亲和素

  • 网络Avidin;HRP-avidin
亲和素亲和素
  1. 方法:直接包被法、SPA法、生物素-亲和素法。

    Method : Direct method , SPA method , Biotin Avidin method .

  2. 另外,测得ABP与亲和素结合的结合常数为1.4×10~(-7)mol/L。

    The constant of combination of ABP and avidin is 1 . 4 10  ̄( - 7 ) M  ̄( - 1 ) .

  3. 亲和素-生物素间接偶联的压电DNA传感器研究

    The Studies on Biotin-avidin Indirect Conjugated Technology for Piezoelectric DNA Sensor

  4. 生物素&亲和素系统检测食品中黄曲霉毒素B1的研究

    Investigation on the detection of aflatoxin B-1 with ABC-ELISA

  5. 生物素-亲和素系统在MR靶向成像中的应用

    Application of biotin-avidin system in targeted magnetic resonance imaging

  6. 通过选择最佳的链霉亲和素浓度和生物素化DNA的浓度,控制了非特异性扩增。

    We controlled the nonspecific amplification using the optimal concentration of DNA label and streptavidin .

  7. 人白细胞环孢素A亲和素mRNA表达水平的检测

    The Expression of Cyclophilin mRNA in the Human White Blood Cells

  8. 亲和素-生物素系统(Avidim-Biotinsystem,ABS)已广泛用于免疫标记检测技术。

    A new type , biological magnified reaction system Avidin-Biotin System ( ABS ) have been widely using in immunoassay .

  9. 方法利用亲和素作为桥梁连接生物素化DNA和抗体,形成抗体亲和素DNA偶联物。

    Methods Biotinylated DNA and antibody were coupled with avidin , thus an antibody avidin DNA conjugate was prepared .

  10. 固相生物素-亲和素ELISA检测HIV抗体方法的建立与应用

    The Establishment and Application Of Solid Phase Biotin Avidin ELISA Determinating HIV Antibodies

  11. 血池对比剂Gd-DTPA-链霉亲和素对裸鼠肝癌模型的MR增强显像研究

    Contrast - enhanced MR imaging of nude mice grafted with hepatocellular carcinoma using blood pool contrast agent Gd - DTPA - streptavidin

  12. 链酶亲和素-生物素ELISA检测人心肌肌钙蛋白T

    Streptavidin-biotin ELISA for human cardiac troponin T

  13. 应用生物素-亲和素系统检测胸水特异性IgG抗体的研究

    The Detection of Specific IgG Antibody in the Pleural Fluids with ABC - ELISA

  14. 且固定化寡核苷酸探针保持了较好的生物学活性,所制备链亲和素磁性微粒在免疫检测、PCR产物纯化、特定mRNA纯化方面都获得了较好的结果。

    Good experimental results of Streptavidin in the application of immunoassay , PCR products purification and mRNA purification were obtained .

  15. 目的探讨利用生物素-亲和素系统的靶向定位效应和生物放大作用,提高MR分子成像的敏感性。

    Objective To improve the sensitivity of magnetic resonance molecular ( MR ) imaging on the basis of the targeting and magnifying effects of biotin-avidin system .

  16. 目的研制亲和素生物素酶联免疫吸附法(ABCELISA)定量检测人表皮生长因子(EGF)试剂盒。

    Objective To establish ABC enzyme-linked immunosorbent assay ( ABC ELISA ) for quantitatively detecting the human EGF .

  17. 应用链霉亲和素在ELISA中检测HBe系统

    Application of Streptavidin in ELISA for Detection of Hepatitis Be Antigen and Antibody

  18. 人尿中IgG的时间分辨荧光免疫分析法&以铕标记链亲和素作为标记物

    A Time-resolved Fluoroimmunoassay for Determination of Human Immunoglobulin ( IgG ) in Urine-Streptavidin-Eu (ⅲ) as a label in immunoassay

  19. 以光敏生物素-金标链亲和素核酸原位杂交及免疫组化方法检测膀胱癌病理标本中P53基因片段及其蛋白表达。

    With nucleic acid in situ hybridization and immunohistochemical methods , the p53 gene fragment and its protein expression were determined .

  20. 采用生物素-亲和素双抗体夹心酶联免疫吸附分析技术(ABC-ELISA)检测了哮喘患儿血浆和淋巴细胞诱生γ-干扰素(IFN-γ)水平。

    IFN - γ level in children wiht asthma was examined by using avidin-biotin complex sandwich enzyme immunoassay ( ABC-ELISA ) .

  21. 方法:采用链酶亲和素-过氧化物酶标记免疫组织化学方法,在PCNA阳性标本中测定增殖指数(PI)。

    Methods The PCNA index ( PI ) was detected by the immunohistochemistry method of streptavidin in the PCNA positive samples .

  22. 方法应用亲和素生物素酶复合物技术(ABC法)观察UU感染者,并与培养法和PCR法相比较。

    80 clinical specimens were examined by avidin biotin ? peroxidase complex technique ( ABC technique ), PCR method and cultivation .

  23. 洗涤后,加亲和素-生物素化HRP复合物(ABC),继加底物溶液。

    After washing , avidin-biotinyl HEP complex ( ABC ) was added , followed by the addition of substrate solution .

  24. PCR扩增被检标本中目的片段,其引物用Bio-Ⅱ-dUTP修饰,在微孔板上杂交后用链霉亲和素碱性磷酸酶系统显色。

    Objective fragment of examined sample was amplified by PCR and its primer modified with Bio - ⅱ - dUTP was colored by streptavidin ALP system after hybridization .

  25. 方法将生物素化等位基因特异性寡核苷酸固定于生物芯片上长链烷烃硫醇自组装单层已连接链霉亲和素(SA)的部位。

    Methods Allele specific biotinylated oligonucleotides were immobilized onto defined areas of streptavidin tethered self assembled monolayers of long chain alkane thiols on biochips .

  26. 本研究分别建立了检测玉米中ZEN的化学发光酶免疫分析法及生物素-亲和素酶联免疫分析法。

    In this study , chemiluminescence analysis and biotin-avidin system were used to establish CLEIA and BA-ELISA for detecting ZEN in corn .

  27. 方法:将聚苯乙烯微孔板(PS)经过紫外线(UV)辐照后,用葡萄球菌A蛋白(SPA)预包被,再应用生物素-链霉亲和素(BSA)反应建立双抗体夹心ELISA。

    Methods Precoating staphylococcal protein A ( SPA ) on UV-irradiated PS plate , then applying biotin-Streptavidin system to establish a kind of sandwich ELISA method .

  28. 前曾发现在日本血吸虫虫卵中,存在一种新的能与亲和素专一性结合的蛋白质,称为亲和素结合蛋白(ABP)。

    We have found an Avidin-binding protein ( ABP ) in the eggs of Schistosoma japonicum .

  29. 应用生物素亲和素酶联免疫吸附法(BA-ELISA)测定了36例喉癌病人血清谷胱甘肽S转移酶(GST)的含量。

    The levels of serum glutathione S-transferase ( GST ) in 36 patients with laryngeal carcinoma were measured by enzyme linked immunosorbent assay ( ELISA ) .

  30. ISSR-PCR和链亲和素磁珠吸附法开发白菜SSR引物

    SSR Primers Development in Non - heading Chinese Cabbage by ISSR - PCR and Streptavidin - coated Magnetic Beads Adsorption