重组子

对PCR扩增的目的片段用1%琼脂糖凝胶电泳检测后，进行回收、连续转化，通过蓝白筛选挑取白色菌落提取质粒DNA进行酶切鉴定，确定重组子后采用双脱氧终止法测定DNA全序列。

By blue white screening , the plasmid DNA extracted from white colony was identified using restriction enzyme analysis and the DNA sequence was determined following recon determination .

细胞融合重组子酵母的研究

Research on Recon Yeast by Cell Fusion

对重组子测序结果表明，实现了DNA小片段和酶切位点的定向引入。

The results showed that the sites and low molecular weight DNA were successfully introduced .

毕赤酵母重组子PCR模板制备方法的比较

Preparation methods ' comparison of recombinant Pichia pastoris Genomic DNA for PCR

PCR技术在鉴定阳性重组子中的应用

Application of PCR technique in the screening of positive recombinants

经PCR鉴定得到重组子。

Identification of transformants obtained by recombinant PCR .

人类白细胞介素2基因重组子cDNA功能片段的碱基序列分析

Nucleotide Sequence Determination of cDNA Fragment of Human Interleukin 2 Gene

构建了目标染色体的DNA文库。文库中大约含有5×10~5个重组子。

The DNA library of the target chromosome were constructed and it contained approximately 5 × 10 ~ 5 clones ;

ColiJM107，获得800多个白色重组子克隆。

Coli strain JM 107 , and more than 800 white clones were obtained .

淋球菌外膜蛋白PI基因重组子的构建和表达

Construction and Expression of the Major Outer Membrane Protein PI of Neisseria Gonorrhoeae in Escherichia Coli

结论：oipADNA重组子能有效诱导抗Hp保护性免疫反应。

Conclusion : oipA DNA could induce effective immune response in protection against Hp infection .

将该重组子转化E.colirosetta。

The recombinant plasmid was transformed into E.coli Rosetta .

AAL层包括会聚子层（CS）和分割和重组子层（SAR）。

The AAL layer comprises the Convergence Sublayer ( CS ) and the Segmentation and Reassembly sublayer ( SAR ) .

数据包捕获与重组子系统用来捕获局域网中流经网卡的web数据包，并对捕获的数据包重组，将其还原成完整的web页面。

Packet capture and reorganization subsystem is used to capture web data packets through the network card in the LAN , and the reorganization of the captured packets , and will restore it into a complete web page .

用人胎肝组织粗提物多克隆抗体对构建的人胎肝cDNA文库进行免疫学筛选，筛选约106重组子后，得到9株阳性克隆。

Recombinant phage from the human fetal liver cDNA expression library were screened by human fetal liver tissues polyclonal antibodies , and nine positive clones were obtained .

将第二轮PCR产物构建质粒文库，得到30000个重组子。

The second-round PCR products were cloned into plasmid vectors to generate a chromosome-specific library consisting of approximately 30000 recombinant clones , from which 84 clones were randomly selected for size evaluation .

重组子经EcoRⅠ酶切验证显示：重组质粒均含有外源DNA插入片段。

The results after digested by restriction enzyme EcoR ⅰ revealed that the recombinants which had been picked out contained foreign DNA fragments .

结果构建成含2.39×106pfu/ml重组子的人大肠癌cDNA噬菌体表达文库，重组率为97.5%。

Results The human colorectal carcinoma cDNA phage library consisting of 2.39 × 10 6 pfu / ml bacteriophages was constructed , and the recombinant percentage was 97.5 % .

结果文库容量为1.9×106个重组子，文库中cDNA插入片段大小介于0.4×103～6.5×103bp。

Results There are 1.9 × 10 6 recombinants in this library . The cDNA fragments ranged between 0.4 × 10 3 bp and 6.5 × 10 3 bp .

coliTAP（106）后，获得pYX（40）对重组子的酶切图谱、原位杂交及DNA序列的分析表明，插入基因的方向、读框正确。

Coli TAP 106 . Analysis of restriction enzyme mapping , hybridizing and DNA sequencing shows that the gene direction and reading frame are correct .

对第2轮PCR产物进行克隆，构建单条染色体DNA文库，经分析，该微克隆文库包含约3×105个重组子。

The second round PCR products from the single chromosome 1 were cloned into T-easy vectors to generate a DNA library of the chromosome 1 . Approximately 3 × 10 ~ 5 recombinant clones were obtained .

重组子成功转染NS-1细胞并表达HBc-Mep融合蛋白。

The cells transfected with the recombinant expressed the combined protein successfully .

将PCR产物连接pMD18-T载体，转化大肠杆菌DH5α，得到了含有目的片段的重组子。

The PCR product was cloned into pMD 18-T Vector , and transformed into Escherichia coli DH5 α cells . The recombinant plasmid was obtained and sequenced .

RTPCR和WESTERNBLOTTING证明，EFE7基因在这些重组子中获得了表达，表达产物相对分子质量高于天然产物。

RT-PCR and Western Blotting showed the gene of component 7 ~ # was expressed in Pichia pastoris , The molecular weight of products expressed were higher than that of the native one .

实验结果表明，诱导表达48h后，Mut+和MutS重组子表达的产物在SDS-PAGE胶上都出现了清晰的目的带。

SDS-PAGE results indicated that there was a clear target band in Muts and Mut + recombinant Culture supernatant after 48 hours culture respectively .

结论：建立了一个编码血红素氧化酶-1基因cDNA重组子（762bp）的真核表达载体。

Conclusion : Expressing vector of the cDNA sequence encoding heme oxygenase 1 gene ( 762bp ) has been determined .

按表达手册进行初步表达实验，经SDS-PAGE检测表明，酵母重组子分泌出了目的蛋白。

The expression experiment is carried out with the methods introduced by expression guideline . According to the result of SDS-PAGE , the target protein is expressed successfully in the Pichia . Pastoris .

采用不同浓度的抗生素G418筛选多重组子，发现在4mg/mL的G418中筛选多重组子效果较好。

With different concentrations of antibiotic G418 selection multiple recombinants , the optimum multiple recombinants were screened from antibiotic G418 ( 4mg / mL ) .

转化子经过表型鉴定，PCR分析和G418浓度梯度筛选获得了高拷贝的重组子（pas-01）。

A high-copy recombinant ( pas-01 ) was screened out from 30 positive transformants by phenotypic identification , PCR analysis and G418 concentration gradient selection .

结果成功构建了TRKC-cDNA质粒，DNA测序证明所获得的目的基因与公布序列的一致性为99%，所获得的重组子符合研究要求。

Results The TRKC-cDNA expressed plasmid was successfully constructed , and DNA sequencing analysis proved that the target gene has good consistency ( 99 % ) with the reported TRKC sequence .