免疫荧光

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  • immunofluorescence
免疫荧光免疫荧光
  1. 方法:选用14~16种单克隆抗体,采用免疫荧光的方法,对96例AL患者进行免疫分型。

    Method : 96 patients with AL were investigated with MoAb and immunofluorescence .

  2. 以间接免疫荧光和Western印迹检测细胞色素C在细胞内的分布。

    Membrane potential was determined with JC-1 . Cellular distribution of cytochrome C was detected with either immunofluorescence or Western blot .

  3. 用间接免疫荧光技术诊断柯萨奇B组病毒中枢神经系统感染

    Diagnosis of Coxsakie B Virus Infection in Central Nervous System by Indirect Fluorescent Antibody Assay

  4. 微量免疫荧光试验和PCR在诊断肺炎衣原体急性感染中的应用

    Application of microimmunofluorescence test and PCR in the diagnosis of acute Chlamydia pneumoniae infections

  5. 定量PCR和间接免疫荧光法联合检测生殖器疱疹病毒感染

    Combined Detection for Herpes Simplex Virus of Genital Herpes with Quantity PCR and Indirect Immunofluorescence

  6. ELISA和免疫荧光法检测甲状腺自身抗体

    Detection of thyroid auto - antibody with ELISA and immuno - fluorescence assay

  7. 免疫荧光结果与PCR相一致。

    The immunofluorescence confirmed this result .

  8. 间接免疫荧光试验检测登革IgG的应用

    Application of Indirect Immunofluorescent Assay for Detecting IgG of Dengue Virus

  9. 免疫荧光法检测TGFβ1蛋白表达。

    The expression of TGF - β _1 was detected by immunofluorescence staining .

  10. 间接免疫荧光试验在人群埃可30型肠道病毒IgG抗体检测中的应用

    Preliminary application of indirect immunofluorescence assay for detecting IgG antibodies to Echovirus 30

  11. 目的研制人C肽(Cpeptide)时间分辨免疫荧光分析(TRFIA)试剂盒。

    Objective To prepare a time-resolved fluoroimmunoassay ( TRFIA ) kit for detection of human C-peptide .

  12. 间接免疫荧光标记流式细胞术检测移植瘤血管内皮生长因子(VEGF)荧光峰值的表达情况;

    The expression of VEGF in xenograft tumor cells was analyzed by indirect immunofluorescence flow cytometry .

  13. 应用间接免疫荧光法、免疫印迹法证实了在453例MM患者血清内有大量的自身抗体(阳性率达41.04%)。

    The positive rate was 41.04 percent in 453 MM cases by the use of indirect immunofluorescence and Western blotting .

  14. 小儿IgA肾病免疫荧光表现及其意义

    Significance of immunofluorescence findings in children with IgA nephropathy

  15. 方法以牛舌粘膜作为底物,分别应用间接免疫荧光法和免疫印迹技术检测16例天疱疮患者血清中IgG型自身抗体。

    Methods To detect autoantibodies in 16 cases of sera from patients with pemphigus by immunofluorescence .

  16. 免疫荧光细胞化学技术及半定量分析心肌细胞促凋亡基因c-fos的蛋白表达。

    Cfos protein in cardiomyocytes was detected using semiquantitative immunofluorescence cytochemistry technique .

  17. 应用DTZ染色、Insulin免疫荧光染色对诱导的细胞进行鉴定。

    Differentiated cells were detected by DTZ staining and Insulin immunofluorescence staining .

  18. 利用间接免疫荧光标记和Western印迹方法分析蛋白激酶C活性对锚蛋白及CD44的亚细胞分布及蛋白质表达的影响。

    Effects of PKC activity on subcellular distribution and protein expression of ankyrin and CD44 was analyzed respectively with indirect immunofluorescence and Western blot .

  19. 使用间接免疫荧光法对71例急性白血病(AL)患者的白血病细胞进行了免疫表型分析。

    Immunophenotypic features of71 patients with acute leukemia ( AL ) were analysed by a standard indirect immunofluorescence assay using a panel of18 monoclonal antibodies .

  20. 免疫荧光染色及RT-PCR检测,发现其表达多能性细胞及生殖细胞的标记,而且具有增殖能力,能够向三个胚层的细胞分化。

    Immunofluorescence staining and RT-PCR determination found that the cells express pluripotency , proliferation and germ cells ' markers .

  21. MTT方法检测细胞活力,免疫荧光细胞化学染色观察细胞内包涵体形成的变化。

    Cell vitality was measured by MTT assay . The formation of inclusions was determined with immunofluorescence cytochemistry staining .

  22. 间接免疫荧光试验证明:该组单抗测得不同人群外周血淋巴细胞阳性数与用抗B细胞单抗和直接免疫荧光法测得的结果接近。

    An approximate positive rate of peripheral blood lymphocyte ( PBL ) was achieved when these McAbs and the McAb against B lymphocytes were tested by indirect immunofluorescent assay ( IFA ) .

  23. 间接免疫荧光(IFA)鉴定结果表明:F和HN基因均能在细胞中得到较好的表达;

    The results of IFA showed that F and HN gene expressed well in COS-1 cell .

  24. 通过免疫荧光细胞化学法检测神经纤丝(NF)的表达,进行神经元鉴定及纯度计算;

    Neurofilament ( NF ) was detected by immunofluorescence staining to identify neurons and to calculate the purity of neurons .

  25. 方法收集乙型肝炎患者695例,时间分辨免疫荧光测定患者血清乙型肝炎e抗原(HBeAg),同时荧光定量PCR测定患者血清HBV-DNA。

    Methods Serum HBeAg and HBV-DNA in 695 infected persons were detected by time resolved fluorescence immunoassay and fluorescence quantitative PCR .

  26. 间接免疫荧光法和原位杂交法检测急性白血病中Bcl-2和Bcl-2/Bax表达的对比研究

    Comparision between indirect immunofluorescence assay and in situ hybridization assay in detecting the expression of Bcl-2 and Bcl-2 / Bax in acute leukemia

  27. 方法用免疫荧光技术检测48例胶质瘤患者围手术期T淋巴细胞亚群和NK细胞百分率。

    Methods The positive percentage of T lymphocyte phenotype and NK cell were examined dynamically by method of direct immunofluorence .

  28. 免疫荧光及RT-PCR检测心肌细胞特异性标志物。

    Immunocytochemistry staining and RT-PCR indicated that the cells derived from ES cells were cardiomyocytes based on the presence of cardiac-specific molecular marker .

  29. 方法免疫荧光法,糖原试验,胶体金标记技术免疫检测,聚合酶链反应(PCR)分别对100例妇产科就诊患者宫颈分泌物标本进行平行检测。

    Methods Immunofluorescence , glycogen test , gold labeling immunoassay and polymerase chain reaction ( PCR ) were used to detect specimen of cervical secretion from 100 women .

  30. 方法:用流式细胞仪根据直接免疫荧光法分别测定病例组与对照组T细胞表面标志CD3,CD4,CD8的表达情况。

    Methods : CD 3 , CD 4 and CD 8 were labeled by immunofluorescence , determined by flow cytometry .