免疫荧光法

miǎn yì yínɡ ɡuānɡ fǎ
  • immunofluorescence method
免疫荧光法免疫荧光法
  1. 蛋白印迹法与免疫荧光法检测白血病患者Survivin表达

    Expression of Survivin in leukemia by Western blot analysis and immunofluorescence method

  2. 结果:①间接免疫荧光法检测P53蛋白:P53蛋白表达在细胞核内,转染组和非转染组细胞中均有P53蛋白表达,转染组细胞内P53蛋白表达明显高于非转染组细胞。

    RESULTS ① Detection of P53 protein with indirect immunofluorescence method P53 protein was expressed in the nuclear of fibroblasts .

  3. 用间接免疫荧光法和PCR技术重新评价抗CMV-抗体检测的临床意义

    Detection of Cytomegalovirus by Indirect Immunofluorescence Assay and PCR Compared with Cytomegalovirus Antibody Test

  4. 免疫荧光法检测p53蛋白定位表达。

    The expression of p53 was detected by immunological fluorescence assay .

  5. 双重染色免疫荧光法评价选择性IgA缺乏症的B细胞成熟度

    Evaluation of maturity of B cells in selective IgA deficiency by double immunofluorescent study

  6. 定量PCR和间接免疫荧光法联合检测生殖器疱疹病毒感染

    Combined Detection for Herpes Simplex Virus of Genital Herpes with Quantity PCR and Indirect Immunofluorescence

  7. ELISA和免疫荧光法检测甲状腺自身抗体

    Detection of thyroid auto - antibody with ELISA and immuno - fluorescence assay

  8. 间接免疫荧光法检测肝活检组织中HCV抗原

    Detection of HCV antigen in liver tissue with indirect immunofluorescence technic

  9. 免疫荧光法检测TGFβ1蛋白表达。

    The expression of TGF - β _1 was detected by immunofluorescence staining .

  10. 免疫荧光法与p53单链构象多态性分析检测胸水肿瘤细胞的结果分析

    Analysis of tumor cells in thoracic liquid by fluorescence and p53 single-strand conformation polymorphism

  11. 时间分辨免疫荧光法和电化学发光法检测HBsAg的对比分析

    Comparative analysis of HBsAg detection by time-resolved immunofluorescence assay and electrochemiluminescence assay

  12. 将构建的重组质粒DNA分别转染真核细胞后用免疫荧光法可检测到外源蛋白的表达。

    Then these recombinant plasmid DNAs were transfected into eukaryotic cells , respectively . The foreign proteins were expressed and detected by IFA .

  13. 间接免疫荧光法检测子宫内膜IgG抗体诊断子宫内膜异位症

    The diagnosis value of endometrial IgG antibodies with immunofluorescence assay in patients with endometriosis

  14. 应用间接免疫荧光法、免疫印迹法证实了在453例MM患者血清内有大量的自身抗体(阳性率达41.04%)。

    The positive rate was 41.04 percent in 453 MM cases by the use of indirect immunofluorescence and Western blotting .

  15. 间接免疫荧光法在检测SARS冠状病毒特异性抗体中的应用

    IFA in testing specific antibody of SARS coronavirus

  16. 经活细胞免疫荧光法和细胞ELISA法测定,免疫导向药物保持了抗体活性。

    The conjugates were proven keeping the original immunological activity with living cell immunofluorescence test and cell ELISA .

  17. 方法以牛舌粘膜作为底物,分别应用间接免疫荧光法和免疫印迹技术检测16例天疱疮患者血清中IgG型自身抗体。

    Methods To detect autoantibodies in 16 cases of sera from patients with pemphigus by immunofluorescence .

  18. 重组腺病毒感染哺乳动物细胞后,用间接免疫荧光法和westernblot法检测重组蛋白在细胞中的表达情况,结果显示,重组腺病毒感染哺乳动物细胞后能够表达目的蛋白。

    The results of indirect immunofluorescent assay and Western blot indicated that the chimeric adenoviruses could express the recombinant protein in BHK-21 cells .

  19. 使用间接免疫荧光法对71例急性白血病(AL)患者的白血病细胞进行了免疫表型分析。

    Immunophenotypic features of71 patients with acute leukemia ( AL ) were analysed by a standard indirect immunofluorescence assay using a panel of18 monoclonal antibodies .

  20. 注射ISF后第7天、第12天,分别用单克隆抗体免疫荧光法检测小鼠脾中的B细胞、CD4和CD_8 ̄+细胞。

    And 12 days later , B cell , and Tcells were detected by monoclonal antibody immunofluorescence methods .

  21. 抗PML抗体免疫荧光法快速诊断急性早幼粒细胞白血病

    Rapid diagnosis of acute promyelocytic leukemia by anti-PML antibody immunofluorescence assay

  22. 将获得的多克隆抗体,用ELISA与免疫荧光法,研究其特异性。

    Make research on the specificity of polyclonal antibody , using the methods of ELISA and indirect immunofluorescence ( IFA ) . 3 .

  23. 免疫荧光法测定血清总睾酮(TT)、雌二醇(E2)、孕酮(P)水平。

    Serum total testosterone ( TT ), estradiol ( E_2 ) and progesterone ( P ) levels were determined by immunofluorescence assay .

  24. 并发现DIG标记的DNA探针检测标本与细胞培养法具有相似的敏感性和特异性,高于免疫荧光法。

    We found the sensitivity and specificity of DNA probe dot blot were similar to that of cell culture and higher than that of DIA .

  25. 方法对28例SLE患者采用间接免疫荧光法检测CSF及血清中ANA滴度,同时进行CSF的常规、生化检测。

    Methods ANA was examined with indirect immunofluorescence in the sera and CSF of 28 patients with SLE .

  26. 间接免疫荧光法检测糖基化终产物受体(RAGE)。

    The localization of the receptor for AGEs ( RAGE ) in HK-2 ceils was detected by immunofluorescent staining .

  27. 间接免疫荧光法和原位杂交法检测急性白血病中Bcl-2和Bcl-2/Bax表达的对比研究

    Comparision between indirect immunofluorescence assay and in situ hybridization assay in detecting the expression of Bcl-2 and Bcl-2 / Bax in acute leukemia

  28. 免疫荧光法与Giemsa氏法检测沙眼衣原体的比较

    Studies of Giemsa and immunofluorescence staining for detection of Chlamydia trachomatis

  29. 方法免疫荧光法,糖原试验,胶体金标记技术免疫检测,聚合酶链反应(PCR)分别对100例妇产科就诊患者宫颈分泌物标本进行平行检测。

    Methods Immunofluorescence , glycogen test , gold labeling immunoassay and polymerase chain reaction ( PCR ) were used to detect specimen of cervical secretion from 100 women .

  30. 方法:用流式细胞仪根据直接免疫荧光法分别测定病例组与对照组T细胞表面标志CD3,CD4,CD8的表达情况。

    Methods : CD 3 , CD 4 and CD 8 were labeled by immunofluorescence , determined by flow cytometry .