双向电泳

在此基础上，采用固相pH梯度凝胶双向电泳技术，进行龙眼体胚发生早期胚性培养物蛋白质分离技术研究。

Immobilized pH gradients ( IPG ) two-dimensional electrophoresis was developed to investigate the separation of proteins from early embryogenic cultures of longan .

胃癌细胞SGC-7901双向电泳系统的建立及优化

Establishment and Optimization of Two-dimensional Electrophoresis for Proteomics of Human Gastric Cancer SGC-7901 Cells

IEF/SDSpage双向电泳技术的建立

The building of Capillary BEF / SDS PAGE Electrophoresis Technology

SD大鼠视网膜蛋白质组双向电泳图谱建立和初步分析

Establishment and preliminary analysis of the two-dimensional gel electrophoresis map of neural retinal proteome in rats

利用流式细胞仪分析DNA含量，结果表明此转基因植株为3倍体植株。4.通过双向电泳技术，共得到了368个差异蛋白。

Flow cytometry results suggested that the transgenic plant was triploid . 4 . Using the Two-Dimensional Gel Electrophoresis , we found 368 protein variances .

分别提取了全菌体蛋白、膜蛋白、外膜蛋白以及胞外分泌蛋白，进行了不同pH梯度的双向电泳。

Whole cell proteins , membrane proteins , outer membrane proteins and extracellular proteins were extracted and performed 2-DE of different pH gradient .

不同浓度和梯度的SDS-PAGE胶对双向电泳中蛋白分离的影响

Effect of Concentrations and Gradient of SDS-PAGE Gel on Proteins Separation in Two-Dimensional Gel Electrophoresis

双向电泳分析结晶型NiS诱发人支气管上皮细胞恶变后的蛋白表达差异

Differential Protein Expression in Crystalline Nickel Sulfide-induced Malignant Transformed Cells by Two Dimensional Electrophoresis

方法用SDS-PAGE和双向电泳方法，对日本血吸虫两性成虫全虫可溶性抗原进行分析。

Methods The soluble antigens of the parasites were analysed by means of SDS PAGE and two dimensional gel electrophoresis .

为鉴定莲种子中的高温响应蛋白，从未处理的对照种子和90°C处理24h的种子的胚中提取蛋白经双向电泳分离用于比较蛋白质组学研究。

To identify proteins responsive to high temperature in sacred lotus seeds , both treated and untreated protein samples were resolved by 2D gel electrophoresis for comparative proteomic analysis .

方法：采用双向电泳免疫印迹检测法分析了83例CHD患者血清HDL亚类组成及相对百分含量。

Methods : The contents of serum HDL subclasses in83 CHD subjects were determined by two dimensional gel electrophoresis associated with immunodetection method .

对第一向为载体两性电解质pH梯度等电聚焦双向电泳（ISO-DALT）中若干影响分离效果的实验条件进行了研究.结果发现，琼脂糖和尿素的纯度明显影响分离效果；

The effect of several experimental conditions on the result of ISO-DALT was investigated in this paper .

肺腺癌SPC-A1细胞双向电泳图谱的优化

Optimization of 2-D electrophoresis map of pulmonary adenocarcinoma cell strain SPC-A1

本文应用双向电泳技术，对He-Ne激光辐照增强UV-B辐射后小麦叶片蛋白的损伤修复作用进行了研究。

In this paper , He-Ne laser irradiation enhanced the role of protein in leaves of wheat after UV-B Radiation damage Repair two-dimensional electrophoresis .

通过分离肿瘤坏死因子TNFα诱导的小鼠黑色素瘤B16细胞的细胞核并进行二维电泳分析，获得了分辨率和重复性较好的双向电泳图谱。

The nuclear proteins were extracted from the purified nuclei of TNF - α - induced apoptotic B16 cells , and then analyzed by 2-dimensional electrophoresis .

通过双向电泳，我们发现rds鼠视网膜蛋白质表达与正常鼠相比有质和量的差异。

The expressed proteins in the rds retina are different in quality and quantity from that in the normal C3B mice .

结果应用热SDS法处理血清总蛋白质进行双向电泳，可获得分辨率高、重复性好的人血清双向电泳图谱。

Results The total proteins treated with hot SDS method were used to perform 2-DE. 2-DE patterns with high resolution and reproducibility were obtained for human serum samples .

对嫁接植株和自根植株在NaCl胁迫下叶片可溶性蛋白质进行双向电泳（2-DE）对比研究。

Comparison of the differences in protein components by two-dimensional electrophoresis analysis in leaves was made between own-root and grafted cucumber plants under NaCl stress .

恶性胶质瘤细胞SHG-44诱导分化后的差异蛋白质组双向电泳分析

Analysis of differential proteome of malignant glioma SHG-44 cells after induced differentiation with two-dimensional gel electrophoresis

分别对德国小蠊S品系及R品系血淋巴蛋白进行双向电泳（2D-PAGE），并对电泳图谱进行差异分析。

Two-dimensional electrophoresis ( 2D-PAGE ) was carried out on hemolymph proteins in S strain and R strain of German cockroach and the differences were analyzed .

方法采用2Gy全身照射SD大鼠，分别于辐照前和辐照后24、72h取外周血，分离淋巴细胞，制备蛋白样品，进行双向电泳测定。

Methods the protein samples were extracted from rat peripheral blood lymphocytes 24 h and 72 h after the 2 Gy total-body irradiation .

家蚕基因组完成以后，为了探索30K蛋白的生理功能，我们在全基因组水平上分析了30K蛋白基因家族的构成情况，并利用基因组数据和双向电泳等方法对该类蛋白做了进一步的探索。

Analysis of the genomic data , the results showed ten 30K protein genes are in silkworm .

目的运用双向电泳和基质辅助的激光解吸电离飞行时间质谱（matrix-assistedLaserDesorption/ionizationtime-of-flightMassSpectrometry，MALDI-TOF-MS）的方法，鉴定年龄相关性白内障和正常晶状体之间差异表达的蛋白质。

Objective To identify the different expressed proteins between the normal lens and age-related cataract by two-dimensional gel electrophoresis ( 2-DGE ) and matrix-assisted laser desorption / ionization time-of-flight mass spectrometry ( MALDI-TOF-MS ) .

目的：利用双向电泳技术（2-DE）观察吡咯里西啶生物碱isoline致小鼠急性肝损伤后对肝内亲水性蛋白表达的影响。

AIM : To investigate expression changes of hydrophilic proteins in acute injured mouse liver induced by a pyrrolizidine alkaloid Isoline , using two-dimensional electrophoresis technique .

目前小麦叶片蛋白质组研究主要采用双向电泳（2-DE）分离和肽质量指纹（PMF）分析技术。

Two-dimensional gel electrophoresis ( 2-DE ) and peptide mass fingerprinting ( PMF ) have been the main techniques for the wheat leaf proteome analysis .

分析mRNA差别显示的结果和蛋白质双向电泳结果，发现龙眼在胚性愈伤组织和球形胚阶段，差异表达的mRNA多于后期阶段（子叶形胚和成熟胚阶段），但蛋白质却较后期少。

The obtained results of the mRNA differential display and two-dimensional electrophoresis of the proteins showed that the cDNAs of embryogenic calli and globular embryoids were more than those of cotyledonary embryoids and matured-cotyledonary embryoids , While the proteins were in turn .

光敏核不育水稻农垦58S与农垦58蛋白质双向电泳对比分析

A two dimensional electrophoretic analysis of proteins associated with photoperiod sensitive male sterility in Rice

用双向电泳分离两种细胞蛋白质混合物并进行比较，找出差异点，这些点经过胶内酶切后进行MALDITOF质谱鉴定。

After in_gel protein digestion , the different_expressed spots were detected by matrix_assisted laser desorption ionization_time of flight mass spectrometry ( MALDI TOF MS ) .

野生二粒麦（T.dicoccoides）谷蛋白亚基双向电泳分析及其LMW-GS编码基因的分子克隆

Two-dimensional Electrophoresis of Glutenin Subunits and Molecular Cloning of LMW-GS Coding Genes in T. Dicoccoides

经典的蛋白质组学研究方法包括IEF/SDS-PAGE双向电泳和质谱技术的联用，但由于IEF的一些不足，限制了其应用范围。

The classical approach in proteomic analysis couples IEF / SDS-PAGE with mass spectrum . However , the shortcomings of IEF restrict its widespread applications .