包被抗原

本文以人工合成肽GOR作为包被抗原建立ELISA法检测患者血清中HCV感染相关自身抗体（抗-GOR）。

We synthesized a peptids GOR as antigen for detection of HCV infection-associated autoimmune antibody with ELISA .

试验结果表明，包被抗原1：80（0.0704mg／ml）稀释，待检血清1：40稀释，酶标二抗1：200稀释，是本方法最佳的工作浓度。

The results indicated that diluting the antigen to 0.0704mg/ml and the serum to 1:40 and the marked IgG to 1:200 were the best reaction condition .

结论：采用超滤、PEG沉淀及连续蔗糖密度梯度超速离心的方法可制备满足麻疹IgGEIA诊断试剂要求的包被抗原。

Conclusion : We can prepare the coating antigen in measles IgG EIA test system by continuous sucrose density gradient ultracentrifugation .

目的：利用抗呕吐毒素的特异性单克隆抗体和包被抗原，建立间接竞争酶联免疫法（ELISA）来检测谷物及其制品中呕吐毒素含量。

Objective : An indirect competitive ELISA was developed by using anti-DON monoclonal antibody and coating antigen DON-BSA for detection of deoxynivalenol in wheat and corn .

目的：分析ELISA包被抗原在检测溃疡性结肠炎（UC）血清ACA的非特异性干扰物。

Objective : To analyze the nonspecific interferents in detecting ACA - IgG in sera of patients with UC by ELISA .

分别用碳二亚胺法和混合酸酐法制备诺氟沙星与卵清蛋白（OA）的结合物，作为诺氟沙星酶联免疫测定用的包被抗原。

The conjugates of norfloxacin-ovalbumin ( OA ) used for coating antigen were synthesized by carbodiimide method and mixed anhydride method , respectively .

方法：以细菌抗原作为包被抗原，以酶标IgG作为标记二抗进行IgY药物针对不同病原菌抗体效价的间接ELISA检测。

Methods : Antibody activity of IgY was studied by indirect ELISA with bacteria debris as coating antigen and enzyme-IgG as second antibody .

确定包被抗原浓度、一抗工作稀释度，以此优化条件绘制标准曲线，从而初步建立检测人血清ADAM12-S的间接竞争法ELISA。

Determine the optimal concentration of coating antigen and dilution of the first antibody , a standard curve under these conditions generated to establish indirect competitive ELISA preliminarily .

用NTPBSA致敏明胶颗粒的凝集试验和NTOBSA为包被抗原的ELISA检测了1334份血清，发现1309例的两种试验结果一致，其中120例均为阳性。

Agglutination tests with gelatin particles sensitized by NT P BSA and ELISA with NT O BSA as coating antigen were made in 1334 specimens of serum .

以真核表达的融合蛋白为包被抗原建立了间接ELISA方法，用来检测动物体内FMD的抗体水平。

Indirect ELISA that can detected the valence of antibody was developed with the fusion protein expressed by eukaryotic expression system as antigen .

结果以HCⅡ及BCⅡ作为包被抗原检测RA患者血清抗CⅡ抗体的阳性率分别约为30.0%和33.3%；

Results The positive rates of serum anti-C ⅱ antibodies to HC ⅱ or BC ⅱ in the RA patients were 30.0 % and 33.3 % , respectively ;

采用福尔马林灭活的副猪嗜血杆菌全菌体作为包被抗原，建立了检测副猪嗜血杆菌抗体的间接ELISA方法。

Haemophilus parasuis inactivated with 0.2 % formalin was applied as antigen , and an indirect ELISA was developed by determination of the optimal reaction condition to monitor serum antibody levels in pigs .

方法以纯化重组P24为包被抗原，应用ELISA检测HIV-Ab国家参比品和正常人血清。

Methods National reference for HIV-Ab and normal human sera were detected by ELISA using recombinant p24 as coated antigen .

以GST-Fba重组蛋白为包被抗原，采用间接ELISA方法检测小鼠抗血清效价。

The titer of antiserum was tested by indirect ELISA using GST-Fba recombination protein as the coated antigen .

以原核表达NiVG蛋白片段为包被抗原，间接ELISA检测rWR-NiV-G感染免疫小鼠血清中的G蛋白特异抗体，具有良好的敏感性和特异性。

The prokaryotic expressing G protein fragment showed sensitive and specific antigenic reaction to NiV G protein specific antibody from rWR-NiV-G immunized mice serum in indirect ELISA .

用丙型肝炎病毒（HCV）核心区2个型特异性合成多肽作为包被抗原，建立了ELISA法，对血清中抗－HCV进行分型研究。

We used two synthetic peptides deduced from the putative core protein of hepatitis C virus ( HCV ) to set up the ELISA systems for differentiating the HCV antibodies into two types .

用表达的口蹄疫3A蛋白作为包被抗原，建立了间接ELISA试验，对口蹄疫病毒感染血清、免疫血清和其它非口蹄疫病毒血清进行了检测研究。

An indirect ELISA was established with expressed3A protein of FMDV as coating antigen and was experimentally used to detect FMDV infection sera , immune sera and other disease sera .

结论用酵母菌表达的HSVⅠ重组gD蛋白作为包被抗原进行ELISA检测是一种敏感、特异的方法，具有较大的应用价值。

Conclusion The ELISA with recombinant gD protein of HSV - ⅰ as coating antigen is a specific , sensitive , quick and convenient method for diagnosis of HSV - ⅰ infection .

方法以重组p53蛋白作为包被抗原，检测ELISA方法的敏感性、特异性、精密度与稳定性，同时对203份正常人血清及548份临床确诊恶性肿瘤病人血清进行检测。

Methods The recombinant p53 protein was used as the coating antigen to test sensitivity , specificity , precision and stability of ELISA method . 203 serum samples from healthy individuals and 548 from patients with malign tumor were examined .

为了评价仔猪大肠杆菌病K88-K99-987P-F41四价亚单位疫苗的免疫效果，利用纯化粘附素为包被抗原，建立了检测产肠毒素性大肠杆菌粘附素抗体的间接ELISA。

An indirect ELISA for detecting antibodies to an K88-K99-987P-F41 subunit vaccine against piglet colibacillosis was established by using purified adhesions for coating antigens .

鸡卵黄抗体的最佳ELISA操作参数为：包被抗原浓度为7.5μg/ml，封阻剂浓度为2.5%，抗体稀释倍数为1000，酶标二抗稀释倍数为14000；

The optimum ELISA parameters with IgY were that the concentration of antigen and blocking agent was 7.5 μ g / ml , 2.5 % and the dilution multiples of IgY and anti-chicken rabbit antibody-HRP were 1000 and 14000 , respectively .

用卵清白蛋白与伏马菌素B2的偶联物（OVA-FB2）做包被抗原，标准伏马菌素B2（FB2）做竞争抗原，初步建立了检测玉米粉中伏马菌素B2的间接竞争ELISA方法。

An ic-ELISA for detecting fumonisin B2 ( FB2 ) of maizena was developed by using the coating antigen of OVA-FB2 and competitive antigen of FB2 .

用TMEV和痘苗病毒感染BHK-21细胞，制备病毒特异抗原，确定包被抗原和标准阳性血清的最佳工作浓度；并进行敏感性、特异性、重复性和稳定性实验。

BHK-21 cell line was infected by TMEV and Vaccinia virus to prepare specific virus antigen .

应用赤羽病病毒（AKV）OBE1株和牛标准阴阳性血清，以蔗糖密度梯度离心纯化细胞毒为包被抗原，在国内首次建立了检测AKV抗体的间接ELISA方法。

With the OBE-1 strain and the standard serum , the indirected ELISA has been developed for detecting antibody of Akabane virus in serum .

以GST-Aβ42为包被抗原，间接ELISA法检测Aβ抗体灵敏度为2ng/ml；

The sensitivity of the indirect ELISA for detecting A β antibody was about 2 ng / ml using GST-A β 42 fusion protein as the coating antigen .

以蔗糖密度梯度离心法纯化的病毒作为包被抗原，建立了检测鸭肝炎病毒（DHV）抗体的间接ELISA方法。

An indirect enzyme linked immunosorbent assay ( ELISA ) is developed for detection of antibodies against duck hepatitis virus ( DHV ) in ducks . The coating antigen was purified with sucrose density gradients centrifugation .

以纯化到的N蛋白作为包被抗原建立了间接ELISA方法（N-ELISA），可以检测出鸡血清中的抗IBVN蛋白的特异性抗体。

With the purified N protein as coating antigen , an indirect ELISA assays ( N-ELISA ) were established which could detect the antibody in chicken serum against N protein of IBV . The reaction conditions of N-ELISA were optimized .

方法ELISA法以PPD为包被抗原，每隔3个月检测治愈时血清抗结核抗体呈阳性的肺结核病人血清抗结核抗体1次，连续18个月。

Methods The anti TB IgG in the serum in cured tuberculosis patients had been tested by ELISA in which PPD acts as the coated antigen every three months for 18 months . Results The number of positive patients decreased gradully .

建立以38kD多肽为包被抗原的DIGFA（P38-DIGFA），并检测147例临床确诊的活动性肺结核病人血清标本。

The P38-DIGFA assay was used to detect the specific antibodies in serum samples of 147 cases of patients with active tuberculosis .

采用混合酸酐法将SDL与人血清白蛋白（HSA）连接制备了免疫抗原SDLHSA，用同样的方法将其与卵清白蛋白（OVA）连接制备了包被抗原SDLOVA。

Immunizing antigen ( SDL HSA ) and coating antigen ( SDL OVA ) were synthesized by conjugating SDL to human serum albumin ( HSA ) and ovalbumin ( OVA ) with the same method of mixed anhydride .