体外重组

DNA浓度对线粒体DNA体外重组的影响

The Effect of DNA Concentration on the Recombination in vitro of Mitochondrial DNA

实验中运用DNA体外重组技术构建了高表达钙调素的真核载体，并将其转染到大鼠正常肾细胞（NRK）中得到钙调素高表达的稳定细胞株。

An eukaryotic vector , which highly expresses calmodulin , was constructed by DNA recombination in vitro and transfected into NRK cells .

纤维蛋白原Bβ链变异基因的体外重组

Extrinsic Recombination of Mutagenic Gene in ? Chain of Fibrinogen B

碱性磷酸单酯酶在DNA体外重组试验中的应用

Application of bacterial alkaline phosphatase ( bap ) to in vitro DNA recombination

应用体外重组PCR技术构建鸡单链抗体基因的研究

Construction of chicken ScFv by recombinant PCR in vitro

真鲷肿瘤坏死因子（TNFα）基因的体外重组与纯化研究

Expression and Purification of Red Seabream Recombinant Tumor Necrosis Factor α in E.coli

pBR（325）质粒DNA与克隆HBVdna体外重组及环丝氨酸富集重组子的研究

In vitro recombination of _pbr_ ( 325 ) plasmid DNA and cloned HBV DNA and the enrichment effects of transformants by cycloserine

另一方面，天然III型干扰素在体外重组基因工程菌种中较难表达和纯化也是制约它们快速取代IFN-α的一个重要因素。

On the other hand , natural type III interferon is more difficult to express and purify in vitro using gene engineering techniques .

体外重组的Rubisco具有8%的恢复活性。

The restored activity of reassembled Rubisco was approximately 8 % of natural enzyme .

目的体外重组人血管内皮生长因子-C（VEGF-C）基因，检验其在细胞中的表达。

Objective To clone human vascular endothelial growth factor-C ( VEGF-C ) gene and detect its expression .

方法依次使用差速离心、热失活、离子交换层析的方法纯化体外重组的人tau蛋白；

Methods Human recombinant tau protein in vitro was purified by using differential centrifugation , heat inactivation and ion exchange chromatography .

为了发展我国的抗衰老和抗艾滋病药物生物技术，研究了大蒜SOD的高效诱导与协同调控以及野生稻TI基因的体外重组与异源表达。

For developing national pharmaceutical biotechnology of anti-ageing and anti-AIDS disease , induced overproduction and cooperative regulation of garlic SOD as well as in vitro recombination and heterologous expression of wild rice TI gene were investigated .

淋球菌mtrR调控区突变片段的体外重组和鉴定

In vitro recombination and identification of mutated fragment corresponding to regulation region of mtrR gene of Neisseria gonorrhoeae

利用纯化的细胞骨架组成蛋白进行体外重组试验，经十二烷基硫酸钠（SDS）电泳结果表明，F-肌动蛋白能侧向与血小板细胞膜结合，但不能与完整血小板结合。

The cytoskeletal component proteins were used in reassembly tests in vitro . Results of SDS electrophoresis showed that ( 1 ) F-actin can reattach to the human blood platelet membrane , but not to the intact platelet .

质粒pBR32与pCRI体外重组的研究

A Study of Recombination of Plasmid pBR322 and Plasmid pCRI in vitro

同时，（His）6-tag的引入不影响apo-protein与heme的体外重组。

At the same time , the ( His ) 6-tag has not affected the in vitro reconstitution of the apo-protein with heme .

突变体基因克隆至表达载体pET30a（+）后，利用体外重组实验对表达蛋白的酶活性进行了研究。

Gene of the deletion mutants was then cloned into expression vector pET 30a ( + ) .

方法采用体外重组基底膜侵袭实验方法，加入不同趋化剂：成纤维细胞条件培养液、纤维黏连蛋白（FN）、条件培养液与FN联合；

Method Reconstituted basement membrane invasion experiment was employed , and conditioned medium , fibronectin ( FN ), and a combination of conditioned medium and FN were respectively used as chemoattractants was placed in the lower compartment of the chamber .

近年来人们发现，油体的三个组成成分TAGs，PLs和OLEOSIN-s，可在体外重组形成稳定的人工油体，人工油体在结构和稳定性上与植物来源的油体无显著差别。

In recent years , reconstitution of a stable artificial oil bodies in vitro , which have no significant difference with native oil bodies in structure and stability , using of the three components of oil bodies-TAGs , PLs and OLEOSINs , has been found .

人血管内皮生长因子-C基因的体外重组和表达

Recombinant Human Vascular Endothelial Growth Factor-C and Its Expression in vitro

人血管生长因子基因片段的体外重组

The Recombination of Human Angiogenin Gene Fragment in Vitro

分离的大鼠睾丸曲细精管体外重组的观察

Observations on the reorganization of the seminiferous tubules of the rat testis in vitro

结论S基因已成功地实现体外重组与表达。

Conclusions The recombination and expression of S gene is achieved successfully in vitro .

体外重组的人促黄体素对小鼠早期胚胎发育的影响

Influence of recombinant human luteinizing hormone on the development of early embryos in mice

本论文还进行了体外重组构建发酵木糖产酒精的酵母工程的初探工作。

The construction of xylose - fermenting yeasts by gene recombinant was studied briefly also .

从体外重组蛋白到潜在的基因兴奋剂的出现，给兴奋剂检测工作带来了巨大的挑战。

From recombinant protein to gene doping , there is a great challenge to their detection .

体外重组基底膜侵袭实验条件及计数方法的探讨

An inquiry to experimental conditions and cell-counting assays in reconstituted basement membrane invasion experiment in vitro

蛋白质定向进化通常分三步进行，即随机诱变、体外重组和筛选。

It is usually based on the combination of random mutagenesis , in vitro recombination and screening .

通过体外重组，将绿色荧光蛋白基因编码序列克隆到鲤鱼肌动蛋白基因启动子下游，构建成能在鱼体内表达绿色荧光蛋白的重组分子。

The coding sequence of green fluorescent protein gene was cloned under the control of carp β - actin gene promoter . A fish expression of green fluorescent protein gene recombinant was constructed .

从不同生物体中切分dna分子重新组合以在体外准备重组dna的技术。

The technology of preparing recombinant DNA in vitro by cutting up DNA molecules and splicing together fragments from more than one organism .