfu / ml was established .

目的：构建由免疫球蛋白结合分子单结构域随机组合的噬菌体展示文库，为应用各种类型免疫球蛋白对该文库进行体外分子进化筛选研究打下基础。

Objective : To construct a phage-displayed random combinatorial library of single domains of immunoglobulin-binding molecules , providing reference for in vitro molecular evolution study of the library with different immunoglobulins .

类风湿关节炎滑膜组织T7噬菌体展示cDNA文库的构建

Construction of T7 phage display cDNA library from synovium of rheumatoid arthritis patients

人源性抗汉坦病毒抗体VH基因噬菌体表面展示文库的构建与筛选

Construction and screening of phage display library of VH gene in human anti-Hantaviruses antibody

目的构建人抗汉坦病毒（HV）抗体重链可变区（VH）基因噬菌体表面展示文库，筛选人源性抗HV单克隆抗体（mAb）。

Aim To construct phage display library of VH gene in anti-Hantaviruses ( HV ) antibody and screen humanized anti-HV mAb .

本实验室用东方田鼠血清、肝脏以及肺脏等组织筛选日本血吸虫噬菌体cDNA展示文库，获得多个有效阳性克隆。

Several positive clones were obtained in the previous work through screening a phage display cDNA library of S. japonicum with fresh sera or extracts from the liver and lungs of M. fortis .

结论成功地构建了人抗HV抗体VH基因噬菌体表面展示文库，并筛选到有HV结合活性的重组噬菌体克隆，为制备人源性抗HVmAb奠定了基础。

Conclusion Phage display library of humanized anti-HV antibody VH gene was constructed successfully , and recombinant phage clones with HV-binding activity were screened , thus laying the foundation for preparation of humanized anti-HV mAb .

人源性胃癌抗体IgG1Fab噬菌体表面展示文库的构建

Construction of phage display library of IgG1 Fab antibodies from lymph nodes of gastric cancer patients

从噬菌体展示抗体文库筛选对虾白斑综合征病毒的单链抗体

Generation and Characterization of a Single-chain Antibody Fragment against White Spot Syndrome Virus of Shrimp from Phage Display Antibody Library

本研究分为2部分：1.构建猪IFN-γ的禽源噬菌体展示抗体文库。

The research is divided into two parts : 1 . Construction of phage display antibody library against porcine IFN - λ .

目的构建抗P-选择素的全套单链抗体噬菌体表面展示文库，从中筛选与P-选择素高亲合力的单链抗体，为其临床应用奠定基础。

Objective Anti-activity platelet phage display library was constructed from mouse immunized with P-selectin , which has been used in clinical usage .

基于噬菌体表面展示随机肽文库的高通量筛选是目前寻找靶蛋白结合短肽的优选策略之一。

The fusion protein was used as target protein to screen phage display random peptide libraries for PreS-interacting peptides .