免疫动物

用westernblot实验验证表达蛋白的抗原性，用免疫动物实验验证其免疫原性。

Antigenicity of the expressed protein was confirmed by Western Blot . Immunogenicity of the expressed protein was confirmed by immune animal .

人用H5N1禽流感疫苗免疫动物效果观察

Observation on effect of avian influenza ( H5N1 ) vaccine with immunization animals

应用该法对小鼠血清的SS抗体检测表明，激生N八和N乃茵免疫动物后，动物产生了一定量的SS抗体，且SS抗体可以维持3－4个月。

The anti-SS test proved immunized animals was induced to produce SS antibody , and SS antibody could last 3-4 months .

DNA疫苗又叫核酸疫苗、基因疫苗，是将编码某种抗原的基因片段克隆到真核表达质粒，再用重组质粒DNA免疫动物。

DNA vaccines , also named as nucleic vaccine or genetic vaccine , which carry exogenous genes under the control of eukaryotic promoters , can be used to immunize animals .

生长抑素基因重组痘苗病毒（VV／SS）作为活载体苗免疫动物，可促进动物生长。

Active immunization against genetically engineering live vector vaccine of somatostatin can promote animal rapid growing .

重组CB免疫动物后，可获得高效价的CB多克隆抗体，用以制备相应的固化抗体和酶标抗体检测CB。

When purified CB was used to immunize the rabbits , high titer polyclonal antibodies could be gained .

部分肝癌疫苗免疫动物前经60Co照射；

A part of the hepatoma vaccine cells was irradiated with 60 Co before immunization .

通过其紫外扫描光谱、SDS-PAGE凝胶电泳试验，表明半抗原和载体成功偶联，免疫动物以后可以产生满意效价。

UV scanning spectrum , SDS-PAGE and animal immunity all indicated that the complete antigens both SMM-BSA and SMM-OVA were successfully produced .

结论利用GSTPrP融合蛋白可有效地剌激免疫动物产生PrP特异性抗体，所制备的抗血清可试用于朊蛋白的检测。

Conclusion The prokaryotic expressed GST PrP fusion protein can efficiently elicit in immunized animals the PrP specific antibody .

对用于检测诊断的PCR方法进行了优化，同时对采集于疫苗免疫动物群的样品中犬瘟热病毒和狐狸脑炎病毒的核酸进行了特异性检测；

Optimized the PCR protocol for detection and diagnosis , and at the same time give specific detection on nucleic acid in CDV and Fox Encephalitis virus from samples collected from vaccinated animals .

同时，实验提示采用体外诱导源于骨髓的APC加强抗原提呈，可以提高免疫动物对低免疫原性抗原的反应性。

Our experiments suggested that the low immunogenicity can be altered by antigen presenting process with homogenous APC induced from bone marrow in vitro .

方法用肝微粒体制备抗原，免疫动物制备抗体，并用免疫沉淀、固相免疫吸附、WESTERNBLOTTING法鉴定抗体特性。

Methods The antibodies were obtained by antigen immunizing animals , the antigen was prepared from liver microsome , the speciality of the antibody was identified by the methods of immunosedimentation and Western blotting .

并用重组抗原和真核重组DNA质粒分别免疫动物，观察其诱导产生的特异性免疫应答反应，探讨其作用机制，并通过对鸡只攻击E.tenella的保护作用检测其抗球虫效果。

In order to observe the induced specific immune response , the recombinant antigen and eukaryotic recombinant DNA plasmids were immunized animals to gain the protective effect .

抗体通常是从免疫动物血液中获得的，卵黄抗体（IgY）指从免疫禽蛋中提取出的针对特定抗原的抗体。

Antibodies are usually produced from immunized animal plasma . Egg yolk antibody ( IgY ) against specific antigens are extracted from immunized birds .

14a细胞（1×107/鼠），再用50μg/鼠PLP136150修饰抗原免疫动物，以触发被动实验性变态反应性脑脊髓炎（EAE）。

Then mice were immunized with 50 μ g / mouse APL for inducing passive experiment allergic encephalomyelitis ( EAE ) .

用提取的重组噬菌体免疫动物，六周后经ELISA检测，各种重组噬菌体都能诱导针对相应B细胞表位的抗体产生，且特异性良好。

The recombinant T7 phages were extracted and used to immunize mice , 6 weeks later , the anti-sera was analyzed by ELISA . The results indicated that all the recombinant phages induced the generation of specific antibody against the respective B cell epitope .

提示ZP免疫动物的抗生育作用，与卵巢功能受抑制有关。

These results show that the antifertility effect of immunization by porcine ZP , no matter iso-or hetero , is related to its inhibition of ovarian function .

目的为提高口蹄疫诊断试剂的敏感性和特异性，拓宽试剂的使用范围，用非结构蛋白研制重组抗原和高免血清，建立鉴别口蹄疫免疫动物与感染动物的竞争ELISA方法。

Object In order to enhance the specificity and sensitivity of diagnostic reagents for FMD , a recombinant antigen and hyperimmune serum were developed with NSPs ( 3ABC ) for the competitive ELISA kit to distinguish the infected animals from vaccinated and FMDV free animals .

结论：构建成功双启动子表达质粒pCN-SSIE，在体外真核和原核细胞中均能正常表达，用其作为DNA疫苗免疫动物，可诱导明显的免疫应答。

CONCLUSION : The construction of the dual-promoter expression plasmid pCN-SSIE was successful and the plasmid can express in prokaryocyte and eukaryocyte and elicit dramatic immune response when applied as DNA vaccine in experimental animal .

紫外扫描分析表明，T-2毒素与牛血清白蛋白的偶联比为6.66∶1、与卵清蛋白的偶联比为10.11∶1，表明此完全抗原能够用于免疫动物。

UV Spectrum analysis showed that the conjugation ratio of T-2 toxin to BSA was6.66 ∶ 1 and that to OVA was10.11 ∶ 1 , indicating this antigen could be used to immunize animals .

通过优化抗原包被浓度、封闭液和金标SPA工作浓度等建立了能够区分口蹄疫自然感染动物和免疫动物的斑点免疫金渗滤法和胶体金免疫层析试纸法。

The research established DIGFA and GICA witch could differentiate the natural infected animals from vaccinated animals by the way of optimizing the concentration of antigen coated on the NC film , the confining liquid and the concentration of colloidal gold labelled with SPA .

原核表达的GST-BoPrP（23～242）融合蛋白能有效地刺激免疫动物产生PrP特异性抗体，所制备的抗血清可适用于天然朊蛋白的检测。

The GST-PrP ( 23 ~ 242 ) fusion protein could efficiently stimulate the immunized animals to produce the PrP-specified antibody , and the antiserum could be used for detecting the native prion proteins .

研究结果初步表明，采用人IgE分子受体结合部位的适当残基序列的合成肽疫苗，免疫动物所诱导的抗体可抑制Ⅰ型变态反应。

The preliminary results indicate that the antisera , which were induced by IgE peptide vaccines derived from proper sequences of receptor binding sites on IgE molecule , can suppress hypersensitivity , showing the potentiality of IgE peptide vaccine as a general treatment preparation for Type I hypersensitivity .

结论利用原核细胞表达的GST1433蛋白可有效地诱导免疫动物产生特异性抗体，所制备的抗体可用于CJD病人脑脊液1433蛋白的检测。

Conclusion Using purified prokaryotic expressed GST 14 3 3 fusion protein as antigen , the specific antibody was elicited in the immunized animals . The prepared antibody can be used in identification of 14 3 3 protein in CSF for the diagnosis of CJD .

用计算机软件预测抗原决定簇来设计多肽，通过合成多肽免疫动物制备抗体，检测水稻在不同发育时期的表达谱，对U-box蛋白质的表达水平进行分析。

Epitope prediction were carried out by computer software to synthesize polypeptides . Polyclonal antibodies were generated by rabbit immunization . Western blotting analysis were carried out for rice material collected at different developmental stages to detect expression pattern .

鉴别布鲁菌病自然感染动物与疫苗免疫动物胶体金检测试纸条的研制

Development of Colloidal Gold Test Strip-discern Infected and Vaccinated Animals by Brucella

通过观察免疫动物的生存期，免疫荧光法检测肿瘤血管密度，评价重组疫苗菌的抗血管及肿瘤生长抑制作用。

The survival was observed and vessel density was measured by immunofluorescence .

用间接血凝实验检测免疫动物血清中的破伤风抗体单位。

Detect the tetanus antiserum units of immunized animals by indirect hemagglutination assay .

一次脾内直接注射免疫动物制备单克隆抗体

Intrasplenic primary immunization for the production of monoclonal antibodies

缓释伤寒荚膜多糖微球疫苗免疫动物的实验研究

Experimental Study of Controlled Release Microencapsulated Samonella Typhi Capsular Polysaccharide Vaccines Immunized Mice