酪氨酸羟化酶

酪氨酸羟化酶在C型尼曼-匹克病小鼠小脑中的异常表达

Abnormal expression of tyrosine hydroxylase in the cerebellum of Niemann-Pick type c mice

铝对大鼠黑质酪氨酸羟化酶mRNA表达的影响

Effect of Aluminum on Expression of Tyrosine Hydroxylase mRNA in the Rat Nigra

采用RT-PCR法检测黑质酪氨酸羟化酶mRNA的表达。

RT-PCR was used to detect expression of TH mRNA .

人酪氨酸羟化酶RNA探针制备的研究

Preparation of a RNA probe for human tyrosine hydroxylase

酪氨酸羟化酶cDNA移植治疗帕金森病的实验研究

Treatment of Parkinson 's Disease by Transplantation of Tyrosine Hydroxylase cDNA : An Experimental Research

酪氨酸羟化酶（TH）是多巴胺合成的限速酶，作为多巴胺的标记。

Tyrosine hydroxylase ( TH ) is the rate-limiting enzyme in the synthesis of dopamine , as a marker of dopamine .

酪氨酸羟化酶（TH）阳性细胞率亦明显增加（P<0.01）。

The ratio of tyrosine hydroxylase ( TH ) - positive neural cells was significantly increased ( P < 0.01 ) .

本文报道用酪氨酸羟化酶（TH）免疫细胞化学方法研究猫生后发育期多巴胺（DA）能神经元的形态和发育。

Morphology and development of dopaminergic neurons has been studied in the kitten retina , using tyrosine hydroxylase ( TH ) immunocytochemistry .

应用免疫组化观察黑质细胞αsynuclein和酪氨酸羟化酶（TH）的表达；

Expressions of α synuclein and tyrosine hydroxylase in nigral cells were investigated by immunohistochemistry .

方法分别将酪氨酸羟化酶（TH）基因的重组表达质粒和重组逆转录病毒导入原代培养的大鼠成纤维细胞和脑胶质细胞，用免疫组织化学检测TH的表达效率；

Methods The recombinant plasmid and retrovirus containing tyrosine hydroxylase ( TH ) gene were transferred into primary rat fibroblasts and glial cells respectively .

db-cAMP对胚胎干细胞来源的神经细胞酪氨酸羟化酶mRNA水平的影响

Effect of db-cAMP on Tyrosine Hydroxylase mRNA level of embryonic stem cell-derived neurons

精神分裂症IL-1β、TNF-α和酪氨酸羟化酶的基因表达水平

The gene expression levels of IL-1 β, TNF - α and tyrosine hydroxylase in peripheral blood mononuclear cell of schizophrenic

16周后用胆碱乙酰转移酶（ChAT）及酪氨酸羟化酶（TH）免疫细胞化学法观察移植物的存活、生长及与宿主海马的关系；

After 16 weeks , ChAT and TH immunocytochemistry were used to observe the survival , growth and connection with host hippocampus of the grafts .

培养7d后，应用酪氨酸羟化酶免疫组化法检测并比较各组多巴胺（DA）能神经元的生长状态。

After 7 day culture , dopaminergic neuronal growth status was examined by tyroxine hydroxylase ( TH ) staining .

目的通过exvivo途径，利用基因工程化细胞对酪氨酸羟化酶（TH）基因在治疗帕金森病中的作用进行探讨。

Objective To explore the role of tyrosine hydroxylase ( TH ) gene in the treatment of Parkinson 's disease ( PD ) by using ex vivo gene transfer .

方法：本实验采用纹状体组织块与胚胎干细胞联合培养的方法，通过形态学观察和酪氨酸羟化酶（TH）免疫细胞化学染色对分化细胞进行鉴定。

Methods : Embryonic stem cell s were co-cultured with striatum explants from newborn mouse brain . The differentiated cells were evaluated by morphological observation and TH immunocytochemical staining method .

免疫细胞化学染色检测神经微管相关蛋白（MAP2）、乙胆碱酯酶（AchE）和酪氨酸羟化酶（TH）的表达，并计算阳性染色细胞的百分率。

MAP_2 , AchE and TH protein were detected using immunocytochemistry respectively and the percentage of positively stained cells was calculated .

NADH能刺激酪氨酸羟化酶和内源性多巴胺的合成，改善帕金森综合症状、对治疗阿尔海姆茨综合症和抑郁症等有效。

NADH stimulates tyrosine hydroxylase and dopamine biosynthesis , and is effective in the Treatment of Parkinson 's Disease .

本实验应用ABC免疫过氧化物酶法，以酪氨酸羟化酶（TH）作为标记物，观察了10只Wistar大鼠脑实质内血管的中枢去甲肾上腺素能神经分布。

The distribution of central noradrenergic nerve in the intracerebral blood vessels of ten Wistar rats was studied with ABC immunoperoxidase technique , employing Tyrosine-hydroxylase ( TH ) as label .

RTPCR分析进一步证明这些细胞能表达γ氨基丁酸（GABA）γ亚单位和酪氨酸羟化酶以及脑因子1神经相关基因。

RT-PCR assay further identified that the induced cells expressed transcripts for neural associated genes including GABA - γ subunit receptor , brain factor-1 and tyrosine hydroxylase ( TH ) .

结果经MPTP腹腔注射后褐鼠模型出现典型PD症状，黑质酪氨酸羟化酶表达水平明显降低，黑质纤连蛋白基因表达明显上调。

Results The PD models were verified successfully by typical symptoms and obvious reduction of tyrosine hydroxylase expression in substantia nigra after peritoneal administration of MPTP .

抗酪氨酸羟化酶（tyrosinehydroxylase，TH）抗体免疫组织化学染色显示黑质多巴胺（DA）能神经元，光镜下观察并进行细胞计数，统计学分析处理数据。

The antibody to tyrosine hydroxylase ( TH ) was used to label dopamine ( DA ) neurons . The morphological study and cell count were performed under microscope .

Lactacystin对酪氨酸羟化酶基因和蛋白表达的影响

Effects of Lactacystin on the Expressions of Gene and Protein of Tyrosine Hydroxylase

方法：采用免疫组化技术和高效液相-电化学法（HPLC-EC）分别对酪氨酸羟化酶（TH）、DA进行了含量测定。

Methods : Immunohistochemistry technology and high-pressure liquid chromatography with electrochemical detector ( HPLC-EC ) were used to evaluated tyrosine hydroxylase ( TH ) and DA .

于移植后3d、1月、3月将动物处死，采用酪氨酸羟化酶（TH）免疫组化法观察3组动物移植部位DA能神经元的存活和生长状况。

Three days , one month , three months after grafting , the rats were sacrificed and used for tyrosine hydroxylase ( TH ) immunohistochemical staining . The fetal kidney transplantation increased the survival of dopaminergic neurons .

在外周压力感受器去神经支配的大鼠上，用Fos蛋白和酪氨酸羟化酶（TH）的双重免疫组化方法，研究辣椒素的效应是否通过激活脑干核团内儿茶酚胺能神经元而诱发。

The present study was undertaken to define whether the effects of capsaicin were induced by activating the catecholaminergic neurons by double immunohistochemical method for Fos and tyrosine hydroxylase ( TH ) .

目的：探讨酪氨酸羟化酶（TH）蛋白在肾上腺髓质增生（AMH）大鼠肾上腺组织中的表达与肾上腺髓质增生的关系。

Objective : To study the relationship between the expression of tyrosine hydroxylase ( TH ) in adrenals and adrenal medulla hyperplasia ( AMH ) in rats .

结论进一步证明了黑质细胞凋亡是早期帕金森病模型大鼠黑质细胞死亡的主要方式之一，是纹状体区酪氨酸羟化酶（TH）减少的主要原因。

Conclusions Apoptosis is the main way of nigral dopaminergic neurons ' death in acute course of PD models , and is the main cause leading to the decrease of tyroxine hydroxylase ( TH ) in striatum .

将人酪氨酸羟化酶cDNA表达盒克隆于质粒型腺病毒载体p△Elsp1A，得到重组质粒pAd－TH。

The human tyrosine hydroxylase ( TH ) cDNA containing expression cassette was inserted into adenovirus plasmid-type vector of p △ Elsp1A , to generate a recombinant plasmid pAd-TH as transfer vector .

用酪氨酸羟化酶免疫荧光评价MPTP对黑质多巴胺能神经元的毒性。

Tyrosine hydroxylase ( TH ) immunofluorescence was used to evaluate the toxicity of MPTP on the neural cells in the substantia nigra .