免疫荧光
- 名immunofluorescence
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方法:选用14~16种单克隆抗体,采用免疫荧光的方法,对96例AL患者进行免疫分型。
Method : 96 patients with AL were investigated with MoAb and immunofluorescence .
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以间接免疫荧光和Western印迹检测细胞色素C在细胞内的分布。
Membrane potential was determined with JC-1 . Cellular distribution of cytochrome C was detected with either immunofluorescence or Western blot .
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用间接免疫荧光技术诊断柯萨奇B组病毒中枢神经系统感染
Diagnosis of Coxsakie B Virus Infection in Central Nervous System by Indirect Fluorescent Antibody Assay
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微量免疫荧光试验和PCR在诊断肺炎衣原体急性感染中的应用
Application of microimmunofluorescence test and PCR in the diagnosis of acute Chlamydia pneumoniae infections
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定量PCR和间接免疫荧光法联合检测生殖器疱疹病毒感染
Combined Detection for Herpes Simplex Virus of Genital Herpes with Quantity PCR and Indirect Immunofluorescence
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ELISA和免疫荧光法检测甲状腺自身抗体
Detection of thyroid auto - antibody with ELISA and immuno - fluorescence assay
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免疫荧光结果与PCR相一致。
The immunofluorescence confirmed this result .
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间接免疫荧光试验检测登革IgG的应用
Application of Indirect Immunofluorescent Assay for Detecting IgG of Dengue Virus
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免疫荧光法检测TGFβ1蛋白表达。
The expression of TGF - β _1 was detected by immunofluorescence staining .
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间接免疫荧光试验在人群埃可30型肠道病毒IgG抗体检测中的应用
Preliminary application of indirect immunofluorescence assay for detecting IgG antibodies to Echovirus 30
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目的研制人C肽(Cpeptide)时间分辨免疫荧光分析(TRFIA)试剂盒。
Objective To prepare a time-resolved fluoroimmunoassay ( TRFIA ) kit for detection of human C-peptide .
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间接免疫荧光标记流式细胞术检测移植瘤血管内皮生长因子(VEGF)荧光峰值的表达情况;
The expression of VEGF in xenograft tumor cells was analyzed by indirect immunofluorescence flow cytometry .
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应用间接免疫荧光法、免疫印迹法证实了在453例MM患者血清内有大量的自身抗体(阳性率达41.04%)。
The positive rate was 41.04 percent in 453 MM cases by the use of indirect immunofluorescence and Western blotting .
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小儿IgA肾病免疫荧光表现及其意义
Significance of immunofluorescence findings in children with IgA nephropathy
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方法以牛舌粘膜作为底物,分别应用间接免疫荧光法和免疫印迹技术检测16例天疱疮患者血清中IgG型自身抗体。
Methods To detect autoantibodies in 16 cases of sera from patients with pemphigus by immunofluorescence .
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免疫荧光细胞化学技术及半定量分析心肌细胞促凋亡基因c-fos的蛋白表达。
Cfos protein in cardiomyocytes was detected using semiquantitative immunofluorescence cytochemistry technique .
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应用DTZ染色、Insulin免疫荧光染色对诱导的细胞进行鉴定。
Differentiated cells were detected by DTZ staining and Insulin immunofluorescence staining .
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利用间接免疫荧光标记和Western印迹方法分析蛋白激酶C活性对锚蛋白及CD44的亚细胞分布及蛋白质表达的影响。
Effects of PKC activity on subcellular distribution and protein expression of ankyrin and CD44 was analyzed respectively with indirect immunofluorescence and Western blot .
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使用间接免疫荧光法对71例急性白血病(AL)患者的白血病细胞进行了免疫表型分析。
Immunophenotypic features of71 patients with acute leukemia ( AL ) were analysed by a standard indirect immunofluorescence assay using a panel of18 monoclonal antibodies .
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免疫荧光染色及RT-PCR检测,发现其表达多能性细胞及生殖细胞的标记,而且具有增殖能力,能够向三个胚层的细胞分化。
Immunofluorescence staining and RT-PCR determination found that the cells express pluripotency , proliferation and germ cells ' markers .
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MTT方法检测细胞活力,免疫荧光细胞化学染色观察细胞内包涵体形成的变化。
Cell vitality was measured by MTT assay . The formation of inclusions was determined with immunofluorescence cytochemistry staining .
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间接免疫荧光试验证明:该组单抗测得不同人群外周血淋巴细胞阳性数与用抗B细胞单抗和直接免疫荧光法测得的结果接近。
An approximate positive rate of peripheral blood lymphocyte ( PBL ) was achieved when these McAbs and the McAb against B lymphocytes were tested by indirect immunofluorescent assay ( IFA ) .
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间接免疫荧光(IFA)鉴定结果表明:F和HN基因均能在细胞中得到较好的表达;
The results of IFA showed that F and HN gene expressed well in COS-1 cell .
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通过免疫荧光细胞化学法检测神经纤丝(NF)的表达,进行神经元鉴定及纯度计算;
Neurofilament ( NF ) was detected by immunofluorescence staining to identify neurons and to calculate the purity of neurons .
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方法收集乙型肝炎患者695例,时间分辨免疫荧光测定患者血清乙型肝炎e抗原(HBeAg),同时荧光定量PCR测定患者血清HBV-DNA。
Methods Serum HBeAg and HBV-DNA in 695 infected persons were detected by time resolved fluorescence immunoassay and fluorescence quantitative PCR .
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间接免疫荧光法和原位杂交法检测急性白血病中Bcl-2和Bcl-2/Bax表达的对比研究
Comparision between indirect immunofluorescence assay and in situ hybridization assay in detecting the expression of Bcl-2 and Bcl-2 / Bax in acute leukemia
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方法用免疫荧光技术检测48例胶质瘤患者围手术期T淋巴细胞亚群和NK细胞百分率。
Methods The positive percentage of T lymphocyte phenotype and NK cell were examined dynamically by method of direct immunofluorence .
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免疫荧光及RT-PCR检测心肌细胞特异性标志物。
Immunocytochemistry staining and RT-PCR indicated that the cells derived from ES cells were cardiomyocytes based on the presence of cardiac-specific molecular marker .
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方法免疫荧光法,糖原试验,胶体金标记技术免疫检测,聚合酶链反应(PCR)分别对100例妇产科就诊患者宫颈分泌物标本进行平行检测。
Methods Immunofluorescence , glycogen test , gold labeling immunoassay and polymerase chain reaction ( PCR ) were used to detect specimen of cervical secretion from 100 women .
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方法:用流式细胞仪根据直接免疫荧光法分别测定病例组与对照组T细胞表面标志CD3,CD4,CD8的表达情况。
Methods : CD 3 , CD 4 and CD 8 were labeled by immunofluorescence , determined by flow cytometry .